Labshake search
Citations for Beckman :
51 - 100 of 1537 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
-
bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
-
bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
-
bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
-
bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
-
bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
-
bioRxiv - Cell Biology 2021Quote: ... After centrifugation (285, 000 g for 2 h at 4 °C using a Beckman SW40Ti rotor), polysome profiles were recorded using an Äktaprime plus chromatography system (GE Healthcare) ...
-
bioRxiv - Microbiology 2022Quote: ... 4°C for 2 h (SW 32 Ti swinging-bucket rotor, Optima LE-80K Beckman Coulter) using a 25% w/v sucrose cushion ...
-
bioRxiv - Immunology 2020Quote: ... a lyse-wash protocol (Optilyse B, Beckman-Coulter) was performed ...
-
bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
-
bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
-
bioRxiv - Cell Biology 2021Quote: ... The post-nuclear supernatants were ultracentrifuged using SW41Ti rotor at 100,000 g for 3 h at 4 °C on Optima L-90K (Beckman Coulter). The fraction between 0.4–0.5 M sucrose was collected ...
-
ACE2-lentiviral transduction enables mouse SARS-CoV-2 infection and mapping of receptor interactionsbioRxiv - Microbiology 2021Quote: ... UV-inactive SARS-CoV-2 was then partially-purified using a 20% sucrose cushion centrifuged at 175,000 x g for 3-4 hr using SW32Ti rotor (Beckman Coulter). The sample that has passed through the sucrose was collected and concentrated using Amicon Ultra-15 Centrifugal Filter Units with 100 kDa cutoff (Merck Millipore) ...
-
bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 4°C for 3 h at 100,000 g by using Avanti J-30XP (Beckman, USA), and the fractions were collected using a Brandel Fractionation System (USA ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The gradients were ultra-centrifuged at 41,000 rpm for 3 hours at 4°C (Optima L-80 XP ultracentrifuge (Beckman-Coulter)) ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
-
bioRxiv - Molecular Biology 2023Quote: 8 mL of viral supernatant on a 3 mL of 20% sucrose cushion were ultracentrifugated for 3 h at 100,000 x g at 4°C using an Optima L80-XP instrument (Beckman Coulter). After ultracentrifugation ...
-
bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
-
bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gradients were ultra-centrifuged for 3 h at 175,000g at 4°C in a SW41Ti rotor (Beckman-Coulter, USA) and analyzed by measuring the absorbance continuously at 260 nm using a piston gradient fractionator (Biocomp Instruments) ...
-
bioRxiv - Molecular Biology 2022Quote: ... and spun at 40,000 rpm for 3 hours at 4°C using a SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter). Sucrose gradient fractionation was performed by using a Brandel Density Gradient Fractionation System ...
-
bioRxiv - Biochemistry 2023Quote: ... The sucrose density gradient was centrifuged at 50,000 rpm for 3 hours at 4°C in a TLS-55 swinging bucket rotor (Beckman Coulter) with minimum acceleration up to 5,000 RPM and deceleration with no break ...
-
bioRxiv - Bioengineering 2023Quote: ... For performing cryogenic transmission electron microscopic imaging EVs were centrifuged and washed in PBS at 100,000 x g for 3 hours at 4°C in a SW41 Ti rotor (Beckman Coulter).
-
bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
-
bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
-
bioRxiv - Molecular Biology 2024Quote: ... The gradient was centrifuged at 54,000 rpm for 3 h at 4 °C using a SW55 Ti rotor (Beckman Coulter). After centrifugation ...
-
bioRxiv - Biochemistry 2024Quote: ... Equivalent amounts of cell lysates prepared as described above were loaded onto the sucrose gradients and spun at 35,000 g for 3 hours at 4°C in a SW41 rotor (Beckman Coulter). After centrifugation the polysomes were fractionated and absorbance was monitored at 254 nm for RNA and 680 nm for GFP using the TriaxTM Flowcell (BioComp Instruments) ...
-
bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
-
bioRxiv - Neuroscience 2020Quote: ... and were centrifuged at 82,500 x g for 2 h at 4 °C (Beckman SW28 swing rotor). The band between 1.0 and 1.2 M sucrose was collected as the synaptosome fraction and diluted with 80 mM Tris-HCl (pH 8.0) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... in 67 mM HEPES-KOH pH 7.2) after 2 h of ultracentrifugation (4 °C, 5000 RPM, Beckman SW41 ...
-
bioRxiv - Cancer Biology 2024Quote: ... centrifuged twice at 100,000 × g for 2 h at 4°C (Beckman Coulter Optima XE-90 Ultracentrifuge). The supernatant was replaced with DPBS between ultracentrifugation spins ...
-
bioRxiv - Molecular Biology 2023Quote: ... followed by ultracentrifugation at 70k RPM at 4⁰C for 2 h (Optima TLX ultracentrifuge, Beckman Coulter). Ribosome pellets were then resuspended in Trizol and extracted for RNA according to manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... at 28 K rpm for 2 h at 4°C in an SW32 swing-bucket rotor (Beckman). SG3ΔEnv virions were thoroughly resuspended using 500 μl Hepes/Salt buffer per tube ...
-
bioRxiv - Genomics 2024Quote: ... the virus was ultra-centrifuged at 24,000 rpm at 4 C° for 2 h using SW32Ti (Beckman) and the virus pellets were resuspended in RPMI media with 10% FBS to result in a 20-fold concentrated virus and stored at −80 C°.
-
bioRxiv - Microbiology 2020Quote: ... The supernatant was ultracentrifuged at 120,000 g for 3 h at 4°C using an MLS-50 rotor (Beckman Coulter, USA) to collect 120k pellet vesicles ...
-
bioRxiv - Biophysics 2021Quote: The aggregation products formed in Eppendorf tubes under agitation conditions at pH 3 and pH 4 were centrifuged using an Optima MAX-XP ultracentrifuge (Beckman Coulter) in a TLA-55 rotor at 40,000 rpm at 20°C for 45 min ...
-
bioRxiv - Microbiology 2024Quote: ... and resolved by ultracentrifugation at 36,000 rpm for 3 h at 4°C with the fastest braking in Optima XPN-80 Ultracentrifuge equipped with SW-41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 mL/fraction ...
-
bioRxiv - Immunology 2020Quote: ... CD103 (APC, B-Ly7, eBioscience, or biotin, 2G5, Beckman Coulter ...
-
bioRxiv - Immunology 2024Quote: ... and DuraClone IM B cell tubes (#B53318, Beckman Coulter) respectively ...
-
bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
-
bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
-
bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
-
bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
-
bioRxiv - Biophysics 2024Quote: ... The virus was pelleted from supernatants via centrifugation at 14000 rpm for 6 hours at 4°C using a Beckman J2-21 high-speed ultracentrifuge fitted with a JA14 rotor (Beckman Coulter Inc., Fullerton, CA, USA). After carefully removing supernatants ...
-
bioRxiv - Plant Biology 2021Quote: ... sucrose was transferred to the bottom of the tube using a Pasteur pipette and centrifuged at 27,000 rpm for 3 hours at 4° C using an SW28 Ti rotor (Beckman Coulter, Brea, CA). Each pellet containing the virus particles was resuspended in 1 mL resuspension buffer overnight with gentle shaking at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... and centrifuged at 16000 ×g for 3 hours at 4 °C in an Avanti J-25 centrifuge (Beckman Coulter Life Sciences, USA) with a JA-25.50 fixed-angle aluminum rotor (Beckman Coulter Life Sciences ...
-
bioRxiv - Molecular Biology 2021Quote: ... sucrose gradients prepared with lysis buffer without Triton X100 and centrifuged at 32,000 rpm for 3 hrs at 4 °C in an SW41Ti rotor (Beckman Coulter, Cat:331362). The sucrose gradients were fractionated using BR-188 Density Gradient Fractionation System with 60% (m/v ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...