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Citations for Beckman :
701 - 750 of 1314 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and BA.5 RBD were sorted using the MoFlo Astrios EQ Cell Sorter (Beckman Coulter). The data from FACS were gathered using Summit 6.0 and analyzed with FlowJo software version 10.8.
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bioRxiv - Microbiology 2020Quote: ... Centrifugation was performed at 120,000 × g for 2 hr (Beckman TLS 55 rotor), after which the interface between 0.25 M and 1.1 M sucrose was collected and resuspended in 1 ml 19% OptiPrep for a step gradient containing 0.5 ml 22.5% ...
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bioRxiv - Cell Biology 2022Quote: ... 2× – G2) was interrogated using a using a Gallios flow cytometer (Beckman Coulter) and subsequent data analysis with FlowJo (v.10.8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were centrifuged at 1500 rpm for 2 minutes (Beckman Allegra X15R, 524xg), supernatant was removed and tissue was carefully triturated using fire-polished glass pipettes with 2 mL pre-warmed media ...
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bioRxiv - Synthetic Biology 2021Quote: PCR products were purified with 2 volumes of RNAClean XP beads (Beckman Coulter) and then quality-checked and quantified on a Bioanalyzer (Agilent) ...
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bioRxiv - Immunology 2024Quote: ... and 2 mM DTT) and separated by ultracentrifugation at 148,900xg (Ti55 rotor, Beckman) for 1.5 hours at 4°C ...
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bioRxiv - Genetics 2024Quote: ... DNA was further purified using 2 volumes of Ampure XP beads (Beckman Coulter). DNA was extracted from 10000 undifferentiated or differentiated spermatogonia cell samples by QIAamp DNA Micro Kit (Qiagen # 56304 ...
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bioRxiv - Molecular Biology 2023Quote: ... The ribodepleted RNA was cleaned using 2× RNAClean XP beads (Beckman Coulter A63987), eluting with 20 μL DNase digestion reaction mixture (1.5 μL Turbo DNase (Invitrogen AM2238) ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were diluted 2-fold with PBS and analysed with CytoFLEX S (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: ... 2 mM DTT) with 0.7 M sucrose in Ti-45 tubes (Beckman-Coulter). The tubes were then placed in a Ti-45 rotor and spun at 27,000 rpm (57,000 xg ...
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bioRxiv - Biophysics 2022Quote: ... 2 M urea) by resuspension and centrifugation on a JS-4.2 rotor (Beckman) at 6,000 RPM for 30 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mg/ml Proteinase K) and normal phase 2x AMPureXP (Beckman Coulter, #A63881) bead clean-up ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h using 25 ξ 89 mm polycarbonate tubes (Beckman Coulter, 355618). Pellets were resuspended in 500 μL of sterile phosphate-buffered saline (PBS ...
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bioRxiv - Neuroscience 2024Quote: ... for 2 h using 16 ξ 76 mm polycarbonate tubes (Beckman Coulter, 355603). The resulting pellets were resuspended in 250 μL of sterile PBS ...
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bioRxiv - Biochemistry 2024Quote: ... Step 2 PCR was cleaned up using Agencourt AMPure XP beads (Beckman Coulter), and final DNA concentration was measured using the Invitrogen Quant-IT dsDNA High-Sensitivity HS Assay kit quantified with a BioTek Synergy H1 Multimode Microplate Reader before pooling libraries for sequencing ...
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bioRxiv - Genomics 2024Quote: ... PCR 2 products were purified by DNA AMPure XP beads (Beckman Coulter #A63882) and eluted with 25 uL of water ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Subsequently libraries were subject to size selection to exclude DNA fragments larger than 1 kb using SPRIselect magnetic beads (Beckman Coulter). Samples were sequenced on a Illumina HiSeq 4000 platform to obtain 50 bp paired-end reads ...
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bioRxiv - Developmental Biology 2020Quote: ... incubated with fluorophore-conjugated antibodies for 30min (see Supplementary Table 1) and FACS-sorted using MoFlo Legacy or Astrios cell sorters (Beckman Coulter). Cells were sequentially gated based on size and granularity (forward scatter ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM TCEP added before use) and centrifuged for 24 h at 28,000 rpm in a 70Ti rotor (Beckman Coulter, Inc.). The pellets were suspended in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on Cytoflex (Beckman Coulter, Brea, CA) and analysed with FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... sucrose cushion prepared in PSB with 100 nM CaCl2 and spun for 1 hour at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA) to remove aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...
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bioRxiv - Microbiology 2022Quote: ... and ½ of each plug was inserted into a well of a 1% LE agarose gel (Beckman Instruments, Inc., Palo Alto, CA) and electrophoresed on a CHEF Mapper® XA Pulsed-Field Gel Electrophoresis System (BioRad ...
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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bioRxiv - Microbiology 2022Quote: ... The PCR templates were purified using Agencourt AMPure XP (PCR product: AMPure XP beads = 1:0.8; Beckman Coulter, Brea, California, USA) before the second PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Immunology 2022Quote: ... Samples are washed with 1X FACS buffer again to remove the PFA and finally re-suspended in 1 x FACS buffer for flow cytometry analysis using either Fortessa instrument (Becton Dickinson) or CytoFlex LX instrument (Beckman Coulter) fitted with an automated sampler accommodating 96-well plates ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... Exosome pellets were resuspended in phosphate-buffered saline (PBS) and centrifuged at 27300 rpm for 1 hour (SW60Ti rotor; Beckman Coulter). The exosome pellets were resuspended in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through 40 μm Falcon™ Cell Strainers (Thermo Fischer Scientific, cat no. 08-771-1) and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). Matlab-based software for processing flow cytometry data was developed in-house by Yaron Antebi.
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bioRxiv - Cancer Biology 2021Quote: ... the 197 library compounds dissolved in DMSO or MilliQ (Supplementary Table 1) were added in duplicate using the Echo 550 dispenser (Beckman Coulter). Final drug concentrations ranged standard between 0.1 nM and 10 μM ...
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...