Labshake search
Citations for Diagenode :
1 - 50 of 109 citations for PCR Buffers since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... iW3 and iW4 buffer (Diagenode). ChIP-DNA was then eluted ...
-
bioRxiv - Molecular Biology 2019Quote: ... The pellet was washed with 1 ml lysis buffer and gently resuspended in 300 µl lysis buffer containing 0.2% SDS and sheared by sonication with Bioruptor (Diagenode) for 30 minutes (30s On ...
-
bioRxiv - Cell Biology 2021Quote: ... and for 15 minutes at 100°C in a proteinase K/elution buffer mix (1 µl of proteinase K in 100 µl DIB-buffer; Diagenode). Capture efficiency was determined by qPCR against spiked-in Lambda-DNA fragments in precapture and postcapture library samples ...
-
bioRxiv - Cell Biology 2023Quote: ... then resuspended in 100 μl buffer iE1 (Diagenode) and incubated rotating for 30 min at room temperature before removing the beads ...
-
bioRxiv - Cell Biology 2021Quote: ... Samples were mixed with IP buffer (MagMeDIP –kit; DIAGENODE) and denatured for 3 minutes at 95°C ...
-
bioRxiv - Molecular Biology 2020Quote: ... resuspended in buffer and sonicated using the Bioruptor (Diagenode, Belgium) followed by a cup-horn (Branson ...
-
bioRxiv - Cancer Biology 2022Quote: ... lysed using ChIP Lysis Buffer and sonicated using Bioruptor (Diagenode) as described above ...
-
bioRxiv - Molecular Biology 2019Quote: The protocol and buffers from the True MicroChIP kit (C01010130, Diagenode) were used to perform sonication and immunoprecipitation (IP ...
-
bioRxiv - Molecular Biology 2022Quote: ... incubated in blocking buffer with 1:500 rat anti-5hmC (Diagenode) overnight at 4°C and followed by incubation with 1:1000 anti-rat IgG HRP (ab6734 ...
-
bioRxiv - Physiology 2023Quote: ... Samples were lysed in RIPA buffer using Bioruptor Sonication System (Diagenode) for 20mins ...
-
bioRxiv - Neuroscience 2023Quote: ... 2xTD buffer and Tn5 transposase were commercially available (Diagenode C01019043, C01070012). Reactions were incubated at 37C for 30min without agitation ...
-
bioRxiv - Genomics 2019Quote: ... Resultant PCR products were sheared to ∼400bp using the Bioruptor Pico (Diagenode) and Nextera XT indexes added as previously described29 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Chromatin was sheared in Shearing buffer iS1 using the Bioruptor Plus (Diagenode) for 20 cycles (each cycle for 30 seconds “ON” and 45 seconds “OFF” ...
-
bioRxiv - Genomics 2020Quote: ... followed by 20 min wash cycle using the provided wash buffers (DIAGENODE Immunoprecipitation Buffers ...
-
bioRxiv - Cell Biology 2019Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a QuantStudio 6 instrument (Themo Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... cDNA was quantified by real-time PCR using SYBR Green Master Mix (Diagenode) on a Quant Studio 5 384 well plate ...
-
bioRxiv - Cancer Biology 2019Quote: ... Cells were lysed in SDS lysis buffer followed by sonication in Bioruptor (Diagenode) to obtain 200-1000 bp chromatin fragments ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples in lysis buffer were boiled for 10 minutes and sonicated (Bioruptor, Diagenode) for 15 minutes at room temperature ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...
-
bioRxiv - Physiology 2023Quote: ... The peptides were resuspended in immunoaffinity purification (IAP) buffer and sonicated (Bioruptor, Diagenode) for 15 min Protein concentration was determined by Pierce BCA Protein Assay (Thermo) ...
-
bioRxiv - Genomics 2023Quote: ... the nuclei pellet was resuspended in 1mL of shearing buffer + protease inhibitor (Diagenode), incubated for 10 mins on ice ...
-
bioRxiv - Genomics 2023Quote: ... followed by a 20 min wash cycle using the provided wash buffers (Diagenode Immunoprecipitation Buffers ...
-
bioRxiv - Microbiology 2020Quote: ... Purified PCR products were sheared by sonication using a Picoruptor device (Diagenode, Liege, Belgium) to obtain fragments with an average size of 300–400 bp ...
-
bioRxiv - Cancer Biology 2022Quote: ... and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
-
bioRxiv - Developmental Biology 2022Quote: ... Chromatin pellets were then sonicated in 450 μl buffer A2 using a Bioruptor (Diagenode) for 16 min (30 s on/30 s off) ...
-
bioRxiv - Neuroscience 2019Quote: ... Nuclei diluted in 1 ml RIPA buffer were sonicated using Bioruptor (Diagenode, Denville, NJ) for 6 cycles of 5 minutes (60 seconds on/30 seconds off ...
-
bioRxiv - Genetics 2019Quote: ... 2 μl of IP material were resuspended in 30 ul ChIP-seq buffer (Diagenode) supplemented with 1 μl Tn5 (Illumina) ...
-
bioRxiv - Genomics 2021Quote: ... cells were lysed by SDS Lysis Buffer and sonicated for 1.5 hrs (Diagenode Bioruptor) at 4°C on the high setting with 30 seconds on/off interval ...
-
bioRxiv - Genomics 2020Quote: ... 73 µl of 1X buffer iC1 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) supplemented with Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Cancer Biology 2020Quote: ... Fixed cells were washed and resuspended in sonication buffer supplemented with protease inhibitors (from Diagenode) and PhosStop (Roche) ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell pellets were lysed in 300 μl of RIPA buffer and sonicated with Bioruptor (Diagenode; number of cycles = 15 ...
-
bioRxiv - Genomics 2023Quote: ... 6μL DNA extract were incubated (55°C) with 5μL of 2X Tagmentation Buffer (Diagenode, C01019043) and 1μL of Loaded Tn5 Tagmentase (Diagenode ...
-
Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... and sodium lauroyl sarcosinate in RSB buffer and were sonicated for 10 min (Diagenode Bioruptor). Extracts were then diluted (1:4 in RSB + T buffer ...
-
bioRxiv - Evolutionary Biology 2023Quote: PCR products for each sample were pooled at equimolar ratios and sonicated using a Picorupter (Diagenode) to shear the DNA into small fragments (seven cycles of 15 sec on and 45 sec off) ...
-
bioRxiv - Neuroscience 2023Quote: ... Real Time qPCR was performed by using a real-time PCR SYBR green master mix (Diagenode). Samples were run and analyzed on a Quantstudio 5 (Applied Biosystems) ...
-
Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The eluates were diluted 1:50 with ChIP buffer from the One Day ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the One Day ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C ...
-
bioRxiv - Genetics 2019Quote: ... The cells were then sonicated in buffer iS1 using the Bioruptor® Pico sonication device (Diagenode) to achieve fragment sizes distribution below 3 kb.
-
bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... Centrifuged cell lysates were resuspended in shearing buffer and sonicated using the Pico Bioruptor device (Diagenode) for 15 minutes (30s ON/30s OFF) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in RIPA Buffer containing complete protease inhibitors (EDTA-free) and sonicated (Bioruptor, Diagenode). Cellular debris was pelleted at 10 000 rpm for 10 min at 4°C and protein concentration determined ...
-
bioRxiv - Molecular Biology 2021Quote: ... The resulting chromatin was sheared in NP buffer using a sonicator bath (Bioruptor Pico; Diagenode, Ougrée, Belgium) for 30 minutes at maximum output and 30 seconds on/off cycles ...
-
bioRxiv - Genomics 2020Quote: ... at 1g of chromatin per 3.2mL of buffer and sonicated on a Bioruptor NextGen sonication device (Diagenode) to shear the chromatin to an average fragment size of 200 - 500 bp as determined by agarose gel electrophoresis ...
-
bioRxiv - Cancer Biology 2022Quote: ... and the nuclei were resuspended in 50 µL transposition mixture (25 µL 2× Tagmentation buffer (Diagenode, C01019043), 2.5 µL Tagmentase loaded (Diagnode ...
-
bioRxiv - Cancer Biology 2023Quote: ... The pellets were resuspended in 300 μL Lysis Buffer 3 and sonicated by a Bioruptor Pico (Diagenode), resulting in chromatin fragments of around 300 to 500 bps ...
-
bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043), 0,1% Tween-20 ...
-
bioRxiv - Microbiology 2024Quote: ... The Mock (15 ng in 20 µl TE buffer) was randomly fragmented by BioRuptor (Diagenode, Liege, Belgium) at default intensity for 15 sec on and 90 sec off ...
-
bioRxiv - Developmental Biology 2022Quote: ... To make sequencing libraries the PCR enriched genomic DNA sample was sonicated using a Bioruptor bath sonicator (Diagenode), purified using AMPure XP beads ...
-
bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
-
bioRxiv - Molecular Biology 2021Quote: ... sample DNA was ethanol precipitated and sheared in 500 µL sonication buffer (50 mM Tris pH 8.0, 0.1% SDS, 10 mM EDTA) on a Bioruptor Pico sonicator (Diagenode). DNA was then concentrated on Amicon ultra 0.5 30K filter units (Millipore) ...