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Citations for Diagenode :
401 - 450 of 455 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Plant Biology 2019Quote: ... Loose chromatin was removed with 1% SDS prior to sonication on a Bioruptor™ (Diagenode). The immunoprecipitation step involved incubation with different antibodies (Flag M2 beads (sigma ...
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bioRxiv - Genomics 2023Quote: ... Crosslinked chromatin resuspended in 1 ml LB3 was then sonicated using a BioRuptor Pico (Diagenode) for 30 cycles of 30 seconds on/off ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: A total of 100 ng of total genomic liver DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A total of 100ng of total DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were centrifuged at 500g before proceeding with the nuclei isolation and transposition reaction using the ATAC-seq Kit from Diagenode (Diagenode Cat #C01080002). According to the results of the nuclei isolation optimization ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...
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bioRxiv - Developmental Biology 2021Quote: ... Immunoprecipitation was done using 15-20 μg of DNA and 1 μg of CTCF Ab (Diagenode:C15410210) and all steps were performed following the manufacturer instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... 1% SDS and sonicated to obtain approximately 200–500 bp chromatin fragments using a Bioruptor (Diagenode). Chromatin fragments were precleared with salmon sperm DNA (Trevigen ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM MgCl2,) and sonicated 10x with an interval of 30’’ on “medium” setting (Bioruptor300; Diagenode). Protein concentrations were measured using Quick Start™ Bradford 1x Dye Reagent from BIO RAD according to the manufacturers manual and equal amounts loaded for each lane.
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% SDS was then added followed by 3x 30sec sonication steps with a bioruptor sonicator (Diagenode) to solubilize the chromatin ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Microbiology 2023Quote: ... 1 ug of genomic DNA was sheared by sonication using a Picorupter (Diagenode s.a., Liege, Belgium) to obtain fragments with an average size of 300 bp ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Cell Biology 2023Quote: ... mRNA was adjusted to about 10Lng μl−1 in 100Lμl and fragmented using a Bioruptor ultrasonicator (Diagenode) with 30Ls on/off for 30 cycles ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was resuspended in 400µL of lysis buffer (1% SDS, 50mM Tris-HCl pH 7.5) and sonicated with the Pico Bioruptor (Diagenode) for 20 cycles (30sec ON/ 30sec OFF ...
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bioRxiv - Genomics 2023Quote: ... and 1 µg of DNA was purified and then sheared at 13 kb using the Megaruptor1 system (Diagenode). The library was size-selected ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Biochemistry 2024Quote: ... 1 x Halt Protease Inhibitor Cocktail) and water-bath sonicated (Diagenode; 25 cycles, 30 sec on and off). Sheard chromatin was diluted to achieve 0.1 % SDS and incubated with FOXA1 antibody (Novus Biologicals ...
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bioRxiv - Genomics 2019Quote: ... at 0.3J/cm2, and then lysed (1% SDS, 50mM Tris pH7.5, 500 mM NaCl) and sonicated using a Bioruptor (Diagenode) (six cycles 30” on 45” off) ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... 1:100) followed by sonication for 16 cycles (bursts and intervals of 30s each) with the Bioruptor® (Diagenode), which produced 200-1000 bp genomic DNA fragments ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Cell Biology 2021Quote: ... and for 15 minutes at 100°C in a proteinase K/elution buffer mix (1 µl of proteinase K in 100 µl DIB-buffer; Diagenode). Capture efficiency was determined by qPCR against spiked-in Lambda-DNA fragments in precapture and postcapture library samples ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Neuroscience 2022Quote: ... cells were cross-linked with 1% paraformaldehyde for 15 minutes and Chromatins were sheared into an average 200–400 bp in length by sonication (Diagenode) and immunoprecipitated with following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rested for 1 min on ice and sonicated (20 cycles: 15 seconds on/off; Bioruptor plus model UCD-300, Diagenode). Reduction and alkylation of disulfide bridges was performed by incubating the samples at 95 °C for 5 minutes ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Genetics 2020Quote: S2 cells transfected with either mCherry-Nnk or Odj-mCherry for 72 hours were fixed with 1% paraformaldehyde for 10 minutes and sheared with Bioruptor sonicator (Diagenode) to obtain chromatin ...
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bioRxiv - Molecular Biology 2019Quote: ... The digestion was then stopped with 5mM EDTA and the digested chromatin was solubilized in 1% SDS and three pulses of 30sec sonication using a Bioruptor ultrasonic cell disruptor (Diagenode). 200µg of pre-cleared chromatin was immunopurified with incubation of 30µl of Anti-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Molecular Biology 2019Quote: ... SDS was then added to a final concentration of 1% followed by a 3x 30sec sonication steps with a bioruptor sonicator (Diagenode). Insoluble precipitates were removed by centrifugation and soluble crosslinked chromatin extracts were diluted 10x in IP buffer (0.3M Sucrose ...
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bioRxiv - Neuroscience 2020Quote: ... PFFs are diluted in PBS and sonicated for 10 cycles (1 sec ON, 30 sec OFF, high intensity) in a bath sonicator at 10°C (BioRuptor; Diagenode).
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were cross-linked for 10 min at room temperature with 1% formaldehyde before lysis followed by sonication of their chromatin (Bioruptor Plus, Diagenode) to obtain DNA fragments of 200–400 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were lysed in NLB (50 mM Tris-HCl pH8, 10 mM EDTA, 1% SDS and 1x Proteases Inhibitor cocktail. Chromatin was sonicated using a Diagenode Bioruptor (40 Hz ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Cancer Biology 2019Quote: ... 1 × 107 cells / immunoprecipitation were fixed for 30 minutes (min) at room temperature (RT) using Diagenode Crosslink Gold (Diagenode, Liège, Belgium). After two washing steps with PBS (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA (1 μg) was fragmented to 300–600 bp after 25 cycles of 30 s of pulsed sonication in Bioruptor (Diagenode, Belgium). Fragmented DNA was end-repaired and adapter ligation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 mM PMSF) and chromatin was sheared by sonication to approximately 500-100 bp fragments using the Bioruptor® sonicator (Diagenode, Belgium). Immunoprecipitation was done with the anti-H3K27me3 (Active-motif ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... The membranes were incubated with blocking buffer (5% milk powder in TBST) for one hour at room temperature and afterwards incubated with primary antibody (anti-H3K27me3, Diagenode # C15410195, 1:1000) in blocking buffer overnight at 4 °C ...