Labshake search
Citations for Beckman :
1001 - 1050 of 1670 citations for 7 Hydroxy 4 methoxy 1 indanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were ultracentrifuged at 120,000 to 140,000 x g (SW-28 rotor) for 2 hours at 4°C to pellet EVs on an OptimaTM L-100 XP ultracentrifuge (Beckman Coulter). The supernatant was removed ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in PBS and stored in the dark at 4°C until analyzing them with a Navios flow cytometer (Beckman Coulter). At least 10’000 living cells were measured for each sample ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was then ultracentrifuged at 50,000 RPM and 4 °C for 90 minutes using a 70.1 Ti rotor (Beckman Coulter, Brea, CA, USA) with 1 mL 15% sucrose cushion per tube ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 60,000 rpm at 4°C for 2hr in an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The translucent polysome pellets were washed by gently dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... and 1.5 ml of this was added to the top of the sucrose gradient and was centrifuged at 69,150 x g for 70 min at 4°C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. # 331362). 1–1.5 ml of the synaptosome layer was collected from the interface of the two sucrose layers ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...
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bioRxiv - Immunology 2022Quote: ... Ultracentrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2 hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, CA, USA) was performed to pellet the viruses through the sucrose cushion while soluble egg proteins were removed ...
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bioRxiv - Microbiology 2020Quote: ... the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter, Indianapolis, IN) to remove unbound stain ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Microbiology 2020Quote: ... were further purified by centrifugation through a 1.2 g/cm3 to 1.6 g/cm3 cesium chloride density gradient at 50,000 RPM for 4 hours at 11 °C in a Beckman SW 65 Ti rotor (Beckman Coulter, Brea, California). Visible “virus bands” in the gradients were collected using a Hamilton syringe and diluted 1:10 in 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... virions were purified from the infected S2 cells by centrifugation at 8,000 RPM for 10 minutes at 4 °C using a Beckman GSA rotor (Beckman Coulter, Brea, California). The supernatant was then collected and centrifuged at 8,000 RPM for 30 minutes at 4 °C using a Beckman GSA rotor ...
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bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
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bioRxiv - Immunology 2021Quote: ... Cell debris was removed by centrifugation (45 min at 18,000 rpm, at 4°C; Avanti® J-26xp centrifuge (BECKMAN COULTER®)) ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 x g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter, Brea, CA). The cell pellet was resuspended in 5 ml ice-cold PBS and stored at −80 °C for further use ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45.000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter, Brea, CA). The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 100 000 × g for 60 min at 4°C using rotor Ti-70 in an Optima LE-80K Ultra Centrifuge (Beckman Coulter, Australia). The harvested EV pellet was resuspended in 1 mL RNase-free Phosphate buffered saline (RNase-free PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviruses were purified by overlaying the virus containing media over a 20% sucrose solution before ultracentrifugation at 26,000 rpm for 90 minutes at 4 °C in a SW28Ti swinging-bucket ultracentrifuge rotor assembly in an Optima XPN-80 Ultracentrifuge (Beckman Optima Ultracentrifuge) and the viral pellet was resuspended in AimV™ media.
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 10,000 x g for 30 min at 4°C and the supernatants were transferred to ultracentrifuge tubes (Beckman Coulter, Brea, CA) and diluted with phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... All bacteria were harvested by centrifugation at 4000 rpm and 4°C in a JS 4.0 swinging bucket rotor (Beckman Coulter, Brea California) and immediately processed as described below.
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bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting supernatant was centrifuged at 10,000 x g for 30 minutes to remove any remaining debris and then ultra-centrifuged at 118,000 x g for 70 minutes at 4°C with a fixed-angle rotor (Ti-70, Beckman Coulter, Brea, CA). The pellet was washed with sterile 1X PBS and subjected to another cycle of ultracentrifugation at 118,000 x g for 70 minutes at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... re-suspend in the homogenization buffer and centrifuged at 100,000 x g for 40 min at 4°C (Beckman SWTi41 swing rotor). The pellet containing the growth cone were re-suspended in 50 μl of the homogenization buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was again centrifuged at 100000 g for 45 min at 4°C (Beckman Optima™ MAX-XP Rotor MLS-50). After filtering the supernatant through a 0.22 μm syringe filter ...