Labshake search
Citations for Beckman :
901 - 950 of 970 citations for N N Dimethyl N 2 methacryloyl ethyl N 1 octyl ammonium iodide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Inhibition of Ribosome Biogenesis in vivo Causes p53-Dependent Death and p53-Independent DysfunctionbioRxiv - Cell Biology 2024Quote: ... 1 mL of the lysates was loaded onto a 10%-50% sucrose gradient in a 14 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a Beckman Optima LE-80K Ultracentrifuge Floor Centrifuge at 261,000 × g for 135 minutes at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Systems Biology 2020Quote: ... Next the cultures were diluted 1:40 with 0.5M NaCl and immediately measured in Multisizer™4 COULTER COUNTER® (Beckman Coulter) (Coulter ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) and AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). DNA content and quality of indexed libraries were examined with a Qubit 4.0 using the high-sensitivity kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume:library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants were transferred into SW60 tubes (in vivo studies 1–4) or SW40 tubes (donated patients) (Beckman Coulter Inc., Brea, USA) and centrifuged at 10,000xg for 35 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... The PNS- Nycodenz gradient was centrifuged for 1 h at 141,000 x g at 4°C (46,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... 670 µL of lysate was slowly dispensed on top of 330 µL of a 70% sucrose cushion in a 1 mL polycarbonate tube (Beckman Coulter, 343778) and centrifuged in a MLA-130 ultracentrifuge rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... The top agar was then scraped off the LB agar and centrifuged for 26,000 x g at 4°C for 1 hour (Beckman Coulter JA-17). The supernatant was then removed ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 931 x g [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were mixed with polybrene and increasing amounts of the lentiviral CP1985 sgRNA library and plated in 1 mL aliquots onto 12 well plates and centrifuged at 931 x g [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A ...
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bioRxiv - Microbiology 2024Quote: ... Crude ribosomes were separated from other low molecular weight cellular contents by centrifugation at 35000 rpm (126,000g) for 1 hour at 4 °C (Beckman rotor Type 70Ti). The crude ribosome pellet was resuspended in 10 mL HMA-10 buffer and layered on top of a 10 mL 32% sucrose solution in HMA-10 buffer and centrifuged for 16 hours at 37,000 rpm (140,000g ...
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bioRxiv - Microbiology 2024Quote: Samples (1 mL) from the mother culture and post treatment culture growth were analysed using a CytoFLEX LX flow cytometer (Beckman Coulter, USA) to compare difference in bacterial contamination ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was segregated subsequent to centrifuging the brain tissue homogenate for 1 h at 100,000g and 4°C utilising an ultra-high-speed centrifuge (Beckman, Optima MAX-XP). The tissue residue was then reprocessed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then 0.1 μL of library compound (1 mM) dissolved in DMSO was added using pintool integrated with Beckman NX (Beckman Coulter, Brea, CA). The final compound concentration was 20 μM and the final DMSO concentration was 2% ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) Productively-infected cells were identified based on p24 and CD4 detection as described above ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of infected cells (p24+ ...
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bioRxiv - Genomics 2024Quote: Cells from frozen stocks were diluted 1:30 into 300 µl of LB in a deep 96-well plate (Beckman Coulter, #267007), covered with a breathable film ...
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bioRxiv - Biophysics 2024Quote: ... Resulting libraries were pooled (with the Mosquito) and purified using AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume (Beckman Coulter, Brea, CA, USA). Library yield and quality were determined by TapeStation electrophoresis with an HSD1000 ScreenTape system (Agilent Technologies).
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bioRxiv - Bioengineering 2024Quote: ... a suspension of the microparticles was prepared in double deionized water and sized using a laser diffraction method to measure the size distribution of microparticles (Supplementary Figure 1, Coulter LS230, fitted with the hazardous fluids module, Beckman Coulter, UK) while under agitation to prevent the particles settling ...
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bioRxiv - Genetics 2024Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean Ampure XP beads (Beckman Coulter, A63987).
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Biochemistry 2020Quote: ... The labeled and unlabeled polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, Beckman TLA-55 rotor). The pellets were rinsed once with 1 mL G-buffer and re-suspended into ice cold 0.5 mL G-buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Microbiology 2020Quote: ... samples absorbance at 485 nm was measured in a 1 cm quartz cuvette using a DUTM 730 spectrophotometer (Beckman Coulter, Brea, CA, USA). 155 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 M sucrose in HK was added to the reaction to a final concentration of 1 M (“Input” sample) and transferred to 7 × 20 mm polycarbonate centrifuge tubes (Beckman Coulter cat #343775). HK buffer with 0.75 M sucrose was gently laid on top of the reaction mixture followed by a layer of HK ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Sequencing libraries for each replicate were generated via qPCR and sequencing libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume: library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Biochemistry 2022Quote: ... The polymerized actin samples were pelleted down by ultracentrifugation at room temperature (45000 rpm for 1 hour, TLA-55 rotor, Beckman Coulter, Indianapolis, IN). The pellets were rinsed once with a 1 mL G-buffer and re-suspended into ice-cold 0.5 mL G-buffer ...