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Citations for Beckman :
1001 - 1050 of 1789 citations for 1 Piperidin 4 yl azetidine 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and samples were centrifuged at 24,000 rpm at 4°C for 1h in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). After discarding the supernatant ...
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bioRxiv - Molecular Biology 2024Quote: ... and EVs were pelleted by ultracentrifugation for 60 minutes at 100,000 x g at 4 ° C in an SW40.Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation at 4℃ at 184,000 x (g) in a 70Ti rotor for 50 minutes (Beckman Coulter). After affinity chromatography with StrepTactin SuperFlow resin (IBA) ...
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bioRxiv - Biochemistry 2024Quote: ... A crude ribosome pellet was obtained by centrifugation for 2h at 200,000 × g at 4 °C (70Ti rotor, Beckman Coulter) and solubilized in HMA6/30 for 16h at 4°C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... EVs were purified from concentrated HES by ultracentrifugation at 100,000 x g for 90 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and pelleted each time by ultracentrifugation at 100,000 x g for 70 mins at 4 °C in polyallomer tubes (Beckman Coulter) in a SW40 rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were clarified by ultracentrifugation for 35 minutes at 4℃ at 70,000 x (g) in a 45Ti rotor (Beckman Coulter). Constructs containing N-terminal TOG domains (TOG1 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... containing cell nucleus and other heavy organelles was discarded and supernatant was centrifuged for 30 min at 48,000 × g at 4 °C (Beckman Avanti J-251 Ultra-centrifuge; Beckman Coulter). The supernatant was discarded ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant was ultracentrifuged at 100,000 g for 2 hours at 4°C with a 100 Ti rotor (Beckman Coulter) in a Beckman XL-90 ultracentrifuge ...
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bioRxiv - Immunology 2024Quote: ... and centrifuged at 100,000 x g at 4°C for 90 min using the Optima™ XPN-80 Ultracentrifuge from Beckman Coulter ...
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bioRxiv - Biophysics 2024Quote: ... the sample was separated by sucrose gradient ultracentrifugation with the gradients of 10 to 30 % sucrose and 0 to 0.2 % glutaraldehyde at 97,311 g at 4°C for 18hrs using an Optima™ XL-100K ultracentrifuge (Beckman Coulter). The fractions containing the complex were pooled and the sucrose was removed by using Amicon Ultra Centrifugal Filter ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were subjected to ultracentrifugation of 260,000 x g for 16 hours at 4°C using the Optima XPN-80 and SW40Ti rotor (Beckman Coulter). Twelve 1-mL fractions were collected from the top and subjected to immunoblotting.
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cancer Biology 2024Quote: ... The gradients were centrifuged at 40,000 rpm for 2 h at 4°C using a SW40 Ti rotor (Beckman Coulter). Eleven fractions of 500 µl each were collected and 200 µl and 50 µl were used for RNA and protein analysis ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant from the 10,000xg spin was layered on top of a 2 mL 60% iodixanol cushion and spun for 4 hours at 100,000xg (SW32 Ti rotor, Beckman Coulter). The bottom 3 mL of the cushion was obtained and layered at the bottom of a discontinuous gradient ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... CD14 BV605 (1:100) and CD11b PC5 (1:100, Beckman Coulter). Cells were analyzed on the FACSCelesta (BD).
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bioRxiv - Systems Biology 2022Quote: ... and purification with Ampure XP beads (1:1 ratio, Beckman Coulter) produced a purified cDNA library ...
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bioRxiv - Genomics 2020Quote: ... cleaned up with 1:1 AMPureXP beads (Beckman Coulter, Brea, CA). ...
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bioRxiv - Plant Biology 2020Quote: ... purified with the double AMPure XP cleanup (1:1) (Beckman Coulter), and checked with a High sensitivity DNA chip (Agilent technologies) ...
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bioRxiv - Plant Biology 2021Quote: ... the samples were centrifuged at 35 000 RPM for 18 hours at 4°C in a 70Ti rotor and L8-M ultracentrifuge (Beckman Coulter). The ribosome pellets were then gently washed with RNase-free water and resuspended in 300 μl of resuspension buffer (100 mM Tris·HCl (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Molecular Biology 2021Quote: ... The gradient was ultracentrifuged at 55,000 rpm for 55 min at 4 °C with max acceleration and slow deceleration using an Optima LE-80K Ultracentrifuge and SW55Ti rotor (Beckman Coulter). The sucrose gradient was fractionated according to the number of associated ribosomes as determined by the profile of the absorbance at 254 nm using a Density Gradient Fractionation System (Brandel ...
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bioRxiv - Neuroscience 2021Quote: ... This was incubated for 1 hour at room temperature while rotating before centrifugation at 100,000 x g for 90 minutes at 4°C using the Optima™ MAX-XP Ultracentrifuge (Beckman Coulter). The supernatant was then collected (sarkosyl-soluble fraction) ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were pelleted down for 10 min at 5000 rpm at 4°C (Rotor: F10-6X500, FiberLite Beckman J2-MC). The pellet was washed with buffer 1 (HEPES- 20 mM ...
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bioRxiv - Bioengineering 2022Quote: ... The 60-70 mL were then divided equally into two 50-mL centrifuge tubes and spun down outside the anaerobic chamber at 4 °C and 3500 rpm for 10 min using an Allegra 64R ultracentrifuge (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2022Quote: ... Each 250 mL culture was divided equally into six 50-mL centrifuge tubes and spun down at 4 °C and 3500 rpm for 10 min using an Allegra 64R centrifuge (Beckman Coulter). The supernatant was discarded and 6 mL of ice-cold 10% glycerol was used to wash and combine the six cell pellets into one suspension ...
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bioRxiv - Genomics 2020Quote: ... Lentiviral supernatant was collected at 48 and 72hrs post-transfection and subsequently concentrated by ultracentrifugation (24,000 rpm, 4°C, 2hrs) (Beckman Coulter SW32).
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bioRxiv - Genetics 2020Quote: ... The homogenates were incubated on ice for 15–20 min and then centrifuged at 3000xg for 10 min at 4°C in a GPR Data Settop centrifuge (Beckman, Inc.). The supernatant solutions were centrifuged at 15,800xg for 15 min at 4°C in a microcentrifuge (Model 5042 ...
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bioRxiv - Plant Biology 2020Quote: ... The loaded density gradients were centrifuged at 33,000 g for 14.5 h at 4°C using a six-position SW41 Ti rotor (Beckman Coulter, Kerfeld, Germany). The positions of ribosome complexes varied slightly with each centrifugation run that comprised a set of six samples ...
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bioRxiv - Bioengineering 2020Quote: ... Ultracentrifugation was performed at 100,000 x g at 4 C for 45 min in an Optima MAX-XP ultracentrifuge (Beckman Coulter, Inc.). Supernatant was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
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bioRxiv - Biophysics 2021Quote: Purified 3C DNA from each sample was sonicated to 200-400 bp with a Soniprep 150 probe sonicator at 4°C and purified with a standard Ampure XP Bead protocol (Beckman Coulter) using a 1/1.5 DNA to bead ratio ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was then collected and subjected to ultracentrifugation at 100,000 x g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter). Pellets were washed in PBS 1X and EVs were subjected to an additional centrifugation at 100,000 x g for 30 min at 4°C using an Optima MAX ultracentrifuge ...
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bioRxiv - Neuroscience 2020Quote: ... centrifuged at 100,000 g for 70 min at 4°C using an Optima XPN-80 ultracentrifuge with SW 32 Ti swinging rotor (Beckman Coulter) and then processed for further analyses.
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bioRxiv - Plant Biology 2021Quote: ... Syringes were placed inside 50-mL tubes and centrifuged for 20 min at 700g with slow acceleration (4°C, JA-14 rotor, Avanti J-20 XP Centrifuge; Beckman Coulter). The apoplastic wash fluid was then filtered through a 0.22 μm membrane and centrifuged at 10,000g for 30 minutes to remove any remaining large particles ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% Paraformaldehyde for 30 minutes at room temperature and analyzed for GFP fluorescence on a Gallios flow cytometer (Beckman-Coulter). For SARS-CoV-2 experiments ...
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bioRxiv - Molecular Biology 2021Quote: ... and spung for 20hrs at 151,000 x g (rav) at 4°C in a swinging bucket rotor (Beckman SW-41 Ti). 12 1ml fractions were taken off the gradient and incubated for 10min at RT with 100μl 0.15% deoxycholine ...
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bioRxiv - Microbiology 2021Quote: ... and cytoplasmic membranes were precipitated by ultracentrifugation for 45 min at 55,000 rpm and 4°C (Beckman Coulter rotor TLA 55). Membrane fractions resuspended in resuspension buffer (750 mM aminocaproic acid ...
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bioRxiv - Biophysics 2021Quote: ... The crude lysate was sonicated on ice and clarified by centrifugation for 30 min at 4°C and 35,000 rpm in a Beckman L90K Optima and 50.2 Ti rotor (Beckman, Brea, CA, USA). The clarified lysate was applied to a HisTrapHP column (Cytiva ...
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bioRxiv - Biophysics 2021Quote: ... and the fractions containing the protein of interest were combined and concentrated to 2mL at a final concentration of 3.85 mg/mL by repeated centrifugation in an Amicon Ultra Centrifugal Filter at 4k rpm for 5 min at 4 °C (Beckman SX4250). The concentrated protein was incubated overnight with TEV (1:50 mass ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... vehicle control or compounds from the small molecule library (Supplementary Table 4) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were then incubated in an IncuCyte Zoom (Sartorius ...
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bioRxiv - Microbiology 2022Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated fibers that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous sucrose gradient (40% ...
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bioRxiv - Microbiology 2022Quote: ... through a syringe needle (0.4-by 20 mm needles) and the cell wall was collected by ultracentrifugation at 90,000 × g for 45 min at 4°C (Beckman Coulter, Inc.). To the pellet ...