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Citations for Diagenode :
51 - 100 of 109 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... Nuclei were resuspended in Nuclei Lysis Buffer and chromatin was sonicated using a water bath Bioruptor Pico (Diagenode; 30 s on / 30 s off pulses ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Cancer Biology 2022Quote: ... The pellet was resuspended in 400µL of lysis buffer (1% SDS, 50mM Tris-HCl pH 7.5) and sonicated with the Pico Bioruptor (Diagenode) for 20 cycles (30sec ON/ 30sec OFF ...
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bioRxiv - Plant Biology 2020Quote: ... Beads were incubated for 10h with 50 µL of sheared chromatin diluted in ChIP dilution buffer (Diagenode, Belgium). One tenth of the diluted chromatin was collected and kept aside as an input sample before incubation with antibody ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were resuspended in Nuclei Lysis Buffer and chromatin was sonicated using a water bath Bioruptor Pico (Diagenode; 30 s on / 30 s off pulses ...
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bioRxiv - Molecular Biology 2024Quote: ... The chromatin pellet was resuspended in FA lysis buffer / 0.1% SDS and sheared with a Bioruptor sonicator (Diagenode) at 15 s ON / 45 s OFF for 80 min ...
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bioRxiv - Systems Biology 2023Quote: ... The collected tissue slices were sonicated in the lysis buffer with the non-contact sonication using Bioruptor (Diagenode) for 20 cycles (30 s-on ...
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bioRxiv - Plant Biology 2024Quote: ... Nuclei were resuspended in Nuclei Lysis Buffer and chromatin was sonicated using a water bath Bioruptor Pico (Diagenode; 30 s on/30 s off pulses ...
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bioRxiv - Cancer Biology 2023Quote: ... ATAC-seq library preparation involved PCR amplification of the purified DNA fragments using Diagenode primer indices (Diagenode, Cat# C01011034). Amplification cycles were adjusted and libraries were purified using AMPure beads ...
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bioRxiv - Molecular Biology 2019Quote: ... The pellet was then resuspended in 600 µl FA lysis buffer using a syringe and a 23G needle and split over 2x 1.5 ml bioruptor pico microtubes (Diagenode). The chromatin was sheared in a bioruptor pico sonicator (Diagenode ...
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bioRxiv - Plant Biology 2021Quote: ... The pellet was resuspended in 300 μl of nuclei lysis buffer and sonicated with a BioRuptor Pico sonicator (Diagenode) for 26 to 36 cycles to obtain an approximately 200 bp fragment distribution ...
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bioRxiv - Microbiology 2020Quote: ... cells were lysed in buffer A (6M guanidium-HCl, 0.1M Na2HPO4/NaH2PO4, 10mM imidazole, pH 8.0) and sonicated with a Bioruptor™ (Diagenode) (10 cycles ...
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bioRxiv - Microbiology 2020Quote: ... 10-20 ng/μL of DNA extract in 100 µL chilled TLE buffer (10mM Tris, pH8.0, 0.1mM EDTA) was sheared using a Bioruptor™ (Diagenode) on high power for up to 15 min ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL resuspension buffer (50 mM HEPES-NaOH pH 7.2, 150 mM KCl) and lysed for 10 minutes using the Bioruptor (Diagenode, 10 cycles ...
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bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
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bioRxiv - Cancer Biology 2020Quote: ... which where lysed in a high-salt buffer (50mM Tris, 600mM NaCl, 10% glycerol, 0.2% NP40, 0.5mM DTT) followed by sonication to fragment chromatin (Diagenode Bioruptor). Buffers were supplemented with phosphatase and Complete ULTRA protease inhibitor cocktails (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were harvested in lysis buffer containing 0.1% Rapigest and sonicated (10 cycles of 30 seconds on, 30 seconds off in Bioruper, Diagenode). Approximately 30 μg of protein was resolved on a 4-20% Miniprotean TGX gel ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were taken up in 100 μL LYSE buffer and mixed with 50 mg of protein extraction beads (Diagenode). Samples were sonicated with 10-20 cycles at 30sec ON/OFF in a Diagenode Bioruptor Plus until complete tissue disruption was observed ...
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bioRxiv - Neuroscience 2024Quote: ... 350 μL ice-cold TE buffer was added and chromatin was sheared in 1.5 mL microtubes using a Bioruptor Pico sonication device (B01060010, Diagenode) for 3 × 5 cycles (30 s ON/ 30 s OFF ...
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bioRxiv - Molecular Biology 2024Quote: ... the insoluble pellet was resuspended in RIPA buffer (Cold Spring Harbor Protocol) and followed by 15min sonication by Diagenode bioruptor with medium power for 30s on and 30s off at 4 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinked samples were washed in phosphate buffered saline (PBS), resuspended in ChIP lysis buffer (0.5% SDS, 10mM EDTA, 50mM Tris-HCl pH8) and sonicated (Bioruptor Plus, Diagenode) to release 100-1000 bp fragments ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Microbiology 2020Quote: ... supernatant aspirated and suspended in chromatin shearing buffer for sonication (10 cycles of 15 sec ‘on’ and 30 sec ‘off’) with a Bioruptor (Diagenode) to 200-900bp size ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in radioimmunoprecipitation assay (RIPA) buffer containing protease and phosphatase inhibitor cocktails and sonicated for 1 min with the Bioruptor sonication device (Diagenode). Cell lysates were centrifuged at 13,000 × g for 10 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed with RIPA lysis buffer with protease inhibitors on ice for 15 minutes and then sonicated with a Bioruptor (Diagenode) for 10 minutes on high with 30 seconds on and 30 seconds off ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were lysed in 1 ml extraction buffer by three freeze-thaw cycles using liquid nitrogen and by sonication using a Bioruptor (Diagenode) with one 30 s high power pulse ...
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bioRxiv - Genetics 2019Quote: 0.5 × 106 frozen cells were resuspended in 250 µl buffer iL1 with proteinase inhibitors cocktail (iDeal ChIP-seq Kit for Histones, Diagenode) and incubated for 10 min at 4ºC on the Bohemian wheel ...
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in 1 mL of FA lysis buffer and subjected to DNA sonication for 20 cycles of 30 secs in a Bioruptor Twin (Diagenode). Following pelleting by 15 min centrifugation at 13000 rpm ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Cell Biology 2020Quote: ... the lysate was diluted 10 times with lysis buffer and subjected to sonication for 15 min in a refrigerated water bath (Bioruptor, Diagenode). Part of the sonicated chromatin was treated with proteinase K and RNase A and analyzed by electrophoresis in a 1.5% agarose gel to control the size of the DNA fragments (200-500 bp) ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was spun at 13,000 rpm for 30 min at 4°C and the pellet was resuspended in 300 μl ChIP lysis buffer + 1mM PMSF and sonicated for 15 min (30 sec ON - 60 sec OFF) in a Bioruptor (Diagenode). The lysate was spun at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended in 2mL cell isolation buffer (IBc) in a 15mL conical and disrupted using a Bioruptor sonicator (Diagenode) with metal probe adaptor and short one-second pulses on “HI” setting ...
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bioRxiv - Genetics 2023Quote: ... third instar larval wing discs were prepared by boiling samples for 10 minutes in Laemmli SDS-PAGE loading buffer followed by sonication using the Bioruptor Pico sonication system (Diagenode) for 10 cycles (30 sec on ...
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bioRxiv - Cell Biology 2023Quote: ... the pellet was washed with lysis buffer and sonicated to a shearing size of <500 nucleotides using a bath sonicator (Biorupter XL; Diagenode). The sonicated material was centrifuged ...
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bioRxiv - Cell Biology 2023Quote: ... the pellets were resuspended in 300 μl ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON–60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... The remaining pellet was re-suspended in 300 µl of lysis buffer containing 1% SDS and sonicated using a bioruptor (Diagenode) at 4°C (15 sec ON/OFF for 5-min and repeated for 5 cycles) ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluate was transferred to a new tube containing 100 μl of TE buffer and then incubated with proteinase K (Diagenode) at 55 °C for 1 h ...
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bioRxiv - Microbiology 2023Quote: ... The pellet corresponding to the nuclear fraction is dissolved in 300 µL of 1x SDS loading buffer with 100 mM DTT and sonicated with Bioruptor (Diagenode) 5 cycles of 30 seconds ON 30 seconds OFF intervals at high intensity ...
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bioRxiv - Cancer Biology 2023Quote: ... were lysed in buffer (50 mM Tris-HCl, pH 7.8) containing 1% SDS and 0.1 M dithiothreitol and sonicated (Diagenode Bioruptor Plus). Number of sonication cycles was established based on a visual sample clarity assessment ...
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bioRxiv - Neuroscience 2023Quote: ... Cell pellet was resuspended in ice-cold lysis buffer (1xPBS, 2 mM PMSF, 2 mM DTT, 1 x protease inhibitors (Pierce)) and sonicated (Bioruptor Pico, Diagenode) at 4LJ with alternating 10 s burst/10 s break ...
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bioRxiv - Cancer Biology 2024Quote: ... cells pellets were then resuspended in modified RIPA buffer (50nm Tris pH 7.5, 150nM NaCl, 5mM EDTA, 50nM NaF, both inhibitors) and sonicated using Bioruptor® Pico (Diagenode). To remove the possibility of any DNA-dependent protein interactions ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Developmental Biology 2022Quote: ... Total protein was extracted by incubating the islets with Laemmli buffer at 95°C for 5 mins and homogenized for another 5 mins in a sonication device (Diagenode, B01060001) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 50 µL storage buffer was added to chromatin and samples were transferred to 1.5 mL Bioruptor Microtubes with Caps for Bioruptor (Diagenode, Denville, NJ). Samples were then loaded into Pico Biorupter (Diagenode ...