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Citations for Beckman :
51 - 100 of 417 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The supernatants were collected and purified through 15-45% (w/v) sucrose density gradients by ultracentrifugation at 151,000 x g (using 17 mL tubes in a Beckman SW32.1 Ti rotor) for 3 h at 10°C21 ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Microbiology 2022Quote: ... The clarified supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and pelleted through a 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2024Quote: ... The clarified supernatants were collected and pelleted through 30% (w/v) sucrose cushions at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 h at 10°C ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
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bioRxiv - Microbiology 2021Quote: ... VLPs were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion using a Beckman SW32Ti rotor (Beckman-Coulter) at 20,200 rpm for 2h at 4°C.
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bioRxiv - Cell Biology 2020Quote: ... and layered onto a continuous 10-50 w/w % sucrose gradient prepared in a 2.2 ml ultra-clear tube (11×34 mm, Beckman Coulter) using a two-step program in Gradient Master 108 machine ...
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Microbiology 2024Quote: ... Clarified supernatant was loaded onto a 30% sucrose cushion (30% w/w sucrose in TMN buffer) and pelleted at 39000g for 30min (rotor 70Ti Beckman). Pellet was resuspended in 2ml of TMN buffer ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified using 1.5x v/v AMPure XP (Beckman Coulter) with 80% ethanol washes and eluted into 25 μL of water for downstream processing ...
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bioRxiv - Immunology 2024Quote: ... PCR products were purified using 0.7x v/v AMPure XP (Beckman Coulter) with 80% ethanol washes and eluted into 10 μL of water for downstream processing ...
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bioRxiv - Neuroscience 2024Quote: ... and cleaned up using 1.8X (v/v) SPRIselect beads (B23318, Beckman Coulter). Adaptor-ligated DNA fragments were amplified using NEBNext Unique Dual Index Primer Pairs (E6441A ...
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bioRxiv - Neuroscience 2024Quote: ... and cleaned up using 1.75X (v/v) SPRIselect beads (B23318, Beckman Coulter). Adaptor-ligated DNA fragments were amplified using NEBNext Unique Dual Index Primer Pairs (New England BioLabs ...
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bioRxiv - Microbiology 2021Quote: ... The remaining lysate was layered on top of a 30% (w/w) sucrose cushion and centrifuged using a Beckman SW41 rotor (Beckman Coulter) at 32,000rpm and 4°C for 1 h ...
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bioRxiv - Biophysics 2024Quote: ... and particles from media were concentrated by ultracentrifugation through a 20% (w/w) sucrose cushion for 90 min at 27,000 rpm (at 4ºC) in a Beckman SW32 rotor (Beckman Coulter Life Sciences). Particles were resuspended either in PBS or in PBS containing 10% FBS and 10 mM HEPES (pH 7.2) ...
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bioRxiv - Synthetic Biology 2024Quote: ... mixed with a 180% (v/v) ratio of AMPure XP beads (Beckman, US), cleaned up with two consecutive steps of 80% EtOH ...
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bioRxiv - Genomics 2020Quote: ... Libraries were size selected using 0.7x-0.9x (v/v) AMpure XP beads (Beckman Coulter) to final length of 200-500 bp and sequenced by NextSeq 500 using 60 sequencing cycles for Read 1 and 15 cycles for Read 2.
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bioRxiv - Cell Biology 2023Quote: ... The resulting DNA was purified using 1x v/v AMPure XP (Beckman Coulter, A63881) and annealed at 50 °C for 1 hour in the presence of 50 mM NaCl and 100 nM splint oligo ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Cell Biology 2020Quote: ... 30% Percoll (v/v) (pH 7.4)) by ultracentrifugation (SW55-Ti rotor, Optima-XE100 ultracentrifuge, Beckman Coulter) at 95,000g for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... DNA was purified and concentrated using 1X v/v SPRI Select beads (Beckman Coulter, Cat # B23317) and eluted in 50 μL of water according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... after mixing the sample with a 180 %(v/v) ratio of AMPure XP beads (Beckman, US). Next ...
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bioRxiv - Genomics 2024Quote: ... DNA samples were incubated with AMPure XP beads (Beckman Coulter, A63880, dilute to 35% v/v) at a beads to sample ratio of 3.5:1 to retrieve large fragments (greater than 3kb) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EGTA and 30% Percoll (v/v)) in 14 × 89-mm centrifuge tubes (cat. 344059, Beckman). The tubes were then centrifuged on a SW 41 Ti rotor (Beckman ...
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bioRxiv - Plant Biology 2023Quote: ... and 2 mL of 5% solution in a 14 x 89 mm (13.2 mL) thin walled ultra-clear centrifuge tubes (Beckman Coulter, Cat. # 344059). Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor ...
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bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
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bioRxiv - Biochemistry 2021Quote: ... 1x BRB80 solution supplemented with 60 % v/v glycerol and 0.5 mM GMPCPP in a 230 μL thick-walled centrifuge tube (Beckman-Coulter). Care was taken to maintain the two distinct liquid phases ...
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bioRxiv - Cancer Biology 2020Quote: ... cellular nucleic acids from each lysed single cell were extracted from TCL lysis buffer using a 2.2x (v/v) RNA SPRI (RNA-clean AMPure beads, Beckman-Coulter). After ...
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bioRxiv - Bioengineering 2020Quote: ... microbubbles rested for 10 min before the suspension was mixed and diluted to 0.2% v/v in Isoton (Beckman-Coulter). For each vial ...
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bioRxiv - Systems Biology 2024Quote: ... 0.14 vol Mg-PEG buffer (70 mM MgCl2 in 0.7% (v/v) Polyethylene glycol 8000) and washed AMPure XP beads (Beckman Coulter) for 60 minutes while shaking to keep the beads in suspension ...
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bioRxiv - Cell Biology 2023Quote: ... 30% Percoll (v/v)] followed by 3 mL of MRB in a 13 mL ultracentrifuge tube (Beckman Instruments, Inc, CA 331372) and spun at 95,000 x g for 45 min in a TH641 rotor ...
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bioRxiv - Microbiology 2024Quote: ... 1.5 ml of 95% (v/v) n-butanol in concentrated HCl was added and then spun at 2,000 x RPM (Beckman Coulter SX4250) for 5 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... 2.5 ml of each solution were used to form the gradient in a 14 ml Ultra-Clear™ centrifuge tube (Beckman Coulter, Brea, CA, USA), with the highest sucrose concentration at the bottom of the tube ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Microbiology 2023Quote: ... or Kaluza v.2.1 (Beckman Coulter, Brea, CA, USA). Gating strategies and representative raw data are shown in the Supplement.
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bioRxiv - Cancer Biology 2023Quote: ... We then purified the pooled samples with a 1.2 v/v ratio of the sample and Agencourt Ampure XP bead suspension (Beckman Coulter, cat. no. A63881). After purification ...
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bioRxiv - Cell Biology 2023Quote: ... either the dye was solubilized with 10% (v/v) acetic acid and the absorbance was measured at 570 nm in a spectrophotometer microplate reader (Beckman Coulter, Model AD340), or alternatively ...
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bioRxiv - Cancer Biology 2022Quote: ... using CytExpert software v 2.4.0.28 (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Molecular Biology 2021Quote: ... Beads were immobilized on a magnetic rack and the supernatant containing the desired DNA to be sequenced was taken and purified using 1X v/v SPRI Select magnetic beads (Beckman Coulter, Brea, CA, Cat # B23317) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2020Quote: ... 150 V (DelsaMax PRO Light Scattering Analyzer, Beckman Coulter, United States) in distilled water ...
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bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
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bioRxiv - Cell Biology 2024Quote: ... Excess teltail ss-adaptor and short bulk genomic DNA fragments were removed by DNA size selection using 25 μl SPRI select (Beckman, 0.5x v/v SPRI beads to DNA suspension). After 2 washes with 80% EtOH according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Apoptosis was analyzed using Annexin V-FITC / 7-AAD kit (Beckman Coulter) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... using a CytoFLEX cytometer with the CytExpert software (v 2.3.1.22, Beckman Coulter). Sufficient washing was confirmed by absence of the anti-CD19-FITC signal (data not shown).
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bioRxiv - Synthetic Biology 2024Quote: ... Data acquisition was performed using the Kaluza software (Beckman Coulter, v.1.1.20388.18228) for flow cytometry and the LE-SH800SZFCPL Cell Sorter software (Sony ...
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bioRxiv - Pathology 2020Quote: ... 50 μl of Truecount bead solution (Beckman Coulter) was added to each sample ...