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Citations for Beckman :
851 - 900 of 1472 citations for N Furan 2 ylmethyl 3 bromo 4 fluoro benzamide since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... 36 mL of media per condition was subject to ultracentrifugation at 100,000 x g for 6 hours at 4 °C in a SW-32-Ti swinging bucket rotor (Beckman). The media was decanted and 500 μL PBS was added to the pellet and left to resuspend overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were loaded based on equal total RNA onto a 10%–50% sucrose gradient and subjected to ultracentrifugation (187,813 g for 1.5 hours at 4 °C) using a SW 41 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Bioengineering 2024Quote: ... The virus was then pelleted by centrifugation at 4°C for 45 min at 25,000 rpm in a sw28 rotor (Beckman) and gently suspended in dilution buffer (150 mM NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... which was then centrifuged at 100,000 × g for 18 hours at 4°C (344060, SW 40 Ti, Beckman Coulter) for further purification ...
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bioRxiv - Cell Biology 2024Quote: ... All ultracentrifugation steps will be performed at 4°C using a SW41 Ti rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg) for 16 h at 4 °C in a Ti-45 rotor (Beckman-Coulter). Ribosomes pellets were then resuspended in dissociation buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested at day 3 post-infection by centrifuging at 2,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Insect cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by centrifuging at 5,000xg for 15 mins at 4°C using a JLA 8.1000 rotor (Beckman Coulter). Bacterial cell pellets were frozen in liquid nitrogen for storage or used immediately for protein purification.
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bioRxiv - Cell Biology 2024Quote: ... Lysates were clarified by centrifugation at 20,000 x g for 45 mins at 4°C in a JA25.50 rotor (Beckman Coulter). Clarified lysates were applied to a gravity flow column several times over 2 ml Ni-NTA resin (Sigma ...
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bioRxiv - Synthetic Biology 2021Quote: ... 72C for 2’) and a X0.65 SPRI bead DNA clean up (AMPure XP, Beckman Coulter) a second PCR using a p5 primer (F6 ...
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bioRxiv - Systems Biology 2022Quote: ... Final libraries were size-selected by performing 2-step bead cleaning (SPRIselect, Beckman Coulter B23318) to remove larger DNA fragments and primer dimers and quality was assessed by Bioanalyzer (Agilent 2100 ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-2 RBD+ were sorted with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were analyzed using FlowJo™ v10.8 (BD Biosciences).
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bioRxiv - Genomics 2022Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Microbiology 2022Quote: ... The filtrate was then ultracentrifuged 100,000 g for 2 hours (Optima L-90K, Beckman Coulter). The vesicle pellet was resuspended in 100 μl of sterile PBS.
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bioRxiv - Genomics 2023Quote: ... then ultracentrifuge at 24,000 rpm for 2 h using SW28 swinging bucket rotor (Beckman Coulter). Upon completion ...
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bioRxiv - Genomics 2024Quote: ... Polyadenylated RNA was purified using 2× sample volume of AMPureXP RNA beads (Beckman Coulter, A66514), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 100 μg/ml cycloheximide) and centrifuged for 3 hours at 35,000 rpm in an SW41 rotor in a Beckman L7 ultracentrifuge (Beckman Coulter, Krefeld, Germany). The gradients were simultaneously fractionated on a Density Gradient Fractionation System (#621140007 ...
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bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...
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bioRxiv - Cell Biology 2022Quote: ... The gradient was formed by sequentially adding each 3 ml layer to a 14 × 89 mm ultra-clear centrifuge tube (Beckman Coulter, Inc.), starting with the 40% solution and ending with the 5% solution ...
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bioRxiv - Zoology 2021Quote: ... Food concentration was maintained at 1500 μm3 mL−1 of phytoplankton cells at the outlet of the tank – ad libitum (Rico-Villa et al., 2009) – and controlled twice daily using an electronic particle counter (Multisizer 3, Beckman Coulter, USA) equipped with a 100 μm aperture tube ...
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bioRxiv - Neuroscience 2022Quote: ... and a 200-μl aliquot of the final supernatant was added to 3 mL of scintillation fluid and the radioactivity was measured by a liquid scintillation counter (Beckman LS 6000SC). Blank values were obtained by performing the reaction in the absence of tissue homogenate and in the presence of boiled homogenate ...
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bioRxiv - Cell Biology 2024Quote: ... conditioned media were subjected to 10 min at 200g for 3 cycles (G146D centrifuge, Gelec®) and 20 min at 2000g (Allegra 64R Centrifuge, Beckman Coulter) to remove cellular debris and apoptotic bodies respectively ...
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bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
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bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...
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bioRxiv - Neuroscience 2021Quote: ... PMCA products were centrifuged at 45,000 rpm for 2.5 h at 4 °C (Optima™ Max Ultracentrifuge, Beckman Coulter, USA), the supernatants discarded and the pellets resuspended in an equal volume of PBS.
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions were subjected to SDS-PAGE and Coomassie blue staining.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM DTT and centrifuged for 16 h at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). The fractions ...
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bioRxiv - Molecular Biology 2020Quote: ... Gradients were centrifuged at 39 000 rpm for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman– Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Developmental Biology 2020Quote: ... Separation of testis cell populations was performed by centrifugal elutriation using a JE-5.0 elutriation system and a 4-ml standard elutriation chamber (Beckman Coulter). The assembly of the system followed the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2020Quote: ... the EV pellets were resuspended in 3 ml 0.32 M sucrose (diluted in PBS) solution and centrifuged again at 100,000xg for 60 minutes at 4°C (SW60 rotor, Beckman Coulter). Sucrose supernatant was removed and pellet was resuspended in 500 μl phosphate-buffered saline containing 0.01% bovine serum albumin (0.2 μm filtered ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was continued for 24 h before harvesting cells by centrifugation (4°C, 8000 rpm, 10 min in Beckman Coulter Avanti JXN-26 centrifuge ...