Labshake search
Citations for Beckman :
901 - 950 of 1934 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries corresponding to samples to be directly compared to each other (e.g. DMSO vs UNC7040) were pooled together and purified using 1 volume of Agencourt AMPure XP (Beckman Coulter). The pooled libraries were eluted with 23 µL of elution buffer (NEXTflex ChIP-Seq kit ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... the sample was incubated at 25°C for 1 hour and then cleaned up by performing two rounds of 1.2x SPRI Select (Beckman Coulter). The sample was then subject to bisulfite conversion following the recommended protocol of Zymo EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was purified using RNA XP beads purification with 1:1.8x ratio of sample to beads (Beckman Coulter, A63987) and eluted in 24.5 µl water ...
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bioRxiv - Neuroscience 2021Quote: ... and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter). Supernatant were collected by pipetting ...
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bioRxiv - Genomics 2020Quote: ... This sequencing-ready NGS library was purified using 1 round of a 1X proportion of AMPure XP beads (Beckman Coulter). The final elution volume was 17 µl in Elution Buffer.
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bioRxiv - Microbiology 2021Quote: ... followed by purification with a 2.2:1 volumetric ratio of RNAClean XP solid phase reverse immobilization (SPRI) beads (A63987, Beckman Coulter). Copy numbers of resulting cDNAs were determined by limiting-dilution PCR using fluorescence-assisted clonal amplification (FCA ...
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Cell Biology 2023Quote: A sucrose gradient was prepared by mixing 3 ml of liver homogenate with 1 ml of HLM containing 60% sucrose in a 13.2 ml ultracentrifuge tube (Beckman Coulter), followed by 5 ml and then 4 ml of HLM buffer containing 5% and 0% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were purified using a ratio of 1:0.85 sample to bead ratio using Agencourt AMPure XP SPRI beads (Beckman Coulter). The final products were quantified on a D5000 Tapestation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified according to the previously mentioned bead clean-up procedure (using 0.6 to 1-fold AMPure XP bead suspension, A63880, Beckman Coulter), except that after removal of ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transposition was blocked by the addition of 1% SDS and DNA purified with Agencourt AMPure XP beads (Beckman Coulter, A63881) according to the manufacturer’s instructions using a 2:1 ratio of beads to sample ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Microbiology 2023Quote: ... PBS-washed cells were PFA-fixed for 90 minutes and immunostained with the following antibodies diluted in 0.1% Triton X-100 in PBS: anti-HIV-1-core-antigen-FITC or -PE (#6604665 or #6604667; Beckman Coulter), rabbit-anti-human-MX1/2 (#sc-166412 ...
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bioRxiv - Microbiology 2024Quote: ... Additional aliquots of seawater taken from the same CTD casts used for cell-specific rate incubations were 1) preserved in paraformaldehyde (0.5% final concentration) for later shore-based analyses on a CytoFlex S flow cytometer (Beckman Coulter) after staining with Hoechst 34580 (Selph 2021) ...
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bioRxiv - Cell Biology 2023Quote: ... tared and ultracentrifuged for 1 h at 110,000x g using a Beckman L7-65 ultracentrifuge with a SW41Ti rotor (#331362, Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...
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bioRxiv - Cell Biology 2022Quote: ... lipoprotein particles (LPPs) were purified from the non-sedimentable fraction after 1 h of ultracentrifugation in a Ti-45 rotor (Beckman) at 44,000 rpm at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... followed by 72°C for 1 minute followed by purification with 1X Agencourt AMPure XP beads as per the manufacturer’s instructions (Beckman Coulter).
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bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 15 000 RPM for 1 minute using an AllegraTM X-22R Centriuge (Beckman Coulter™, California, USA) to ensure all reagents were concentrated at the bottom of the wells ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Synthetic Biology 2024Quote: ... then approximately 1/10th of the volume of each pool was purified with a 0.9:1 ratio of AMPure XP beads:PCR mix (Beckman Coulter, A63881). The complete volume of the AMPure-cleaned 1st round PCR products for each library was used as the templates for a “2nd round PCR,” which added the remainder of the TruSeq HT i5 and i7 Illumina adaptor sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The discontinuous gradient was spun for 18 hours at 100,000xg and collected into twelve 1 mL fractions (SW40 Ti rotor, Beckman Coulter). SEVs are typically located in fractions 6 and 7 ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution).
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... 1 million peripheral immune cells were pipetted into the DuraClone immunophenotyping tube (DuraClone IP Phenotyping kit from Beckman Coulter, USA). Each tube contained anti-CD45-KO ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular Gag staining was performed in PBS+1%BSA+0.05%Saponine using the KC-57 anti-Gag antibody (Beckman-Coulter) for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed two times with PBS supplemented with BSA (1%) and data recorded in a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Microbiology 2024Quote: ... cells were washed in PBS containing 1% BSA and resuspended in PBS containing 0.5% BSA before fluorescence quantification by CytoFlex S Benchtop Flow Cytometer (Beckman Coulter).
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bioRxiv - Genetics 2024Quote: ... An aliquot of each cell suspension was diluted 1:1,000 with seawater and cell count was performed using flow cytometry (CytoFLEX S, Beckman Coulter) set at medium flow (30 µl/min) ...
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bioRxiv - Biophysics 2024Quote: ... 100 μL of Origami-R1-R2 was placed on top of a quasi-linear glycerol gradient (15–45%, in 1× TE-Mg2+ buffer) in a 0.8 mL Open-Top Thinwall Ultra-Clear tube (Beckman Coulter) and spun in an SW 55 Ti rotor (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Immunology 2022Quote: ... All experiments in this study were performed using a 3-laser CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA, USA) and operated using the CytExpert Software v2.4.0.28 (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... The harvested sEVs were resuspended with 3 mL double filtered PBS and centrifuged with MLA-55 rotor (Beckman Coulter) under 100,000 × g at 4 °C for 70 mins to remove non-EV contaminants ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...