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Citations for Beckman :
1 - 50 of 1537 citations for tert Butyl 2 chloro 5H pyrrolo 3 4 b pyridine 6 7H carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Immunology 2021Quote: ... CD38 APC-AF700 (clone LS198-4-3; Beckman Coulter), CD19 APC-Cy7 (clone SJ25C1 ...
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bioRxiv - Cell Biology 2021Quote: ... The population of Tert+:Ki67+ was assessed by FACS (Gallios™ 561, Beckman Coulter).
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bioRxiv - Neuroscience 2021Quote: ... KG, Staufen, Germany) (position 6, 4 × 5-s bursts) and subsequently centrifuged at 48,000g at 4°C (Beckman Avanti J-251 Ultracentrifuge ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 °C for 3 h (SW40 Ti rotor, Beckman-Coulter, Germany), the supernatant was discarded ...
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bioRxiv - Immunology 2023Quote: ... AlexaFluor 700 anti-human CD38 (clone LS198-4-3, Beckman Coulter), APC-Cy7 anti-human CD19 (clone SJ25C1 ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Bioengineering 2024Quote: ... for 2 hrs at 4°C in polypropylene tubes (Beckman Coulter). Concentrated Cas9-EDVs were resuspended in Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by centrifugation at 36,000 rpm for 3 hours at 4°C (Beckman Coulter). RNA was then extracted from the monosomes fraction using Trizol (Invitrogen ...
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bioRxiv - Genomics 2020Quote: ... and separated by ultracentrifugation at 4°C for 3 hours (35300 rpm, Beckman, SW41 rotor) through a 5-50% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Biophysics 2023Quote: ... precipitated by ultracentrifugation (35,000 rpm for 3 h at 4 ° C, Beckman-Coulter L90K, USA), and then redissolved in TNE/5 buffer.
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bioRxiv - Immunology 2024Quote: ... at 100,000 × g for 3 hours and at 4 °C (Type 45 Ti, Beckman Coulter) to concentrate the exosomes ...
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bioRxiv - Cancer Biology 2024Quote: ... at 100,000 × g for 3 hours at 4°C (345776, Type 45 Ti, Beckman Coulter). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Biophysics 2020Quote: ... After centrifugation at 30,000 rpm (4 ° C for 3 h) in the SW45Ti rotor (Beckman Instruments), eight 1.5-ml fractions were collected from the top of a gradient ...
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bioRxiv - Biochemistry 2020Quote: ... Gradients were resolved at 36,000 rpm for 3 hours at 4 °C in SW41 rotor (Beckman). Both separation and fractionation of gradients used a Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Biochemistry 2020Quote: ... subjected to centrifugation (36,000 rpm for 3 hours at 4 °C, SW-41Ti Beckman Coulter rotor) and analysed using Biocomp Gradient Station (BioComp Instruments ...
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bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were centrifuged at 35,000 rpm for 3 hours at 4°C (SW41 Ti rotor; Beckman) and subsequently fractionated using a Piston Gradient Fractionator (Biocomp instruments ...
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bioRxiv - Biophysics 2023Quote: ... iVCL supernatant was centrifugated for 3 h at 25000 rpm and 4 °C (Beckman-Coulter L90K, USA). Precipitate was resuspended in the TNE buffer (0.1 M Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... followed by ultracentrifugation at 4°C for 2 h (38000 rpm, Beckman, SW41 rotor) through a 20-60% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were seeded into 6-well plates at a concentration of 4×105 cells per well and counted cumulatively with a Coulter counter (Beckman) every three days for two weeks ...
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bioRxiv - Cell Biology 2021Quote: ... Tert+ acinar cells were sorted into Tert+ and Tertcells among live cells based on the gate and collected by FACS (MoFlo®Astrios™, Beckman Coulter). C57BL/6J mice were used as negative controls for gating ...
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Rhes protein transits from neuron to neuron and facilitates mutant huntingtin spreading in the brainbioRxiv - Neuroscience 2021Quote: ... The gradient was spun for 3 h at 180,000 × g (average) at 4°C (SW41 Beckman ultra-centrifuge). After the spin the top of the gradient was removed and discarded ...
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bioRxiv - Microbiology 2021Quote: ... The outer membrane vesicles (OMVs) were then isolated by ultracentrifugation (140 000xg, 4°C) for 3 hours (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were ultracentrifuged at 28.000g for 3 h at 4°C in a SW 28 Ti rotor (Beckman). 1.13 mL fractions were then collected using the Gradient Station model 153 ...
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bioRxiv - Biochemistry 2023Quote: ... Gradients were centrifuged at 35K rpm for 3 hrs at 4°C in a SW-41Ti rotor (Beckman) with maximum acceleration and no brake using a Beckman Optima L-90 Ultracentrifuge ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by ultracentrifugation at 120,000 × g at 4 °C for 2 hours (Beckman, TLA120.4 rotor). The resulting pellets were washed in cold PBS and underwent a second ultracentrifugation at 120,000 × g at 4 °C for 2 hours ...
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bioRxiv - Biochemistry 2020Quote: ... One was through twice daily measurement of 2 or 3 levels of QC specimens obtained from Beckman Coulter Inc ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...