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Citations for Beckman :
601 - 650 of 2165 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The PNS- Nycodenz gradient was centrifuged for 1 h at 141,000 x g at 4°C (46,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... amplified by PCR for 6 cycles and purified with AMPure XP beads (Beckman Coulter). The final libraries were pooled and sequenced using the onboard cluster method ...
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bioRxiv - Genomics 2020Quote: ... (6) the library fragments were purified with AMPure XP Beads (Beckman Coulter, Shanghai, China) to select the insert fragments of 150-200 bp in length ...
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bioRxiv - Biophysics 2022Quote: ... The 6 kb long 64x repeat was then purified using SPRIselect beads (Beckman Coulter) at a 1 to 0.4 v/v ratio ...
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bioRxiv - Genomics 2021Quote: ... products were assessed on 6% TBE-gel and purified using 1.0X AMPure (Beckman Coulter) and added to the second PCR reaction that appended dual sample indexes and flow cell adapters ...
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bioRxiv - Genomics 2021Quote: ... products were assessed on 6% TBE-gel and purified using 0.6X AMPure (Beckman Coulter) and added to the second PCR reaction that appended PacBio sample indexes ...
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bioRxiv - Bioengineering 2021Quote: ... products were assessed on 6% TBE-gel and purified using 1.0X AMPure (Beckman Coulter) and added to the second PCR reaction that appended dual sample indexes and flow cell adapters ...
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bioRxiv - Plant Biology 2024Quote: ... The second PCR contained 6 µL of purified PCR product (using SPRIselect beads; Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2020Quote: ... which were centrifuged at 50,000rpm for 4 hours at 4°C using a TLS55 rotor and an Optima MAX-XP Ultracentrifuge (Beckman Coulter). The sample was physically fractionated into 10 fractions of 85 uL each ...
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bioRxiv - Molecular Biology 2022Quote: ... 800 µl of EDTA-treated lysate was layered on top of the sucrose gradient and ultracentrifuged at 35000 rpm for 4 hours at 4 °C in a SW41Ti rotor (Beckman). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... all CCMV CP preparations were purified two times using an ultracentrifuge and 30% sucrose cushion – first with additional 0.5% Triton X-100 and the second time without Triton X-100 (4 h, 50 000 rmp, 4°C; Type 70Ti rotor, Beckman) and the pellet was then dissolved in buffer A ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Neuroscience 2023Quote: ... the samples were filtered with a membrane pore size of 0.2 μm and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C in a TLA 100.3 rotor (Beckman). P13 ...
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bioRxiv - Cell Biology 2022Quote: ... 4°C (Type 45 Ti rotor, Beckman Coulter) for 30 min to collect supernatant ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C (Beckman Coulter Optima LE-80K Ultracentrifuge). The clarified lysate was placed in a beaker on a stir plate at 4 °C and concentrated ammonium sulfate (5 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... for 4 h with SW28 rotor (Beckman Coulter). The concentrated vector was stored at −80 °C until use ...
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bioRxiv - Biochemistry 2022Quote: ... 4 °C on a Sorvall WX90 ultracentrifuge (Beckman) with SW55Ti rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... 4°C in SW-41Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Plant Biology 2021Quote: ... 4 °C for 18 h (Beckman SW41 rotor). The sugar solution contains 0.65 M sucrose ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C using a SW41Ti rotor (Beckman Coulter). A BioComp fractionator was used to collect fractions 1 – 16 ...
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bioRxiv - Neuroscience 2022Quote: ... at 4°C in a Beckman ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Systems Biology 2022Quote: A particle size analyzer (Multisizer 4, Beckman Coulter) was used to count the number of cells ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C (Beckman Coulter, JA 25-50 rotor) for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Beckman Coulter, JA 25-50 rotor) for 10 min and the cell lysates were incubated with 1 mL of prewashed Ni-NTA Agarose beads (QIAGEN ...
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bioRxiv - Molecular Biology 2021Quote: ... triglycerides and non-esterified fatty acids using a serum analyzer (AU480 Chemistry analyzer Beckman Coulter). Plasma levels of leptin and insulin were assessed using ELISAs used according to the manufacturer’s instructions (R&D Systems ...
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bioRxiv - Immunology 2020Quote: ... nucleic acids were extracted from sorted single cell using RNAClean XP SPRI beads (Beckman Coulter), and RNA was hybridized first using RT primer (/5BiosG/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT TVN ...
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bioRxiv - Developmental Biology 2020Quote: ... a CytoFLEX 3 laser 13 colour flow cytometer (Beckman Coulter) was used ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Microbiology 2024Quote: ... The 1 ml fraction containing the phage was then placed on a 40% (w/v) - 60% (w/v) sucrose gradient for 1 hour at 63,400 x g at 4°C (Beckman Coulter SW 40 Ti). The 1 ml fraction was then placed in a 50 kDa dialysis membrane (Spectrum Labs ...
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bioRxiv - Biochemistry 2024Quote: ... The A2aR-expressing membrane fraction was obtained by ultra- centrifugation at 100,000 g for 1 hour at 4°C in a Type 70 Ti Fixed-angle rotor (Optima XPN ultracentrifuge, Beckman Coulter Life Sciences). The pellet (membrane fraction ...
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bioRxiv - Immunology 2021Quote: ... were incubated with monoclonal antibodies at 4°C for 30 min and then fixed with 4% paraformaldehyde before analysis on a Cytoflex cytometer (Beckman Coulter). Data were analyzed by Flow Jo software v.10.7 (BD Biosciences) ...
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bioRxiv - Cell Biology 2021Quote: ... and loaded onto of a step-wise sucrose gradient as described by the manufacturer and subjected to ultracentrifugation at 150,000 xg for 4 h at 4 °C using SW50.1 rotor (Beckman Coulter, Mississauga, ON). Fractions were then collected and subject to denaturation with Laemmli buffer until further use.
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bioRxiv - Molecular Biology 2024Quote: ... They were cherrypicked from the original library plates and 4 crRNAs per target were pooled (4 x10 μl) and re-arrayed using a Biomek-FX automated pipetting system (Beckman Coulter). Edit-R CRISPR-Cas9 Synthetic tracrRNA (Horizon Discovery Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... 21000 rpm for 5 h (Beckman SW41Ti). The band of the expected density was pooled using a syringe and the buffer was exchanged to the E-buffer using 100kDa VivaspinTM columns (Cytiva) ...
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bioRxiv - Cell Biology 2022Quote: ... Equal volumes were distributed into 6 ultra-clear thin wall tubes (Beckman Coulter Cat. 344058) and ultra-centrifuged at 50,000 G for 2 hours at 160C in a Beckman Coulter Optima XE ultracentrifuge to precipitate lentiviral particles ...
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bioRxiv - Cell Biology 2024Quote: ... 6-8 PCR reactions were pooled and purified with AMPure XP SPRI Reagent (Beckman Coulter), first at 0.6x and then at a 1x volumetric ratio ...
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bioRxiv - Systems Biology 2020Quote: ... and the resultant supernatant was ultracentrifuged at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2; Beckman Coulter 343778 1 ml uncapped tubes).
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bioRxiv - Physiology 2021Quote: ... sEV were further isolated from lEV-depleted supernatants following a 100,000 xg ultracentrifugation step for 1 hour at 4°C (rotor MLA-50, Beckman Coulter Optima MAX-XP Ultracentrifuge) and two washes in NaCl before resuspension in NaCl.
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was filtered by 0.22 μm nylon membrane followed by centrifugation through a cushion of 50 % (w/v) sucrose with 36,000 rpm at 4 °C for 4 h (Beckman SW41 Ti rotor). The pellet was resuspended with PBS and the copy number of DWV was quantified by qRT-PCR.
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bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
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bioRxiv - Microbiology 2020Quote: ... 4°C in SW 60 Ti rotor (Beckman Coulter) to pellet cyanophage particles ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW32Ti rotor, Optima L-80XP ultracentrifuge, Beckman) with the maximum acceleration and minimum deceleration ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C in swinging-bucket rotor SW40 (Beckman Coulter). Fractions (1 ml ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C cells were resuspended in Isoflow (Beckman) for further reading the percentage of fluorescent labelled cells on the cytometer (FC500 ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C (SW41 Ti Swinging-Bucket rotor, Beckman & Coulter). Bands containing virus particles were extracted with a syringe ...