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Citations for Beckman :
551 - 600 of 1463 citations for 2 Chloro 3 4 cyanobenzoyl pyridine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were then layered on 100 μL of 32% sucrose cushion and centrifuged at 42,800 rpm for 3 hours in a Beckman TLA 100 rotor using the Optima-Max TL ultracentrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... HUVECs or LECs were infected for 3 days and the GFP positive population was quantified by CytoFLEX S (Beckman Coulter). Here ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Cancer Biology 2021Quote: ... decrosslinked material was purified with 2 x AMPure XP beads (Beckman Coulter, Cat #A63881) and eluted in 30 μL of 10 mM Tris-Cl ...
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bioRxiv - Neuroscience 2020Quote: ... DRG were then centrifuged for 2 min at 1000 rpm (Beckman Allegra X15R, 233xg), supernatant aspirated ...
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bioRxiv - Molecular Biology 2020Quote: ... and centrifuged for 2 hours at 55,000 rpm (TLS-55 rotor, Beckman Coulter, USA). 11x 200 μl fractions were harvested and subjected to FLAG-IP ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 300μl PBS/2%FBS and FACS analysed on Gallios® (Beckman Coulter) or ARIAIII (BD) ...
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bioRxiv - Biophysics 2023Quote: ... 2 mM DTT) with 0.7 M sucrose in Type 45 Ti tubes (Beckman-Coulter). Ribosomes were pelleted by centrifugation at 27,000 rpm (57,000 xg ...
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bioRxiv - Cancer Biology 2023Quote: ... concentrated for 2 h by ultracentrifugation in swing-out rotor SW-32Ti (Beckman Coulter), 26,000g ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Illumina RNASeq sequencing (HiSeq 2000, 2 × 75 pb) and trimming were performed by Beckman Coulter Genomics ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultra-centrifuged for 2 hours at 20,000 rpm in an SW28 rotor (Beckman). Viral pellets were resuspended in 250 µL PBS and flash-frozen ...
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bioRxiv - Systems Biology 2023Quote: ... Adapter ligation was followed by 2 successive Agencourt AMPure XP bead purifications (Beckman Coulter) and library amplification by a Plasmodium-optimized KAPA PCR amplication protocol ...
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bioRxiv - Microbiology 2024Quote: ... and 2 mM dithiothreitol (DTT)) and centrifuged in an SW-41 Ti rotor (Beckman) for 2 h at 40,000 rpm ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR-2 product was cleaned up with Ampure XP bead-based reagent (Beckman Coulter). Library concentration and purity was verified using the Qubit dsDNA HS kit (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... DNA was cleaned up and concentrated with 2× AMPure XP beads (Beckman Coulter; A63882). DNA was eluted in 30µL of Buffer EB (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration of the cleared lysate was measured by nanodrop and 500 μg of this RNA was loaded onto the gradient and run for 2.5 hr at 220,000 g and 4°C in an SW41-rotor (Beckman). Gradients were then run at 850 μL/min in a density gradient fractionation system (Teledyne Isco) ...
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bioRxiv - Microbiology 2022Quote: ... for 3h at 4°C in a Beckman ultracentrifuge with the SW40ti rotor head (Beckman, Brea, California, USA) using open top polyallomer tubes (Seton Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting solution was centrifuged at 164,000 × g (30,000 rpm) for 4 h at 20°C (Optima XPN-80 Ultracentrifuge, Beckman) to remove any remaining nanotube aggregates.
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bioRxiv - Bioengineering 2020Quote: ... larger vesicles were depleted by centrifugation for 30 minutes at 10,000xg at 4°C (SW28 rotor, Beckman Coulter). In the final step ...
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bioRxiv - Biochemistry 2020Quote: ... 300mM NaCl (pH 7.0) was performed at 4 ⁰C on an Optima XL-A Analytical Ultracentrifuge (Beckman Coulter). Equilibrium radial distributions at 10,000 and 15,000 RPM of Sfh5 at concentrations of 10 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was transferred into new centrifuge tubes and centrifuged at 40,000g (P40) or 100,000g (P100) for one hour (4°C, TLA100.3, Optima TLX Ultracentrifuge; Beckman Coulter) to pellet EVs and other particles as noted in figure legends ...
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bioRxiv - Biochemistry 2020Quote: ... Beckman CoulterTM at 150.000 g for 120 minutes at 4°C with a TLA-55 Rotor (Beckman CoulterTM) to pellet EVs ...
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bioRxiv - Microbiology 2020Quote: ... Then 4 ml of the adjusted lipoprotein was layered under 8 ml of 0.9% NaCl and centrifuged at 49,000 rpm for 4 hours at 10°C (NVTi 65 rotor; Beckman). The HDL (lower band ...
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bioRxiv - Biochemistry 2021Quote: ... and separated by ultracentrifugation in a TLA120.2 rotor (Beckman Coulter; 1.5 h at 278,000 x g, 4°C).
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was removed by centrifugation at 18k rpm for 60 min at 4 °C (Beckman JA-20). The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... and then sonicated and ultracentrifuged (440,000 g, 35 min, 4°C; MLA80 rotor, Beckman Optima™ MAX-TL). The supernatant was applied to a Ni-NTA column (Machery-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... pellets obtained by ultracentrifugation (100,000 x g in Ti45 Beckman Coulter rotor for 70 min. at 4 ° C) were washed and resuspended in 1.25 ml buffer [0.25 M sucrose ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The samples were then centrifuged at 107,163.6 X g for 2.5 hours at 4°C in an ultracentrifuge (XPN 100, Beckman Coulter). After centrifugation ...