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Citations for Beckman :
951 - 1000 of 2168 citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged at 100,000 x g either for 4 hr (hollow fiber bioreactor) or overnight (2D flask culture) in a SW32 rotor (Beckman Coulter). For the preparation of the density gradient ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... and ultracentrifuged at 200,000 × g for 4 hours using a SW 55 Ti rotor in a Beckman OPTIMA90X ultracentrifuge (Beckman Coulter, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged at ∼14.000 x g max (∼10900 x g average) for 22 min at 4 °C (Beckman Coulter, MAX-XP). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
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bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Cell Biology 2024Quote: ... The samples were ultracentrifuged at 39,000 rpm at 4°C for 2.5 h in an SW41 rotor (Beckman Coulter, California, USA). Finally ...
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bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
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bioRxiv - Immunology 2023Quote: ... and B cells were determined using Flow-Count fluorospheres (Beckman) and a Cytoflex flow cytometer (Beckman ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... was clarified by centrifugation at 10,000 rpm for 30 min at 4 °C using an SW-28 rotor in an Optima L-90K Ultracentrifuge (Beckman Coulter, USA) and was then ultracentrifuged at 25,000 rpm for 6 h at 4 °C using the same rotor ...
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bioRxiv - Molecular Biology 2019Quote: ... The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Density fractions of 1 ml were collected and fractions 9-10 pooled ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was then ultracentrifuged at 50,000 RPM and 4 °C for 90 minutes using a 70.1 Ti rotor (Beckman Coulter, Brea, CA, USA) with 1 mL 15% sucrose cushion per tube ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 60,000 rpm at 4°C for 2hr in an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The translucent polysome pellets were washed by gently dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2019Quote: ... The filtrate was then re-centrifuged at 125000 × g at 4° C for 3 hours (Optima™ L-XP ultracentrifuge, Beckman, USA). The obtained pellet was suspended in 300 µl sterile phosphate-buffered saline (PBS) ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
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bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
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bioRxiv - Neuroscience 2020Quote: ... and 1.5 ml of this was added to the top of the sucrose gradient and was centrifuged at 69,150 x g for 70 min at 4°C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. # 331362). 1–1.5 ml of the synaptosome layer was collected from the interface of the two sucrose layers ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...