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Citations for Diagenode :
201 - 250 of 302 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Ribosome profiling sequencing libraries were prepared using the D-Plex Small RNA-seq kit (C05030001, Diagenode) with slight modifications ...
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bioRxiv - Cancer Biology 2021Quote: ... IP was carried out using the 3µg of polyclonal CTCF antibody (Diagenode, C15410210). DNA from protein-associated complexes and corresponding input samples were washed ...
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bioRxiv - Developmental Biology 2021Quote: ... The antibodies used were IgG as negative control (2μl, Diagenode #kch-819-015), anti-Histone3 as positive control (2μg ...
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bioRxiv - Synthetic Biology 2021Quote: ... with following antibodies at 4 °C on a rotating wheel: H3K27me3 (Diagenode, C15410195), Ring1B (Cell Signaling ...
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bioRxiv - Genomics 2023Quote: ... following the manufacturer’s protocol with a mouse antibody against 5hmC (Diagenode, C15200200-50) or 5mC (Diagenode ...
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bioRxiv - Genomics 2024Quote: ... Sheared chromatin was immunoprecipitated overnight with the following antibodies: H3K4me3 (Diagenode – C1540003-50), H3K27ac (Abcam – ab4729) ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154 ...
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bioRxiv - Neuroscience 2022Quote: ... A modified protocol for on-beads digest (sonication for 10*30s in a Bioruptor sonicator in presence of 50 mg Protein Extraction beads, C20000021, Diagenode) was used for tissue samples followed by a 3.5h digestion step ...
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bioRxiv - Molecular Biology 2020Quote: ... and RISC-Poor small sequencing libraries were constructed with CATS RNA-seq kit (Diagenode C05010041 Lot#4). Library primer dimers were removed by AMPureXP beads (Beckman) ...
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bioRxiv - Plant Biology 2021Quote: ... anti-GT1 (1:75) and anti-YSPTSPS repeat S2Pho (RNA Pol II, B1 subunit; 1:200, Diagenode). The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... Ribosome profiling libraries were prepared as previously described41 using the D-Plex Small RNA-Seq Kit (Diagenode). The cDNA was amplified for ten cycles ...
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bioRxiv - Cell Biology 2019Quote: ... Immunoprecipitation was performed by incubating 2µg of H3K4me3 antibody (Diagenode pAb-030-050 - C15410030) or IgG (sc-2025 ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by immunostaining with Cas9 monoclonal antibody 4G10 (Diagenode cat#: C15200216; 1:200 dilution) with gentle shaking for 2h at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... The following antibodies were used for western blot analysis: Cas9 (Diagenode C15310258, 1:1000) TCF4 (Abcam ab217668 ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies against MBD1 (pAb-078-050) and UHRF1 (H00029128-B01P) were purchased from Diagenode and Abnova ...
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bioRxiv - Genetics 2022Quote: ... for immunoprecipitation with 2μg of the following rabbit primary antibodies: anti-H3.3 (Diagenode, C15210011), anti-panH3 (Abcam ...
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bioRxiv - Genetics 2020Quote: ... Mouse chromatin was ChIPped using the following amounts of antibody: 1µg H3K4me3 (Diagenode C1540003), 0.2µg H3K4me1 (Diagenode C1540194) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Samples were then incubated for 14 hours with the following antibodies: H3K27ac (Diagenode, C15410196), H3K27me3 (abcam ...
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bioRxiv - Developmental Biology 2024Quote: ... The supernatant was transferred to a fresh tube containing H3K4me3 antibody (Diagenode Catalog# 15410003) bound protein G Dynabeads that were washed and resuspended in 100 µL of combined buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Developmental Biology 2019Quote: ... Embryos were kept on ice and processed directly or frozen at −80C in 20 ml of RNAse/DNAse free PBS supplemented with 1× protein inhibitor (Diagenode, Belgium). Embryos younger than late gastrula (younger than E7.5 ...
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bioRxiv - Genetics 2021Quote: ... 3 to 7 days later individual colonies were picked and expanded and western blot was used to confirm Cas9 protein expression (Diagenode, C15200203). Cas9 expression in a high expressing line was confirmed by immunohistochemistry (5).
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 30 µg of chromatin and 4 μl of mouse anti-ERalpha monoclonal antibody (C15100066, Diagenode) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... ∼400 ng DNA-containing chromatin was incubated with 1 µg antibody (H3K9/14ac (Diagenode, C15410005); H3K9me2 (in house ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196 ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Developmental Biology 2021Quote: ... To prepare the RNA–seq libraries the amplified cDNA (2μg per sample) was fragmented to 200bp using a Bioruptor Sonicator (Diagenode), end repaired and barcoded using the Ovation Rapid DR Library System (NuGEN) ...
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bioRxiv - Plant Biology 2023Quote: ... rRNA depleted RPFs were directly used as input for the D-plex small RNA-seq kit (Diagenode cat#C05030001), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... ChIP-seq was performed using the protocol previously described (51) with antibodies to H3K27Ac (C15410196, Diagenode) and H3K27me3 (9733S ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Molecular Biology 2022Quote: ... chromatin of each replicate was incubated with 1 μg of antibody against H3K27ac (Diagenode, C15410196, RRID:AB_2637079) or 1 μg of antibody against ONECUT2 (Proteintech ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...
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bioRxiv - Cancer Biology 2024Quote: ... 25µg of sheared chromatin was then incubated with 4µg of antibody against H3K4me3 (Diagenode, C15410003-50) H3K36me3 (Abcam ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 500 ng of total RNA was used for NGS libraries preparation with CATS mRNA-seq kit (Diagenode, Liege, Belgium) according to the manufacturer’s recommendations ...
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bioRxiv - Genetics 2020Quote: ... 500 ng of each sample was converted into a sequencing-ready library using the CATS RNA-seq kit with rRNA depletion (Diagenode). The RNA integrity ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Cancer Biology 2024Quote: ... p300, ERG, BRD4 and MED1 ChIP-seq) or Histones (for H3K27ac, H2BK5ac, H3K20ac, and RNA Pol II ChiP-seq) (Diagenode) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: The HiChIP protocol was performed as previously described (Mumbach 2016) using 7.5µg antibody to H3K27ac (Diagenode, C15410196) with the following modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... 3µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Genetics 2020Quote: ... that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50, Diagenode) or polyclonal H3K9me3 antibody (AB_2532132 ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...