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Citations for Beckman :
251 - 300 of 776 citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Dialysate was mixed with 1 ml of 70% Histodenz in iso-osmolar Rb150 + 1 mM MgCl2 in an ultraclear SW60 tube (Beckman-Coulter, Brea, CA), then overlaid with 1.6 ml 25% Histodenz in Rb150 + 1 mM MgCl2 and finally 800 μl Rb150 +1 mM MgCl2 ...
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bioRxiv - Immunology 2022Quote: ... Immunofluorescence-labelled cells were washed twice in PBS-EDTA after incubation with the antibody and then subjected to flow-cytometry analysis using a Gallios flow cytometer (Beckman Coulter). Cytotoxicity of the compounds was assessed using the MTT assay (Roche ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Microbiology 2020Quote: ... Whole blood staining was done with Alexa Fluor®647 labelled-VLP (2.5µg/ml) and cell subsets were discriminated using the following antibody panel: CD45 (Clone J33; Beckman Coulter, Brea, CA), CD11c (Clone BU15 ...
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bioRxiv - Developmental Biology 2022Quote: ... The samples were resuspended in 200 μl and stained on ice 30 minutes with APC conjugated anti-CD56 antibody (Beckman Coulter) and subsequently washed ...
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bioRxiv - Neuroscience 2021Quote: ... Labeling was done with anti-HLA-DR-PB and anti-CD16-FITC (Duraclone pre-coated tubes or separate antibodies, all from Beckman-Coulter) and anti-CD14-PEvio770 (Miltenyi ...
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bioRxiv - Microbiology 2020Quote: ... Duplicates of samples without EdU were analysed by FACS and stained in permeabilized samples with 0.05% Saponin using anti-Gag antibody conjugated to Rhodamine (Beckman Coulter, #6604667) diluted 1:500 in PBS-BSA 0.5% ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Cancer Biology 2024Quote: ... CD138 and CD70 for CAR-T (see table of antibodies) and tumor identification and analyzed by flow cytometry using a CytoFLEX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo 10.10.0 software ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Genomics 2020Quote: PCR products for each sample were pooled by targeted gene in equimolar ratios then each pool was 1:10 diluted and subsequently cleaned with 1 volume of AMPure XP (Beckman Coulter, Brea, California, USA) before quantification ...
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bioRxiv - Microbiology 2023Quote: DNA cleanup was carried out after each step to remove DNA < 150 bp using a 1:1 ratio of AMPure® paramagnetic beads (Beckman Coulter, Inc., USA). Short read paired-end data was assembled using the de novo assembly algorithm ...
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bioRxiv - Neuroscience 2021Quote: ... 1 mM DTT) in the ultracentrifuge tube (Beckman Coulter, 343778) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C for 1 hour (JA 25.5 rotor, Beckman Coulter). The supernatant was kept and clarified by a 0.45 µM syringe filter (Pall Corporation) ...
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bioRxiv - Molecular Biology 2021Quote: ... Amplicons were purified using 1× AMPure XP beads (Beckman Coulter), and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega) ...
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bioRxiv - Bioengineering 2021Quote: ... diluted 1:500 using a CytoFLEX flow cytometer (Beckman Coulter). After gating on cells expressing ACE2-GFP fusion protein in FL1 channel ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then centrifuged for 1 h (25,000 rpm, Beckman Coulter Avanti J26-XP centrifuge ...
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bioRxiv - Microbiology 2020Quote: ... We used 1× Agencourt AMPure XP beads (A63882, Beckman Coulter) for sample clean-up and concentration of pooled barcoded samples ...
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bioRxiv - Immunology 2021Quote: ... γδTCR-PE-Cy7 (1:20; clone IMMU510; B10247) from Beckman Coulter;NKG2D-PE (1:20 ...
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bioRxiv - Immunology 2020Quote: ... and RD1-conjugated anti-HIV-1 p24 (KC57, Beckman Coulter) to identify infected cells ...
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bioRxiv - Genomics 2021Quote: ... Amplicons were purified using 1× AMPure XP beads (Beckman Coulter), and cleaned-up PCR product concentrations were measured using a QuantiFluor® dsDNA System assay kit on a Quantus™ Fluorometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... with 1 drop of flow check beads (Beckman Coulter, USA) was sorted into each well of an Eppendorf 384-well plate (Cat no 34028 ...
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bioRxiv - Microbiology 2023Quote: ... and HIV-1 core antigen-FITC (KC57, Beckman Coulter; 6604665).
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bioRxiv - Genetics 2023Quote: ... and purified using 1× AMPure XP beads (Beckman Coulter A63881). The isolated ePCR products were inserted into a BsmBI-digested lentiV2-mU6-evopreQ1 vector via Gibson assembly (NEB E2621L) ...
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bioRxiv - Cancer Biology 2023Quote: ... with 1 drop of flow check beads (Beckman Coulter, USA) was sorted into each well of an Eppendorf 384-well plate (Cat no 34028 ...
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bioRxiv - Genomics 2023Quote: ... and purified using 1× AMPure XP beads (Beckman Coulter, A63881). Round one PCR amplicons were used in the 2nd round of PCR to add Illumina adapter and index sequences ...
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bioRxiv - Genomics 2023Quote: ... and purified using 1× AMPure XP beads (Beckman Coulter, A63881). The isolated ePCR products were then inserted into a BsmBI–digested lentiV2-mU6-evopreQ1 vector via Gibson assembly (NEB ...
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bioRxiv - Synthetic Biology 2023Quote: ... purified using 1× volume of AMPure XP (Beckman Coulter, A63881) and eluted with water ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was ultracentrifuged (Beckman 45Ti, 40,000 rpm, 1 h), and the supernatant was applied to an anti-FLAG M2 affinity gel (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... After ultracentrifugation (Beckman Ti70 rotor, 150,000 × g for 1 h), the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [TN buffer + 1% (w/v ...
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bioRxiv - Plant Biology 2024Quote: ... and 1 mL polypropylene tubes (Open-Top Thickwall, Beckman Coulter). The pellets (containing the membrane fractions and membrane-associated proteins ...
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bioRxiv - Microbiology 2024Quote: ... HIV-1 Core antigen-FITC (KC57) was obtained from Beckman Coulter ...
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bioRxiv - Bioengineering 2021Quote: The following antibodies were used for flow cytometry staining in a 96-well format: PE-labelled anti-human CD4 (Beckman Coulter, #A07752), APC-labelled anti-human CD8 (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA).
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bioRxiv - Bioengineering 2024Quote: ... the stained cells were washed twice to remove any unspecific binding antibodies and were then subjected to measurement using a Beckman Cytoflex S flow cytometer (Beckman, CA, USA). The resulting data were analyzed using FlowJo software (Tree Star ...
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bioRxiv - Immunology 2023Quote: ... frozen splenocytes were stained as described above using surface antibodies against following TCR Vβ segments (clone, brand, fluorophore): VB3 (CH92, Beckman Coulter, FITC), VB4 (WJF24 ...
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bioRxiv - Biochemistry 2021Quote: ... NaCl solution was added to 1 M and the solution was centrifuged for 10 min at 12,000 × g at 1°C and for 20 min at 38,000 x g at 1°C (Beckman Coulter Avanti J-25I, JA 25.50 rotor). CHAPS was added to 0.02% ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Cancer Biology 2020Quote: ... Tie2-PE and CD34-FITC antibodies (eBioscience 17-5821-81, 17-1171, 12-5987, 25-0411, 11-0341-82) by flow cytometry (Beckman Coulter CyAn ADP) or confocal microscopy using Confocal Microscope Zeiss LSM 880 with Airyscan.
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals for the antibody-receptor complex on the cell surface were then quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA). Data were analyzed using FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM GTP in a TLA110 rotor (Beckman, Palo Alto, CA) at 80,000 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C for 1 h with MLS-55 rotor (Beckman Coulter). The pellets were lysed in 40 μL of radioimmunoprecipitation (RIPA ...
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bioRxiv - Genomics 2021Quote: ... following ultra-centrifugation 30,000g for 1 h (Rotor:Beckman Coulter, MLS-50) at 4°C ...
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bioRxiv - Genomics 2021Quote: ... following ultra-centrifugation 30,000xg for 1 h (Rotor:Beckman Coulter, MLS-50) at 4°C ...
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bioRxiv - Immunology 2020Quote: ... anti-CD45RO ECD (clone UCHL1, Beckman Coulter, Cat. M2712U, 1:100), anti-PD1 APC (clone NAT105 ...
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bioRxiv - Molecular Biology 2023Quote: ... After ultracentrifugation (Beckman Ti70 rotor, 150,000 x g for 1 h), the collected membrane fraction was homogenized with a glass Dounce homogenizer in 20ml buffer [HN buffer + 1% (w/v ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... 1 mM EDTA) with a 50.2 Ti rotor (Beckman Coulter, CA) at 35,000 rpm for 90 min ...
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bioRxiv - Immunology 2023Quote: ... mouse anti-CD79b PE (clone CB3-1) (IM1612U) was from Beckman Coulter ...