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Citations for Beckman :
551 - 600 of 2165 citations for 6 Methyl 5 4 phenyl 1 3 thiazol 2 yl 2 trifluoromethyl nicotinic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
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bioRxiv - Immunology 2020Quote: ... KIR2DL2/3/S2 (GL183, PE/Cy5.5, Beckman Coulter,), NKG2C (134591 ...
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bioRxiv - Systems Biology 2023Quote: ... enumerated using a Multisizer 3 Coulter Counter (Beckman,), and then seeded at the desired density in 6-well tissue culture plates (TPP ...
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bioRxiv - Genetics 2022Quote: ... 5 mL (Beckman Coulter, B23317) using a DynaMag™-2 Magnet magnetic stand (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... the CP was centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TLA-100.3 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TL-100.1 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge to pellet the reassembled VLPs ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Microbiology 2020Quote: ... 4°C in an SW55Ti rotor (Beckman), and the virus pellet harvested for analysis by WB with αGag.
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Cell Biology 2020Quote: ... 4 °C with 70Ti rotor (Beckman Coulter) to collect sEV pellets in double 0.22 μm-filtered PBS ...
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bioRxiv - Microbiology 2024Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Polysomes profiles were obtained using Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Microbiology 2024Quote: ... 4°C (Optima TLX Ultracentrifuge, Beckman Coulter). The pellet was resuspended in 200 µL of ultrapure water.
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bioRxiv - Microbiology 2022Quote: ... 4 °C in SW41 rotor (Beckman Coulter). Samples were fractionated and collected using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C in SW28 rotor (Beckman Coulter). Gradients were fractionated using Biocomp Piston Gradient Fractionator (BioComp Instruments) ...
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bioRxiv - Developmental Biology 2024Quote: ... at 4°C (JLA 8.1000 rotor, Beckman), lysed in Lysis buffer (20 mM Hepes ...
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bioRxiv - Biophysics 2024Quote: ... 4 °C (Type 70 Ti, Beckman Coulter), the supernatant was purified by IMAC (5 mL HiTrap Chelating HP ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Cell Biology 2021Quote: ... then finally at 100,000 g for 4 h at 4 ℃ using an SW28 rotor (Beckman Coulter, Fullerton, CA, USA). The pelleted exosomes were collected then subsequently resuspended in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Hettich) at 4°C and then centrifuged at 40000 rpm for 4h30 at 4°C using SW41Ti rotor (Beckman). The supernatant was then UV inactivated at a wavelength of 254 nm for 4 min (3 times ...
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bioRxiv - Microbiology 2023Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k x g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Genetics 2024Quote: ... and spun at 70,000 rpm for 4 hours at 4°C using a TLA 100.3 Rotor in an Optima Ultracentrifuge (Beckman, 361889). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... and the remainder was then loaded onto a 20%-60% sucrose cushion and centrifuged at 25,000 rpm at 4°C for another 4 hours using the SW41 rotor (Beckman). The initial 5 μL sample and each sucrose gradient layer were then absorbed ...
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bioRxiv - Microbiology 2024Quote: ... The lysates containing matured PsVs were loaded on an OptiPrep gradient that had been stabilized at least 1 h and centrifuged at 50k ×g for 3.5-4 h at 4°C in a SW-55Ti rotor (Beckman). Fractions were collected in siliconized tubes and subjected to SDS-PAGE followed by Coomassie blue staining to assess the abundance of L1 and L2 proteins ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Systems Biology 2020Quote: ... Next the cultures were diluted 1:40 with 0.5M NaCl and immediately measured in Multisizer™4 COULTER COUNTER® (Beckman Coulter) (Coulter ...
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bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
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bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
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bioRxiv - Molecular Biology 2023Quote: ... The cell lysate was centrifuged at 4 °C at 35000 rpm for 1 hour with Beckman Optima™ L-80XP Ultracentrifuge equipped with Ti45 rotor (Beckman, USA). The pellet containing membranes and cell debris was discarded ...
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bioRxiv - Cell Biology 2023Quote: ... the supernatants were transferred into SW60 tubes (in vivo studies 1–4) or SW40 tubes (donated patients) (Beckman Coulter Inc., Brea, USA) and centrifuged at 10,000xg for 35 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... The PNS- Nycodenz gradient was centrifuged for 1 h at 141,000 x g at 4°C (46,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The top agar was then scraped off the LB agar and centrifuged for 26,000 x g at 4°C for 1 hour (Beckman Coulter JA-17). The supernatant was then removed ...
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bioRxiv - Microbiology 2024Quote: ... Crude ribosomes were separated from other low molecular weight cellular contents by centrifugation at 35000 rpm (126,000g) for 1 hour at 4 °C (Beckman rotor Type 70Ti). The crude ribosome pellet was resuspended in 10 mL HMA-10 buffer and layered on top of a 10 mL 32% sucrose solution in HMA-10 buffer and centrifuged for 16 hours at 37,000 rpm (140,000g ...