Labshake search
Citations for Beckman :
801 - 850 of 970 citations for N N Dimethyl N 2 methacryloyl ethyl N 1 octyl ammonium iodide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
-
bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
-
bioRxiv - Microbiology 2022Quote: ... PCR products were confirmed through visualization in 1% agarose gel and purified using AMPure XP beads (Beckman Coulter, Indianapolis, U.S.A.). PCR libraries were pooled together and sent to the Centre d’expertise et de services Genome Québec (Montréal ...
-
bioRxiv - Cell Biology 2022Quote: ... lipoprotein particles (LPPs) were purified from the non-sedimentable fraction after 1 h of ultracentrifugation in a Ti-45 rotor (Beckman) at 44,000 rpm at 4 °C ...
-
bioRxiv - Genetics 2022Quote: ... followed by 72°C for 1 minute followed by purification with 1X Agencourt AMPure XP beads as per the manufacturer’s instructions (Beckman Coulter).
-
bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
-
bioRxiv - Molecular Biology 2022Quote: ... and centrifuged at 15 000 RPM for 1 minute using an AllegraTM X-22R Centriuge (Beckman Coulter™, California, USA) to ensure all reagents were concentrated at the bottom of the wells ...
-
bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
-
bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
-
bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
-
bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
-
bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
-
bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
-
bioRxiv - Synthetic Biology 2024Quote: ... then approximately 1/10th of the volume of each pool was purified with a 0.9:1 ratio of AMPure XP beads:PCR mix (Beckman Coulter, A63881). The complete volume of the AMPure-cleaned 1st round PCR products for each library was used as the templates for a “2nd round PCR,” which added the remainder of the TruSeq HT i5 and i7 Illumina adaptor sequences ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
-
bioRxiv - Cell Biology 2024Quote: ... The discontinuous gradient was spun for 18 hours at 100,000xg and collected into twelve 1 mL fractions (SW40 Ti rotor, Beckman Coulter). SEVs are typically located in fractions 6 and 7 ...
-
bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution).
-
bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
-
bioRxiv - Immunology 2024Quote: ... 1 million peripheral immune cells were pipetted into the DuraClone immunophenotyping tube (DuraClone IP Phenotyping kit from Beckman Coulter, USA). Each tube contained anti-CD45-KO ...
-
bioRxiv - Microbiology 2024Quote: ... Intracellular Gag staining was performed in PBS+1%BSA+0.05%Saponine using the KC-57 anti-Gag antibody (Beckman-Coulter) for 30min at 4°C ...
-
bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed two times with PBS supplemented with BSA (1%) and data recorded in a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
-
bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
-
bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
-
bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
-
bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
-
bioRxiv - Microbiology 2024Quote: ... cells were washed in PBS containing 1% BSA and resuspended in PBS containing 0.5% BSA before fluorescence quantification by CytoFlex S Benchtop Flow Cytometer (Beckman Coulter).
-
bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
-
bioRxiv - Genetics 2024Quote: ... An aliquot of each cell suspension was diluted 1:1,000 with seawater and cell count was performed using flow cytometry (CytoFLEX S, Beckman Coulter) set at medium flow (30 µl/min) ...
-
bioRxiv - Biophysics 2024Quote: ... 100 μL of Origami-R1-R2 was placed on top of a quasi-linear glycerol gradient (15–45%, in 1× TE-Mg2+ buffer) in a 0.8 mL Open-Top Thinwall Ultra-Clear tube (Beckman Coulter) and spun in an SW 55 Ti rotor (Beckman Coulter ...
-
bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
-
bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
-
bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
-
Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
-
bioRxiv - Developmental Biology 2020Quote: ... Subsequently libraries were subject to size selection to exclude DNA fragments larger than 1 kb using SPRIselect magnetic beads (Beckman Coulter). Samples were sequenced on a Illumina HiSeq 4000 platform to obtain 50 bp paired-end reads ...
-
bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
-
bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and 1 mM TCEP added before use) and centrifuged for 24 h at 28,000 rpm in a 70Ti rotor (Beckman Coulter, Inc.). The pellets were suspended in washing buffer (20 mM Tris-HCl ...
-
bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on Cytoflex (Beckman Coulter, Brea, CA) and analysed with FlowJo v10.0.8 (Tree Star ...
-
bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
-
bioRxiv - Molecular Biology 2020Quote: ... sucrose cushion prepared in PSB with 100 nM CaCl2 and spun for 1 hour at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA) to remove aggregates ...
-
bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
-
bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...
-
bioRxiv - Microbiology 2022Quote: ... and ½ of each plug was inserted into a well of a 1% LE agarose gel (Beckman Instruments, Inc., Palo Alto, CA) and electrophoresed on a CHEF Mapper® XA Pulsed-Field Gel Electrophoresis System (BioRad ...
-
bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR templates were purified using Agencourt AMPure XP (PCR product: AMPure XP beads = 1:0.8; Beckman Coulter, Brea, California, USA) before the second PCR ...
-
bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.