Labshake search
Citations for Beckman :
451 - 500 of 1491 citations for PCR Tube since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Cell Biology 2022Quote: ... 10 mM HEPES pH 7.4 and EDTA free protease inhibitor were prepared in polyallomer optiSeal ultracentrifuge tubes (11.2 ml capacity; Beckman Coulter) gradients were allowed to diffuse 1h at 20 °C ...
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bioRxiv - Microbiology 2019Quote: ... The entire 3 ml mixture was placed at the bottom of a Beckman SW41 ultracentrifuge tube (Beckman, Munich, Germany) and subsequently overlaid with 5 ml of ice-cold 30% Optiprep in TNE and 4 ml of ice-cold 5% Optiprep in TNE ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μL of 1% FBS/DPBS and transferred to polystyrene tubes for flow cytometric analysis (Beckman Coulter HyperCyAn ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Cell Biology 2020Quote: ... and 5 % solutions on top of each other in a 13.2 ml open-top polyallomer tube (331372, Beckman Coulter). The 1 ml concentrates were overlaid on top of the gradient ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were then centrifuged for 40 min at 350,000 g in 500 μL polycarbonate centrifuge tubes (Beckman Coulter) at 4°C in an Optima MAX-XP Ultracentrifuge with a TLA 120.1 rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... buoyant density of 1.71 g ml−1) in polyallomer tubes before sealing and ultracentrifugation at 152,000 × g (50,000 rpm) in a MLN80 rotor (Beckman-Coulter) for 72 h at 25°C ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Genomics 2019Quote: ... The supernatant was then transferred to a clean 1.5 mL Eppendorf tube for a 2.2x Agencourt RNAClean XP beads clean-up (Beckman Coulter). Briefly ...
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bioRxiv - Cell Biology 2019Quote: ... The supernatant was then transferred to a fresh tube and centrifuged at high speed in a TLA-100.4 centrifuge (Beckman) at 70000 rpm for 30 min ...
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bioRxiv - Physiology 2019Quote: ... samples were thawed on ice and transferred to 0.2 mL polycarbonate thick-wall tubes (Beckman Coulter, Danvers, MA, USA) and spun at 100,000xg for 20m at 4°C in Optima Max ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: Samples of filtered 0.5 mL plasma or CSF were added to 3.5 mL Open-Top Thickwall Polycarbonate ultracentrifuge tubes (Beckman Coulter), and PBS was added to fill tubes to the top ...
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bioRxiv - Microbiology 2020Quote: ... density gradient centrifugation was performed in 5,1 ml quick-seal tubes in a NVT 65.2 rotor (near vertical) using Optima L-90K centrifuge (Beckman coulter). CsCl medium having an average density of 1,72g/ml was loaded with 6μg of total extracted DNA ...
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bioRxiv - Immunology 2021Quote: ... overlaid onto 5 ml of 10% sucrose in NTE (135 mM NaCl, 10mM TrisCl, ph 7.50, 1mM EDTA) in ultra-centrifuge tubes (Beckman) and centrifuged at 25,000 rpm for 90 minutes at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... and the tube was centrifuged for 2 hours at 190,000 x g in a swing-out rotor (SW40 Ti; Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: ... A 0.6-1.9 M continuous sucrose gradient was prepared in 10 mM HEPES pH 7.2 in ultracentrifuge tubes (Beckman Coulter) that were coated with Sigmacote (Sigma #SL2) ...
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bioRxiv - Cell Biology 2022Quote: ... Resuspended pellets were then subjected to an iodixanol density gradient in 5-mL Ultra-Clear centrifuge tubes (Beckman Coulter). The tubes were first filled with a two-layer gradient ...
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bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
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bioRxiv - Plant Biology 2022Quote: ... was gently layered on top of 4.4 mL sucrose gradient solution (15 to 55%) in a 5.1 mL Quick-Seal polypropylene tube (Beckman Coulter) and centrifuged at 45,000 rpm for 65 min at 4°C (Sorval NVT 90 ...
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bioRxiv - Molecular Biology 2023Quote: ... 72 μL of 10% sarkosyl was added to 650 μL of each sample in ultra-centrifuge tubes (Beckman Coulter) and samples were rotated end-over-end at room temperature for one hour ...
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bioRxiv - Neuroscience 2022Quote: ... myelin was layered onto 3 mL ice cold 0.85 M sucrose solution in a 10 mL ultracentrifuge tube (Beckman) before centrifugation at 75 000 x g for 30 min at 4 °C on a Beckman Ultracentrifuge with MLA-55 rotor (acceleration 7 ...
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bioRxiv - Cell Biology 2022Quote: ... and 3.5 mL of 5% iodixanol in a 16.8 mL open top polyallomer tube (Beckman Coulter, Fullerton, California, USA). One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Linear 10–30% glycerol gradients were prepared in 11□mm□×□34□mm polyallomer centrifuge tubes (Beckman Coulter, Brea, CA) with HEM buffer [25 mM HEPES (pH 7.5) ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg/mL Bacitracin) underlay was gently pipetted under the supernatant in polyallomer ultracentrifuge tubes (Beckman-Coulter, Cat #: 326823). The virus was ultracentrifuge concentrated using a SW32 Ti swinging bucket rotor 24,000 rpm for 2 h at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Biochemistry 2022Quote: ... The tubes were then centrifuged at 150,000xg for 16 h in an SW41 Ti swinging-bucket rotor (Beckman Coulter). After centrifugation ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... Layers of 2.1 mL of each solution were successively poured into 12.5 mL polyallomer tubes (Beckman-Coulter, Cat. # 331372), starting from the most concentrated solution (50 % ...
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bioRxiv - Pathology 2023Quote: ... and culture supernatant was concentrated by ultracentrifugation (2h at 26,000rpm; Ultraclear Tubes; SW28 rotor and Optima L-100 XP Ultracentrifuge; Beckman). Viruses were suspended in cold sterile PBS and titrated by transduction of Jurkat cells for 48h ...
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bioRxiv - Neuroscience 2023Quote: ... and loaded onto a two-step CsCl step gradient 41 in a thick wall ultracentrifuge tube (Beckman Cat# 360743) Samples were loaded onto an ultracentrifuge (Sw32Ti rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... and 1.3 M sucrose and 0.5 mL of 1.8 M sucrose at the bottom) in ultracentrifuge tubes (14 × 89 mm, Beckman Coulter). The sucrose gradients contained 0.03% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... The tubes were centrifuged at 100,000 × g for 2 hours using a SW 55Ti swinging-bucket rotor (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... Then 90 ul of the mix was transferred to a fresh tube and cleaned with SPRIselect beads (Beckman Coulter) using a two-sided (1.2x-0.5X ...
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bioRxiv - Cell Biology 2023Quote: ... and the full tube was transferred to a pre-cooled metal holder of a SW32_Ti ultracentrifuge rotor (Beckman-Coulter). Tubes were spun for 30 min at 103.000 rcf at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Then AAVs were purified by ultracentrifugation (OptimaXE-90, Type 70 Ti rotor with 32.4mL OptiSealTM tubes, Beckman Coulter, USA). with 58400 rpm for 145 minutes in 18°C with Iodixanol density gradient solutions (15% ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei were passed through a 40um diameter strainer and the lysate was transferred into a 15ml ultracentrifuge tube (Beckman). 9ml of ice cold sucrose solution (30.78g Sucrose ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 5.1 ml of the solution was transferred to an ultracentrifuge tube (Beckman Coulter QuickSeal, 13 mm × 51 mm) and heat-sealed ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cell Biology 2024Quote: ... 1.6 ml of each fraction was very slowly layered in a 14 ml ultracentrifuge tube (Beckman Coulter, California, USA), starting from higher to lower concentrations ...
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bioRxiv - Cancer Biology 2020Quote: ... The PCR product was purified by Agencourt Ampure XP PCR Purification beads per the manufacturer’s protocol (Beckman Coulter, Brea, CA). One microgram of Cas9 plasmid and 0.3 μg of each gRNA gBlock (pair ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Microbiology 2019Quote: ... including library amplification for 15 PCR cycles using custom indexed primers and post-PCR clean-up with 0.85x volume Ampure XP (Beckman Coulter). Libraries were quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen ...
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bioRxiv - Pathology 2023Quote: ... Ligated cDNAs were amplified following 15 PCR cycles and PCR products were purified using AMPure XP Beads (Beckman Coulter Genomics). Libraries were validated using a Bioanalyzer on a DNA1000 chip (Agilent ...
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bioRxiv - Genetics 2023Quote: ... PCR products were purified with the Qiagen MinElute PCR Purification Kit and AMPure XP beads (Beckman Coulter, Cat. No. A63880) and resuspended in ultrapure nuclease-free distilled water ...