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Citations for Beckman :
451 - 500 of 1231 citations for 7 Hydroxy 4 methylcoumarin 3 acetic acid succinimidyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Samples were size selected using a 0.5X Ampure XP cleanup to remove large fragments followed by 3 consecutive 1.5X Ampure XP (Beckman Coulter # A63880) bead cleanup followed by a final 1.2X Ampure XP cleanup to completely remove all residual primers and resuspended in a final volume of 20 µL EB using the manufacturer’s recommended protocol.
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bioRxiv - Cell Biology 2019Quote: ... Monosomes were isolated by sucrose gradients (10-50%) and centrifuged at 40,000 rpm for 3 hours in a SW41 rotor (Beckman Coulter). The extracted RNA was size-selected from 15% denaturing PAGE gels ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...
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bioRxiv - Molecular Biology 2019Quote: ... Rnase-free sucrose) and gradients were centrifuged at 35,000 rpm for 3 hours 20 minutes using a SW41Ti rotor (Beckman Coulter). Absorbance was followed at 254 nm ...
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Immunology 2022Quote: ... The gradients were spun at 150,200 x g for 3 h in a SW41 Ti swinging-bucket rotor (Beckman Coulter). 13 fractions of 0.95 ml were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2022Quote: ... HUVECs or LECs were infected for 3 days and the GFP positive population was quantified by CytoFLEX S (Beckman Coulter). Here ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were then layered on 100 μL of 32% sucrose cushion and centrifuged at 42,800 rpm for 3 hours in a Beckman TLA 100 rotor using the Optima-Max TL ultracentrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Cell Biology 2020Quote: ... and then centrifuged (300 g for 5 min. at 4 °C) (Allegra X-30R Centrifuge, Beckman Coulter, USA) to obtain the myofiber-associated cell pellet ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration of the cleared lysate was measured by nanodrop and 500 μg of this RNA was loaded onto the gradient and run for 2.5 hr at 220,000 g and 4°C in an SW41-rotor (Beckman). Gradients were then run at 850 μL/min in a density gradient fractionation system (Teledyne Isco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Microbiology 2022Quote: ... for 3h at 4°C in a Beckman ultracentrifuge with the SW40ti rotor head (Beckman, Brea, California, USA) using open top polyallomer tubes (Seton Scientific ...
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bioRxiv - Physiology 2019Quote: ... Lysates (≈11 ml) were centrifuged at 26,000xg for 30 min at 4°C in a SW41Ti rotor (Beckman). The floating lipid droplet layers were harvested with a glass pipette and adjusted to 25% sucrose and 100 mM sodium carbonate ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting solution was centrifuged at 164,000 × g (30,000 rpm) for 4 h at 20°C (Optima XPN-80 Ultracentrifuge, Beckman) to remove any remaining nanotube aggregates.
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bioRxiv - Bioengineering 2020Quote: ... larger vesicles were depleted by centrifugation for 30 minutes at 10,000xg at 4°C (SW28 rotor, Beckman Coulter). In the final step ...
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bioRxiv - Biochemistry 2020Quote: ... 300mM NaCl (pH 7.0) was performed at 4 ⁰C on an Optima XL-A Analytical Ultracentrifuge (Beckman Coulter). Equilibrium radial distributions at 10,000 and 15,000 RPM of Sfh5 at concentrations of 10 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was transferred into new centrifuge tubes and centrifuged at 40,000g (P40) or 100,000g (P100) for one hour (4°C, TLA100.3, Optima TLX Ultracentrifuge; Beckman Coulter) to pellet EVs and other particles as noted in figure legends ...
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bioRxiv - Biochemistry 2020Quote: ... Beckman CoulterTM at 150.000 g for 120 minutes at 4°C with a TLA-55 Rotor (Beckman CoulterTM) to pellet EVs ...
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bioRxiv - Microbiology 2020Quote: ... Then 4 ml of the adjusted lipoprotein was layered under 8 ml of 0.9% NaCl and centrifuged at 49,000 rpm for 4 hours at 10°C (NVTi 65 rotor; Beckman). The HDL (lower band ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
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bioRxiv - Biochemistry 2021Quote: ... and separated by ultracentrifugation in a TLA120.2 rotor (Beckman Coulter; 1.5 h at 278,000 x g, 4°C).
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was removed by centrifugation at 18k rpm for 60 min at 4 °C (Beckman JA-20). The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... and then sonicated and ultracentrifuged (440,000 g, 35 min, 4°C; MLA80 rotor, Beckman Optima™ MAX-TL). The supernatant was applied to a Ni-NTA column (Machery-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... pellets obtained by ultracentrifugation (100,000 x g in Ti45 Beckman Coulter rotor for 70 min. at 4 ° C) were washed and resuspended in 1.25 ml buffer [0.25 M sucrose ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The samples were then centrifuged at 107,163.6 X g for 2.5 hours at 4°C in an ultracentrifuge (XPN 100, Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Molecular Biology 2019Quote: ... and centrifuged for 6 hours at 55,000 rpm at 4°C on a TLS-55 rotor (Beckman Coulter) in a TL-100 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2019Quote: ... After centrifugation at 20,000g for 30 min at 4°C on a SW 41 Ti rotor (Beckman Coulter), the mitochondrial interphase was transferred to a fresh tube and an equal amount of 1× TE buffer was added ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were ultracentrifuged at 95,000 rpm at 4°C for 1 h and 40 min using a TLA100.2 rotor (Beckman). Supernatants were saved if needed for further analysis ...
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bioRxiv - Plant Biology 2020Quote: ... and 100 μg/ml cycloheximide) and centrifuged for 1.5 h at 50,000 rpm at 4 °C in a SW55 Ti rotor (Beckman). RNA labeling was performed according to Zoschke et al ...
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bioRxiv - Zoology 2021Quote: ... then held at 4°C) at manufacturer-recommended enzyme concentrations and used AMPure XP beads (Beckman Coulter, #A63881) at a 1.5X ratio to retain only DNA from the digestion ...
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bioRxiv - Genetics 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% Bovine Serum Albumin (Tocris Bioscience ...