Labshake search
Citations for Beckman :
951 - 1000 of 1337 citations for 7 Nitro 3 4 dihydro 2H benzo b oxepine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sucrose cushion was ultra-centrifuged for 1.5 hours at 80000 rpm and 4°C in TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 100 µL of buffer F (50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The resulting gradient was then subjected to centrifugation for 16 hours at 150,000 x g and 4 °C (SW55 Ti rotor, Beckman-Coulter). Sample banding was visually observed at the interface of the 30% and 20% fractions (Supporting Information ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Cell Biology 2024Quote: ... The step gradients were centrifuged for 5 h at 132.000 x g at 4 °C (SW60 Beckman and Coulter, # 335649). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). Pellets containing fixed chromosomes were resuspended with 10 mL of buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). The chromosomes were collected from the bottom of the centrifuge tube and resuspended with buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The washed insoluble pellets were resuspended in 8 mL of 6 M guanidine HCl (pH 1.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The denatured proteins were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). Protein concentrations in soluble fractions were estimated based on the absorbance at 260 nm ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... aggregated proteins were removed by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was mixed with 62.7 g of solid ammonium sulfate and stirred overnight at 4 ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture was spun at 13,894 rpm (16,000 rcf) at 4 ºC for 10 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The beads that accumulated at the bottom of the tube were resuspended in 200 µL of EM buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... they were collected by centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 20 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... and spun at 32,000 rpm (max 124,436 rcf) and 4 ºC for 13 h using an SW55Ti rotor in an Optima L80 centrifuge (Beckman Coulter). The samples were fractionated from the top of the sucrose gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... Insoluble fractions were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 30 min using an SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The supernatants were collected and layered onto a 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Molecular Biology 2024Quote: ... Insolubilized proteins were removed with centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was loaded into the HisTrap HP column (Cytiva) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... the fifth virus was freeze-thawed three times and ultracentrifuged at 23 k rpm at 4 °C for 1.5 hours in the Super-centrifuge Optima XE-90 Ultracentrifuge (Beckman Coulter) using Ultra-Clear Centrifuge Tubes (Beckman Coulter) ...
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bioRxiv - Bioengineering 2023Quote: ... The final supernatant was centrifuged at 100,000g at 4°C for 70 min using an ultracentrifuge (Optima MAX-XP Tabletop Ultracentrifuge, Beckman Coulter). The pellet was either used immediately or stored dry at −80°C ...
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bioRxiv - Biochemistry 2023Quote: ... and the mixtures were then layered under a 20-40% iodixanol discontinuous gradient before to be centrifuged at 260,000 x g for 16h at 4°C using an SW41 Ti rotor (Beckman Coulter). After spinning ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Biophysics 2023Quote: ... Centrifugation was performed at 154,300 × g for 24 h at 4◦C (SW 41 Ti swinging-bucket rotor, Beckman Coulter). The separated bands were collected dropwise from the bottom of the tube ...
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bioRxiv - Immunology 2022Quote: ... eVLPs were purified by ultracentrifugation at 50,000 rpm (135,000 x g) for 2 hours at 4°C using a TLA100.3 rotor and a OptimaTM TLX ultracentrifuge (Beckman Coulter) on a 20% w/v sucrose cushion ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 25 000 rpm for 45 min at 4°C in a Ti45 rotor (Beckman). Supernatant was incubated with 5 ml of Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenised mixture then underwent ultracentrifugation at 200,000 g for 110 min at 4°C with a SW-41 Ti rotor (Beckman Coulter) and transferred the supernatant into a Slide-A-Lyzer™ G2 Dialysis Cassettes 2K MWCO (Thermo ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Microbiology 2022Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minute 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). For SDS-PAGE analyses ...
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bioRxiv - Cell Biology 2023Quote: ... The iodixanol density gradients were centrifuged at 100,000 x g at 4◦C for 18 hours in SW41Ti rotor (Beckman Coulter). Ten fractions (F1 ...
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bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times though 2mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed by sonication and the lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The lysate was passed three times through 2 mL of pre-equilibrated Ni-NTA Agarose beads (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 45 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 150,000 rcf for 30 min at 4°C using a Type 45 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysate was clarified at 503,000 rcf for 45 min at 4°C using a Type 70 Ti Rotor (Beckman Coulter). The supernatant was incubated with 3 mL IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... then pelleted at 4°C and 4,300 x g for 30 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.