Labshake search
Citations for Beckman :
851 - 900 of 1670 citations for 7 Hydroxy 4 methoxy 1 indanone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... The lysate was clarified by centrifugation at 40,000 rpm for 35 min at 4°C (Type 45 Ti Rotor, Beckman Coulter). The resulting supernatant was mixed with 1ml pre-washed IgG Sepharose 6 FastFlow beads (Cytiva ...
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bioRxiv - Cell Biology 2023Quote: ... and pelleted at 100,000xg for 20 min at 4 °C using fixed angle rotor (TLA-100 rotor, Beckman Coulter, 349481) in a benchtop ultracentrifuge (OptimaTL ...
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bioRxiv - Neuroscience 2023Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Immunology 2023Quote: ... virus solutions were clarified of debris by centrifugation at 10,000 rpm at 4°C for 30 minutes in a chilled SW32Ti rotor (Beckman Coulter), followed by pelleting through a 30% cold sucrose solution at 25,000 rpm for 90 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Medium was cleared from EVs by ultracentrifugation at 100,000 ×g for 18 h at 4°C in a LE-80 ultracentrifuge (Beckman Coulter) with a 60Ti rotor [13] ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with an 18G needle ...
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bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
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bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
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bioRxiv - Biochemistry 2023Quote: ... The diluted extract was then centrifuged at 31,200 rpm for 30 minutes at 4°C in a Type 70 Ti rotor (Beckman Coulter). The supernatant was filtered through a 0.45 µm syringe filter (Millex-GP PES membrane (Millipore SLHP033RS ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation at 4℃ at 184,000 x (g) in a 70Ti rotor for 50 minutes (Beckman Coulter). After affinity chromatography with StrepTactin SuperFlow resin (IBA) ...
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bioRxiv - Molecular Biology 2024Quote: ... and EVs were pelleted by ultracentrifugation for 60 minutes at 100,000 x g at 4 ° C in an SW40.Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... A crude ribosome pellet was obtained by centrifugation for 2h at 200,000 × g at 4 °C (70Ti rotor, Beckman Coulter) and solubilized in HMA6/30 for 16h at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and pelleted each time by ultracentrifugation at 100,000 x g for 70 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged at 10,000 x g for 30 min at 4°C using a TLA100.3 fixed angle rotor and an Optima TLX Ultracentrifuge (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... passed through 0.45 µm filters and concentrated on a 20% sucrose cushion (24,000 rpm at 4 °C for 2 h with a SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were added to the top of a 60%-30%-10% sucrose/PBS gradient and centrifuged at 16,000 x g for 4.5 h at 4°C with an SW32 Ti rotor (Beckman, Brea, CA). Virus bands at the 60%-30% sucrose interface were collected via tube side puncture ...
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bioRxiv - Microbiology 2024Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2024Quote: ... tepidum culture was centrifuged at 10,000 x g for 10 min at 4°C in a JA-14 rotor (Beckman Coulter), and the supernatant was centrifuged again at 12,000 x g to remove cells and large debris ...
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bioRxiv - Microbiology 2024Quote: ... The pellets from different centrifuge tubes were pooled and resuspended in 1 mL of 1X PBS and pelleted again by ultracentrifugation at 165,700 x g for 2 h at 4°C in a TLA-55 rotor (Beckman Coulter). The pellet was resuspended in 500 µL of 1X PBS and stored at -20°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant was ultracentrifuged at 100,000 g for 2 hours at 4°C with a 100 Ti rotor (Beckman Coulter) in a Beckman XL-90 ultracentrifuge ...
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bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation for 35 minutes at 4℃ at 70,000 x (g) in a 45Ti rotor (Beckman Coulter). Constructs containing N-terminal TOG domains (TOG1 ...
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bioRxiv - Microbiology 2024Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge; Beckman Coulter). The supernatant was discarded ...
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bioRxiv - Neuroscience 2024Quote: ... The discontinuous sucrose gradient was centrifuged at 242,000 g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Immunology 2024Quote: ... and centrifuged at 100,000 x g at 4°C for 90 min using the Optima™ XPN-80 Ultracentrifuge from Beckman Coulter ...
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bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were subjected to ultracentrifugation of 260,000 x g for 16 hours at 4°C using the Optima XPN-80 and SW40Ti rotor (Beckman Coulter). Twelve 1-mL fractions were collected from the top and subjected to immunoblotting.
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bioRxiv - Biophysics 2024Quote: ... the sample was separated by sucrose gradient ultracentrifugation with the gradients of 10 to 30 % sucrose and 0 to 0.2 % glutaraldehyde at 97,311 g at 4°C for 18hrs using an Optima™ XL-100K ultracentrifuge (Beckman Coulter). The fractions containing the complex were pooled and the sucrose was removed by using Amicon Ultra Centrifugal Filter ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The gradients were centrifuged at 40,000 rpm for 2 h at 4°C using a SW40 Ti rotor (Beckman Coulter). Eleven fractions of 500 µl each were collected and 200 µl and 50 µl were used for RNA and protein analysis ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant from the 10,000xg spin was layered on top of a 2 mL 60% iodixanol cushion and spun for 4 hours at 100,000xg (SW32 Ti rotor, Beckman Coulter). The bottom 3 mL of the cushion was obtained and layered at the bottom of a discontinuous gradient ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
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bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
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bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
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bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).