Labshake search
Citations for Beckman :
1051 - 1100 of 1302 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Genomics 2022Quote: ... The eluted chromatin was reverse-crosslinked overnight at 65°C and the DNA was purified with AMPure XP beads (Beckman Coulter).
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bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
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bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
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bioRxiv - Cancer Biology 2024Quote: ... using a combined Annealing/Extension step at 63°C for 10 seconds and 15 cycles followed by a 1.1X post-amplification AMPure XP (Beckman Coulter, A63880) bead cleanup ...
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bioRxiv - Molecular Biology 2024Quote: ... the fixed samples were subjected to sucrose cushion centrifugation (7,000x g for 20 min at 16 °C) using a swinging bucket rotor (Beckman, JS-7.5). Coverslips were recovered ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
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bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
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bioRxiv - Molecular Biology 2024Quote: ... and 1U of FastAP (TFS, EF0654) for 15 min at 37 °C and purified using 1.2× SPRIselect beads (Beckman Coulter, B23318). The inDrops-v1.1 libraries were digested with 40U of ExoI and 1U of FastAP for 15 min at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged at ∼14.000 x g max (∼10900 x g average) for 22 min at 4 °C (Beckman Coulter, MAX-XP). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Microbiology 2024Quote: ... incubated at 25°C and 5% CO2 (PF) or 37°C and 5% CO2 (BF) for 60 min and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). Events (10,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Microbiology 2023Quote: ... The isopycnic centrifugation of DNA was performed with an initial CsCl buoyant density of 1.725 g mL-1 subjected to centrifugation at 177,000 × g for 36-40 h at 20 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter, USA). At the end of the centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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bioRxiv - Biophysics 2023Quote: ... and concentrated by several washing steps in FBS-free Opti-MEM® and virus particles were concentrated by ultracentrifugation on a 60% (w/V) iodixanol cushion at 21°C (131,500 x g, 90 min, SW28 rotor, Beckman Coulter, Ireland) to a final volume of 1 mL ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Plant Biology 2023Quote: ... for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter); 1D2 Adapters (ONT ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...