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Citations for Beckman :
1 - 50 of 417 citations for Alpha Cyclodextrin Solution 5% w v since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%–45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Biophysics 2021Quote: ... solubilized supernatant was collected and subjected to sucrose gradient ultracentrifugation (10% to 50% (w/v) sucrose in buffer A with 0.03% (w/v) digitonin) at 35000 rpm (sw41 Ti rotor, Beckman Coulter) for 16 hours at 4℃ ...
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bioRxiv - Molecular Biology 2023Quote: ... Dialyzed nucleosomes were purified in a 5-30% w/v sucrose gradient with a SW41 Ti (Beckman Coulter) rotor in an Optima L-90K ultracentrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Cell Biology 2022Quote: ... The stock solution of aggregate-depleted BSA (5% w/v) was prepared by overnight centrifugation at 100,000 x g (SW28 rotor Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Clarified supernatants were layered over 2 ml of a 25% (w/v) sucrose solution in 13.2 ml Thinwall Polypropylene SW41 Ti ultracentrifuge tubes (Beckman-Coulter, Brea, USA) and made up to a total volume of 10 ml with PBS without calcium chloride or magnesium chloride (PBS[-]) ...
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bioRxiv - Immunology 2021Quote: ... were loaded onto 5-25% (w/v) linear sucrose gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 3 h 15°C ...
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bioRxiv - Cell Biology 2023Quote: ... continuous 15-45 % (w/v) sucrose gradients were prepared in SW41 tubes (Beckman) in Polysome Gradient Buffer (10 mM Hepes-KOH ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 50% (w/v) sucrose) was filled into ultracentrifuge tubes (Polypropylene 10 ml Tube, Beckman Coulter), the sheared grappling hooks were gently layered on top ...
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bioRxiv - Microbiology 2022Quote: ... Samples fixed in formaldehyde solution were diluted to 1% (v/v) in filtered IsoFlow Sheath Fluid (Beckman). Of this ...
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bioRxiv - Bioengineering 2022Quote: ... then subjected to 25-60% (w/v) sucrose gradient in SW41 centrifuge tube (Beckman, Brea, CA, USA) for centrifugation at 28,000 rpm for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... followed by sedimentation through a discontinuous 20-45% (w/v) sucrose at SW41 Ti rotor (Beckman Coulter) for 12 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... 300 µL of 35% sucrose (w/v) were added to a 600 µL ultracentrifuge tube (Beckman-Coulter) and the resuspended pellet fraction in 45% sucrose (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Microbiology 2024Quote: ... Precipitate was pelleted though a 30% (w/v) sucrose cushion at 120,000 × g (using a Beckman SW32 Ti rotor) for 3.5 h at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... α -DDM] for negative staining or maltose gradient for cryo-EM data collection [0.1–1.4 M maltose with 25 mM MES-NaOH (pH 6.5) and 0.03% (w/v) α-DDM at 154,300 xg (SW 41 Ti rotor, Beckman Coulter) for 24 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting cell extracts were layered over a 20% sucrose cushion (polysome buffer + 20% w/v sucrose) and ultracentrifuged (Beckman Coulter TLA100.3 Fixed-Angle Rotor ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Immunology 2024Quote: ... and nanocontainers were then further purified by sucrose density ultracentrifugation (10-40% w/v gradients prepared in 50 mM potassium phosphate buffer at pH 7.5) in a SW-32 rotor (Beckman Coulter) spinning at 28,000 rpm for 4 hours at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Particles were then concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion at 28,000 rpm using a Beckman TLA-100 fixed angle-rotor (Beckman Coulter) for 90 min or for the 6-well format at 44,000 rpm using a Beckman TLA-55 fixed angle-rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2024Quote: ... The precleared media was then layered dropwise onto a 30% (w/v) sucrose cushion in an ultracentrifuge tube (Beckman Coulter). Ultracentrifugation was done at 134,000xg for 4h at 4°C using an SW 41 Ti rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... Cytoplasmic lysates were loaded onto a 18-50% (w/v) sucrose gradient and ultra-centrifuged in an SW40Ti rotor (Beckman) for 4:15 hours at 170,920 × g at 4°C and fractionated as above.
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bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 ml of each lysate was mixed with 2 ml of 1% sarkosyl and then layered on 2 ml of 150% (w/v) cesium chloride (in 1X TE, pH 7.5) in 4.9 ml OptiSeal tubes (Beckman Coulter, cat #362185). Samples were centrifuged for 18 h at 42,000 rpm in an NVT 65.2 rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... The assembled complexes were incubated for 5 min at 30 °C and for 10 min on ice before they were loaded on a 30% (w/v) sucrose cushion in TLA-100 tubes (Beckman Coulter). The sucrose cushions were centrifuged for 2 h at 390,880 xg and 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The proteoliposomes were mixed with 60% (w/v) sucrose and loaded at the bottom of a 10 ml polycarbonate centrifuge tube (Beckman Coulter). A discontinuous sucrose gradient was prepared (20% ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were solubilised in 150 μL of cold TNE buffer and a volume of 1.5 ml was laid on a five step 20 - 60% (w/v) sucrose gradient prepared in Ultra-Clear 13 ml tubes (Beckman Coulter). The tubes were centrifuged at 35,000 rpm for 2h at 4°C in a Beckman SW41 rotor ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 was subsequently removed from the mitoribosomes via a 10 – 40 % (w/v) sucrose gradient in 1x MB (17 h, 22500 rpm, SW 32 Ti rotor (Beckman Coulter), 4 °C) ...
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bioRxiv - Biophysics 2023Quote: ... and concentrated by several washing steps in FBS-free Opti-MEM® and virus particles were concentrated by ultracentrifugation on a 60% (w/V) iodixanol cushion at 21°C (131,500 x g, 90 min, SW28 rotor, Beckman Coulter, Ireland) to a final volume of 1 mL ...
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bioRxiv - Biochemistry 2023Quote: ... and 400 μL was loaded onto a 5-30% (w/v) buffered sucrose gradient (in Buffer B) in a 14 mm × 89 mm thin-wall Ultra-Clear tube (Beckman # 344059) that was formed using a Biocomp Gradient Master ...
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bioRxiv - Bioengineering 2022Quote: ... Supernatant (33 mL) was placed on a 3 mL 25% (w/v) sucrose pad in SW28 tube (Beckman, Brea, CA, USA) and centrifuged at 24,000 rpm ...
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered by 0.22 μm nylon membrane followed by centrifugation through a cushion of 50 % (w/v) sucrose with 36,000 rpm at 4 °C for 4 h (Beckman SW41 Ti rotor). The pellet was resuspended with PBS and the copy number of DWV was quantified by qRT-PCR.
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was collected and virus pelleted through 30% (w/v) sucrose cushion at 151,000 × g (using a Beckman SW 32 Ti rotor) for 3.5 hours at 10°C ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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bioRxiv - Microbiology 2024Quote: ... The supernatants were collected and purified through 15-45% (w/v) sucrose density gradients by ultracentrifugation at 151,000 x g (using 17 mL tubes in a Beckman SW32.1 Ti rotor) for 3 h at 10°C21 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The pellets were resuspended in TEMB buffer and then loaded onto sucrose gradients (10−50%, w/v) followed by ultracentrifugation (Beckman, XL100K ultracentrifuge) at 105,000 g for 30 minutes ...