Labshake search
Citations for Beckman :
651 - 700 of 1143 citations for 6 Chloro 1 2 dihydro 3H indazol 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Samples were 1:1.3 AMPure XP (Beckman Coulter) purified and sent for sequencing ...
-
bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
-
bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
-
bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
-
bioRxiv - Molecular Biology 2019Quote: ... The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter, Fullerton, California, USA). A discontinuous DG was prepared by additional layering of 4 ml of 20% ...
-
bioRxiv - Biochemistry 2019Quote: ... and 5 mM MgCl2 at pD 7.5 corresponding to a pH reading of 7.1) at 25°C as follows: spin column at 2000 rpm for 2 min in a tabletop centrifuge (Beckman Coulter Allegra R Tabletop Centrifuge), discard the flow-through ...
-
bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
-
bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
-
bioRxiv - Genomics 2020Quote: ... followed by two size-selection steps with SPRIselect (1:0.6 and 1:0.2 sample vol. to beads vol.; Beckman Coulter, CA, USA). The libraries’ size distribution was determined by Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Bioengineering 2021Quote: ... containing 10% FBS and 1% PS and 50 nL of FDA-approved compounds (final concentration: 1 μM) using a pin tool (Biomek FX, Beckman Coulter). Cells were added to each well (1,500 cells/25 μL ...
-
bioRxiv - Microbiology 2020Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter, #A63880). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
-
bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
-
bioRxiv - Genomics 2023Quote: ... and paramagnetic beads (1:1 beads/sample) using the same purification protocol as with AMPure XP beads (Beckman Coulter, no. B37419AB). The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen ...
-
bioRxiv - Immunology 2024Quote: ... The product of the reverse transcription reaction was purified with AMPure XP beads at a 1:1 bead to sample ratio (Beckman Coulter). Purified cDNA was split in half to amplify the heavy and light chains in separate reactions ...
-
bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
-
bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed twice with PBS/1% BSA and resuspended in 500 µl PBS/1% BSA for analysis on a CytoFLEX S flow cytometer (Beckman Coulter, Australia). FITC was excited using a 488 nm laser and emission read at 525 nm using a 525/40 band-pass filter with a gain setting of 300 ...
-
bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA samples were then cleaned up of short fragments with AMPure XP beads (1:1 ratio; Beckman Coulter, Brea, CA, USA) and checked for cDNA quality and concentration on a 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
-
bioRxiv - Genomics 2021Quote: ... We further purified the materials eluted from the columns by adding 150 ul (1:1) of AMPure beads RNAclean XP (Beckman coulter, A63987) and incubating for 5 min ...
-
bioRxiv - Microbiology 2022Quote: ... The barcoded samples were pooled and purified using AMPure XP beads at a 1:1 ratio of sample:beads (Beckman Coulter; Brea, CA). The final libraries (350 – 800 ng DNA ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR product was examined on gel electrophoresis and cleaned up by the AMpure XP beads (PCR product: bead 1:1 v:v, Beckman Coulter, Krefeld, Germany). The generated libraries were pooled with equal molar DNA ...
-
bioRxiv - Genetics 2019Quote: ... and GlyA PE (1:200, Beckman Coulter, IM2211U, KC16). Finally ...
-
bioRxiv - Genetics 2019Quote: ... CD41 PE-Cy5 (1:200, Beckman Coulter, 6607116, P2) and GlyA PE (1:200 ...
-
Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 mM DTT) in a TLA100.3 rotor (Beckman) at 116,000 xg for 5 h ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... erythrocytes were lysed (1 ml of Versalyse, Beckman Coulter) and the lymphocytes fixed (25 μl of Fixative Solution ...
-
bioRxiv - Immunology 2020Quote: ... APC-A750 anti-CD3 (Beckman Coulter, clone UCHT-1), APC anti-CD4 (Beckman Coulter ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA in centrifuge tubes (#344060, Beckman Coulter). The samples were spun in an ultracentrifuge (rotor ...
-
bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
-
bioRxiv - Immunology 2022Quote: ... ViaKrome 808 viability dye (1/50 dilution, Beckman Coulter) and CellBlox blocking buffer (1:30 dilution ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
-
bioRxiv - Genomics 2023Quote: ... an additional 1× Agencourt AMPure XP bead (Beckman Coulter) clean-up was performed and eluted in 0.1× filtered TE ...
-
bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
-
bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
-
bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
-
bioRxiv - Microbiology 2019Quote: ... Amplified cDNA was pooled from the triplicate reactions and purified using a 1:1 ratio of AMPure XP beads (Beckman Coulter, Brea, CA) and quantified by Qubit High Sensitivity dsDNA kit (Thermo Fisher) ...
-
bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
-
bioRxiv - Microbiology 2022Quote: Chlorophyll (a+b) was extracted from 1 ml of algal or algal-bacterial pellets with ethanol (1 ml) and measured spectrophotometrically (Beckman Coulter, DU 800) (Wintermans and De Mots ...
-
bioRxiv - Genomics 2020Quote: PCR products for each sample were pooled by targeted gene in equimolar ratios then each pool was 1:10 diluted and subsequently cleaned with 1 volume of AMPure XP (Beckman Coulter, Brea, California, USA) before quantification ...
-
bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
-
bioRxiv - Microbiology 2023Quote: DNA cleanup was carried out after each step to remove DNA < 150 bp using a 1:1 ratio of AMPure® paramagnetic beads (Beckman Coulter, Inc., USA). Short read paired-end data was assembled using the de novo assembly algorithm ...
-
bioRxiv - Neuroscience 2021Quote: ... 1 mM DTT) in the ultracentrifuge tube (Beckman Coulter, 343778) on ice ...
-
bioRxiv - Cell Biology 2019Quote: ... followed by peroxidase labeled streptavidin (1:500, IM0309 Beckman Coulter) (type I and X collagen ...
-
bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified using 1× AMPure XP beads (Beckman Coulter), and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega) ...
-
bioRxiv - Bioengineering 2021Quote: ... diluted 1:500 using a CytoFLEX flow cytometer (Beckman Coulter). After gating on cells expressing ACE2-GFP fusion protein in FL1 channel ...
-
bioRxiv - Microbiology 2022Quote: ... Lysates were then centrifuged for 1 h (25,000 rpm, Beckman Coulter Avanti J26-XP centrifuge ...
-
bioRxiv - Microbiology 2020Quote: ... We used 1× Agencourt AMPure XP beads (A63882, Beckman Coulter) for sample clean-up and concentration of pooled barcoded samples ...
-
bioRxiv - Immunology 2021Quote: ... γδTCR-PE-Cy7 (1:20; clone IMMU510; B10247) from Beckman Coulter;NKG2D-PE (1:20 ...