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Citations for Beckman :
601 - 650 of 776 citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were purified using a ratio of 1:0.85 sample to bead ratio using Agencourt AMPure XP SPRI beads (Beckman Coulter). The final products were quantified on a D5000 Tapestation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: ... 10 mM Tris-HCl) and centrifugated at 107,000 g for 1 hour at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS supplemented with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
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bioRxiv - Biophysics 2023Quote: SpeMreBs in buffer S polymerized at room temperature with a volume of 200 μL were polymerized for 1–6 h and centrifuged (100,000 rpm at 23 °C for 120 min) in a TLA-100 rotor (Beckman Coulter) (2) ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
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bioRxiv - Microbiology 2023Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA, 1:100 dilution). Flow cytometry measurements were performed using a FACSCalibur (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Cell Biology 2023Quote: ... tared and ultracentrifuged for 1 h at 110,000x g using a Beckman L7-65 ultracentrifuge with a SW41Ti rotor (#331362, Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Illumina 16S Metagenomic Sequencing Library Preparation Protocol with the following changes: 10 μL of Amplicon product was purified using a 1:0.8 ratio of XP beads (Beckman Coulter), with beads resuspended in a final volume of 10 μL of nuclease-free water ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
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bioRxiv - Biochemistry 2024Quote: ... and the solution was centrifuged for 45 min at 20,000g at 1 °C (Beckman Coulter J2-21, JA-20 rotor). The supernatant was then loaded onto a 5 ml HisTrap column (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of productively infected cells (p24+ ...
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bioRxiv - Synthetic Biology 2024Quote: ... then approximately 1/10th of the volume of each pool was purified with a 0.9:1 ratio of AMPure XP beads:PCR mix (Beckman Coulter, A63881). The complete volume of the AMPure-cleaned 1st round PCR products for each library was used as the templates for a “2nd round PCR,” which added the remainder of the TruSeq HT i5 and i7 Illumina adaptor sequences ...
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bioRxiv - Cell Biology 2024Quote: ... The discontinuous gradient was spun for 18 hours at 100,000xg and collected into twelve 1 mL fractions (SW40 Ti rotor, Beckman Coulter). SEVs are typically located in fractions 6 and 7 ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
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bioRxiv - Microbiology 2024Quote: ... cells were washed in PBS containing 1% BSA and resuspended in PBS containing 0.5% BSA before fluorescence quantification by CytoFlex S Benchtop Flow Cytometer (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution).
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bioRxiv - Immunology 2024Quote: ... 1 million peripheral immune cells were pipetted into the DuraClone immunophenotyping tube (DuraClone IP Phenotyping kit from Beckman Coulter, USA). Each tube contained anti-CD45-KO ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... Cells were then washed two times with PBS supplemented with BSA (1%) and data recorded in a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Genetics 2024Quote: ... An aliquot of each cell suspension was diluted 1:1,000 with seawater and cell count was performed using flow cytometry (CytoFLEX S, Beckman Coulter) set at medium flow (30 µl/min) ...
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bioRxiv - Biophysics 2024Quote: ... 100 μL of Origami-R1-R2 was placed on top of a quasi-linear glycerol gradient (15–45%, in 1× TE-Mg2+ buffer) in a 0.8 mL Open-Top Thinwall Ultra-Clear tube (Beckman Coulter) and spun in an SW 55 Ti rotor (Beckman Coulter ...
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bioRxiv - Developmental Biology 2024Quote: ... These cDNA samples were cleaned up by 0.6:1 AMPure XP beads and relocated into 384-well plates using FxP (Beckman Coulter), quantified by Quant-iT PicoGreen (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We performed daily 1:210 dilutions of populations in YPD 30°C and SC 30°C and daily 1:28 dilutions of populations in SC 37°C using a BiomekFXp robot (Beckman Coulter). Before dilution ...
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bioRxiv - Developmental Biology 2020Quote: ... Subsequently libraries were subject to size selection to exclude DNA fragments larger than 1 kb using SPRIselect magnetic beads (Beckman Coulter). Samples were sequenced on a Illumina HiSeq 4000 platform to obtain 50 bp paired-end reads ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR products were analysed by electrophoresis in a 1% (w/v) agarose gel and purified using magnetic AMPure XP beads (Beckman Coulter), using a ratio of beads:PCR product of 2:1 ...
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bioRxiv - Molecular Biology 2020Quote: ... and 1 mM TCEP added before use) and centrifuged for 24 h at 28,000 rpm in a 70Ti rotor (Beckman Coulter, Inc.). The pellets were suspended in washing buffer (20 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... stained cells were resuspended in 1% paraformaldehyde (PFA) and acquired using Stained cells were acquired on Cytoflex (Beckman Coulter, Brea, CA) and analysed with FlowJo v10.0.8 (Tree Star ...
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bioRxiv - Biochemistry 2020Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 g for 1 hour (Beckman Coulter JA- 20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... the cell suspensions were diluted in 1 mL and loaded onto a cytoflex-LX flow cytometer (Beckman Coulter, Brea, CA, USA) with a 375 nm laser ...
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bioRxiv - Molecular Biology 2020Quote: ... sucrose cushion prepared in PSB with 100 nM CaCl2 and spun for 1 hour at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA) to remove aggregates ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Cancer Biology 2020Quote: ... The emulsions were then broken using perfluoro-1-octanol and the aqueous fraction was diluted in water and collected for DNA purification with SPRI beads (Beckman Coulter). Sample indexes and Illumina adaptor sequences were then added via a 10 cycle PCR reaction and the amplified material was then SPRI purified a second time ...