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Citations for Diagenode :
1 - 50 of 455 citations for Rat Starch Binding Domain Containing Protein 1 STBD1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... incubated in blocking buffer with 1:500 rat anti-5hmC (Diagenode) overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734 ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Developmental Biology 2020Quote: ... The worm suspension containing fixed DNA-protein complexes was sheared using a Bioruptor (Diagenode, Denville, NJ) on the high setting for 20 cycles (30 seconds ON/30 seconds OFF) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... The Low Cell# ChIP kit protein A × 48 was from Diagenode (Denville, NJ, USA) and the Chemiluminescence western blot detection reagent was from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of EdU-containing DNA was performed using the iDeal ChIP-seq kit for Transcription Factors (Diagenode) with the following modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... 1.2 μg total protein was analysed with Cas9 (Diagenode C15310258, 1:400) and GAPDH (EMD MAB374 ...
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bioRxiv - Systems Biology 2023Quote: ... ∼400 ng DNA-containing chromatin was incubated with 1 µg antibody (H3K9/14ac (Diagenode, C15410005); H3K9me2 (in house ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ChIP-seq experiments were carried out using the HighCell# ChIP-Protein G kit (Diagenode, Cat#: C01010063) following the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... the pellets were resuspended in 300 μl ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON–60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The remaining pellet was re-suspended in 300 µl of lysis buffer containing 1% SDS and sonicated using a bioruptor (Diagenode) at 4°C (15 sec ON/OFF for 5-min and repeated for 5 cycles) ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet containing the nuclei was then resuspended in TBS containing 0.1% SDS and sonicated using Bioruptor Pico (Diagenode) on at 30 seconds on–30 seconds off for 10 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... Each chromatin sample containing about 6,000 cells was incubated with 1 µg of anti-H3K27me3 antibody (Diagenode, C15410195, lot. A1811-001P). ChIP was performed using the automated platform SX-8G IP-Star platform (Diagenode ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
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bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
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bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
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bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
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bioRxiv - Genetics 2023Quote: ... 0.1 % SDS) containing Complete proteinase inhibitor cocktail and sonicated with Picoruptor (Diagenode). After sonication debris was removed by centrifugation at 20,000 g for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Libraries were generated from 1 ng of DNA using the MicroPlex Library Preparation Kit v2 (C05010012, Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
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bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein extracts were finally sonicated in a Bioruptor (Diagenode) for 3 cycles at high intensity ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was transferred to a fresh tube containing H3K4me3 antibody (Diagenode Catalog# 15410003) bound protein G Dynabeads that were washed and resuspended in 100 µL of combined buffer ...
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bioRxiv - Neuroscience 2023Quote: ... in presence of 50 mg Protein Extraction beads (C20000021, Diagenode); samples were trypsin digested for 2 h 30 min ...
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in RIPA Buffer containing complete protease inhibitors (EDTA-free) and sonicated (Bioruptor, Diagenode). Cellular debris was pelleted at 10 000 rpm for 10 min at 4°C and protein concentration determined ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by immunoprecipation of immunocomplexes with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 1 hr at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Sheared chromatin was thereafter immunoprecipitated with Protein A-coated magnetic beads (Diagenode) preincubated with 4.5 μg/IP MED1 antibody (Bethyl A300-793A ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...