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Citations for Beckman :
651 - 700 of 1314 citations for 2 1 ethylpentyl 1 3 dioxan 5 yl laurate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... then approximately 1/10th of the volume of each pool was purified with a 0.9:1 ratio of AMPure XP beads:PCR mix (Beckman Coulter, A63881). The complete volume of the AMPure-cleaned 1st round PCR products for each library was used as the templates for a “2nd round PCR,” which added the remainder of the TruSeq HT i5 and i7 Illumina adaptor sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Cell Biology 2024Quote: ... The discontinuous gradient was spun for 18 hours at 100,000xg and collected into twelve 1 mL fractions (SW40 Ti rotor, Beckman Coulter). SEVs are typically located in fractions 6 and 7 ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution).
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... 1 million peripheral immune cells were pipetted into the DuraClone immunophenotyping tube (DuraClone IP Phenotyping kit from Beckman Coulter, USA). Each tube contained anti-CD45-KO ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular Gag staining was performed in PBS+1%BSA+0.05%Saponine using the KC-57 anti-Gag antibody (Beckman-Coulter) for 30min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
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bioRxiv - Immunology 2024Quote: ... Cells were then washed two times with PBS supplemented with BSA (1%) and data recorded in a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
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bioRxiv - Immunology 2024Quote: ... and stained for Gag p24 protein with anti-HIV-1 core antigen monoclonal antibody (clone KC57, Beckman Coulter, cat. 6604665).
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bioRxiv - Microbiology 2024Quote: ... cells were washed in PBS containing 1% BSA and resuspended in PBS containing 0.5% BSA before fluorescence quantification by CytoFlex S Benchtop Flow Cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Genetics 2024Quote: ... An aliquot of each cell suspension was diluted 1:1,000 with seawater and cell count was performed using flow cytometry (CytoFLEX S, Beckman Coulter) set at medium flow (30 µl/min) ...
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bioRxiv - Biophysics 2024Quote: ... 100 μL of Origami-R1-R2 was placed on top of a quasi-linear glycerol gradient (15–45%, in 1× TE-Mg2+ buffer) in a 0.8 mL Open-Top Thinwall Ultra-Clear tube (Beckman Coulter) and spun in an SW 55 Ti rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were spun for 3 hours at 4° C and 36,000 RPM (221,632 x g) on SW41-Ti rotor (Beckman Coulter). Monosome fractions were isolated from each sucrose gradient with Brandel Density Gradient Fractionator using 1.5 mL/min flow speed and 60% CsCl ...
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bioRxiv - Microbiology 2022Quote: ... phage suspension was layered on top of a 5–20 % sucrose (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) and centrifuged (Beckman Optima L-K90 ...
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bioRxiv - Microbiology 2022Quote: ... The light-scattering zone was collected and further purified in 20– 70 % sucrose gradient (w/vol, in Tris-HCl pH 7.2 with 3 mM MgCl2 and 10 mM CaCl2) by centrifugation (Beckman Optima L-K90 ...
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bioRxiv - Immunology 2022Quote: ... All experiments in this study were performed using a 3-laser CytoFLEX Flow Cytometer (Beckman Coulter, Brea, CA, USA) and operated using the CytExpert Software v2.4.0.28 (Beckman Coulter) ...
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bioRxiv - Cell Biology 2020Quote: ... The harvested sEVs were resuspended with 3 mL double filtered PBS and centrifuged with MLA-55 rotor (Beckman Coulter) under 100,000 × g at 4 °C for 70 mins to remove non-EV contaminants ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Microbiology 2023Quote: ... and RNA were purified using 3 volume of absolute isopropanol and 1.8 volume of Agencourt RNAClean XP beads (Beckman). RNA was eluted with 6 µL of water ...
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bioRxiv - Plant Biology 2024Quote: ... and centrifuged for 3 h at 260,000 g using an SW 55 Ti swinging-bucket rotor (Beckman Coulter, USA). The final supernatant was discarded ...
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bioRxiv - Biochemistry 2022Quote: ... The membrane was solubilized for 3 hours at 4°C before separation by ultracentrifugation at 100,000 g (Ti45, Beckman) for 45 min ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was centrifuged for 3 h at 115,000 g and 4°C using the XPN-80 ultracentrifuge equipped with a Ti70 rotor (Beckman). EV pellets were resolved in 1 mL ice-cold PBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... 0.01% Sodium Azide in PBS and measured by recording 3 × 104 events using CytoFLEX and Gallios cytometers (Beckman Coulter). The resulting data were analysed using FlowJo v10 (TreeStar) ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20nM of gemcitabine and cells were counted 3 days later using the Z1 Coulter (Beckman).
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (𝑐i ...
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bioRxiv - Microbiology 2024Quote: ... the supernatants were loaded onto 1 mL of 30% sucrose in PBS buffer and centrifuged at 25,000 rpm at 4°C for 3 hours using the 90AT rotor (Beckman). The pelleted viral particles were resuspended in 100 μL of PBS and fixed with formaldehyde (working concentration 0.1% ...
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bioRxiv - Neuroscience 2024Quote: ... Tubes were centrifugated at 100,000 x g (32,500 RPM) for 3 hours at 4°C using a SW55Ti rotor (Beckman Coulter). Synaptosomes enriched in the interface between 1.0-1.25 M sucrose were collected and diluted with ice-cold 0.1 mM CaCl2 ...
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Syntaxin-2 inhibits excessive phagocytic uptake but promotes phagolysosomal clearance in macrophagesbioRxiv - Cell Biology 2024Quote: ... followed by sequential topping with 2 mL each of 35, 25, and 10% sucrose (all prepared in 3 mM imidazole, pH 7.4) in SW40 tubes (344060, Beckman). The samples were then centrifuged at 100,000 g for 1 hr at 4°C using a SW40Ti rotor (Beckman) ...
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bioRxiv - Bioengineering 2024Quote: ... the distribution of microbubble diameter and concentration was assessed using a Coulter counter (Multisizer 3; Beckman Coulter, Fullerton, California). T1 mapping MRI sequences were used to guided sonication targeting ...
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bioRxiv - Cell Biology 2024Quote: ... The 100,000xg SEV-containing pellets were resuspended in 3 ml sterile cold PBS and repelleted at 100,000xg for 4 hrs in a TLA110 rotor (Beckman). SEVs were analyzed for size and number by nanoparticle tracking (ZetaView ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose density gradients (5%–45% w/v) were freshly made in SW41 ultracentrifuge tubes (Beckman) using a Gradient Master (BioComp Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 mM MgCl2) and centrifuged at 45,000 rpm (SW 55 Ti rotor, Beckman Coulter, Inc.) (adapted from Liang et al ...
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bioRxiv - Microbiology 2021Quote: ... Product were PCR amplified by 5 cycles and cleaned up using SPRI beads (Beckman Coulter) and subjected to paired-end 300bp sequencing on Illumina Miseq ...
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bioRxiv - Biochemistry 2023Quote: ... Ultracentrifugation was carried out for 5 hours at 133,000xg on a SW60-Ti (Beckman Coulter) rotor to resolve each gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Biophysics 2024Quote: ... the microtubules were centrifuged for 5 min at 22,000 RPM (TLA100.0, Beckman Optima MAX-XP) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... After 5 days cells were harvested for genomic DNA purification using Agencourt DNAdvance (Beckman Coulter). Transfection of human primary hepatocytes were performed using Lipofectamine MessengerMAX Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...