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Citations for Diagenode :
101 - 150 of 395 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: ... The extracts were then distributed into 1.5 ml reaction tubes and sonicated in an ice bath four times for 30 seconds on high setting using a Bioruptor UCD-200 (Diagenode) with 1.5-minute breaks on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Neuroscience 2022Quote: ... sample was sonicated for 18 cycles on high power setting (30s ON, 30s OFF) using the Bioruptor Next Gen (Diagenode) and then centrifuged at 20.000xg for 10 min at 4°C.
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated for 10-15 cycles of 30s ON/30s OFF on high power using the Bioruptor Plus (Diagenode), then diluted in additional lysis buffer 3 and clarified by centrifugation for 10 min at max speed at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (10 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300), complete lysis was monitored by phase contrast microscopy ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then subjected to five cycles (30 seconds on/30 seconds off) of sonication at 4°C on high intensity in a Bioruptor Sonication System (Diagenode) and subsequently cleared by centrifugation at 14,000g for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... was added per sample to digest cell walls and samples were incubated for another 15 min at 4°C before sonicated in an ice bath 4x for 30 s on high setting using a Bioruptor UCD-200 (Diagenode) with 90 sec breaks on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... and sonicated (6 cycles, high power for 30 s on and 30 s off at 4 °C, Bioruptor NextGen (Diagenode)) ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: ... processed using the TissueLyser for 2 times for 1min going from 3 to 30hz and sonicated on high for 5x 60 seconds on and 30 seconds off in a Bioruptor Pico sonication water bath (Diagenode) at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1 % SDS in PBS) and sonicated 5 times for 5 min (30s ON/ 30s OFF) at high mode in a Bioruptor Pico (Diagenode). IP were performed overnight at 4°C using 1.5 mg of pre-coated epoxy Epoxy Dynabeads (M-270 ...
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bioRxiv - Molecular Biology 2024Quote: Multiple ChIP experiment samples were pooled and sonicated for 15 cycles (30 sec on, 30 sec off, high setting) with a Bioruptor (Diagenode), then concentrated with a vacuum (Eppendorf) ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Cell Biology 2021Quote: ... To shear any remaining nucleic acids, samples were sonicated in high mode (30 sec OFF, 30 sec ON) for 12 cycles (Bioruptor® Plus; Diagenode). Then ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: Membranes disruption was carried by sonication at 4 °C (2x10 min, 30 sec on, 30 sec off, high setting, Diagenode Bioruptor). Extracts were cleared by centrifugation (15,000 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... in 10 µl nuclease-free H2O and amplified with NEBNext® High-Fidelity 2X PCR Master Mix and 1 ul of primer pair from Diagenode (24 SI for tagmented libraries ...
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bioRxiv - Genomics 2023Quote: ... Samples were sonicated for 10-12 minutes (high intensity, 30s on/30s off) in a Bioruptor Pico system (Diagenode, Denville, NJ) at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% Tween-20) and lysed by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Cell lysates were subjected to tandem affinity purification as described above ...
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bioRxiv - Cancer Biology 2023Quote: ... to pellet cellular debris and the collected supernatant was sonicated on high power for 20 cycles each of 30 second on/off pulses using a Bioruptor® sonication system (Diagenode Inc ...
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... After glass-bead beating, chromatin was sonicated for 24 cycles (high output, 30 s-on, 30 s-off) using the Bioruptor Plus (Diagenode, Belgium) to produce fragments of ∼ 250 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Chromatin was sonicated in Shearing Buffer (0.1% SDS, 1mM EDTA and 10mM Tris pH 7.5) using Bioruptor sonicator (Diagenode; high power setting) to obtain fragments of about 500-800 bp ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Immunology 2022Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Genomics 2019Quote: ... MethylCap kit (Diagenode, C02020010) was employed to obtain the methylated DNA ...
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bioRxiv - Immunology 2021Quote: ... iPure kit v2 (Diagenode) was used ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Cancer Biology 2021Quote: ... the lysate was then sonicated on the high setting for a total of three 30 second cycles using the Bioruptor® Plus (Diagenode Inc.). For SDS-PAGE electrophoresis ...
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bioRxiv - Immunology 2022Quote: ... and was sonicated for 4 rounds of 15 cycles of 30 seconds on/30 seconds off on high power (Diagenode Biorupter plus). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin lysate was sonicated to an average size of ~250 bp using 40 cycles of sonication (30 sec on/off High-Power setting; Diagenode Biorupter Plus). A 20% fraction of the sonicated chromatin was incubated with one of the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified DNA was fragmented to obtain fragments of an average size of 300–400 bp using a Bioruptor Pico (Diagenode; 8 cycles; 20 sec ON/60 sec OFF). 3 μg of DNA per condition were used for library preparation ...
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bioRxiv - Cancer Biology 2021Quote: ... Then the solution was sonicated at 4 °C for 6 cycles (1cycle = 30 s sonication and 30 s cooldown with “High” energy intensity) using a Bioruptor® Plus sonication (Diagenode, UCD-300). After sonication ...
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bioRxiv - Microbiology 2022Quote: ... resuspended in 1 ml PBS and sonicated at high intensity for 5 min in 30 s on 30 s off cycles using a Bioruptor (Diagenode, Denville, NJ, USA). Protein was determined in all samples by the Bradford method and a known mass of sample was mineralized in concentrated HNO3 trace metal grade (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... Auto hMeDIP kit (Diagenode C02010033) was used according to manufacturer instructions ...
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bioRxiv - Immunology 2019Quote: ... using LowCell# ChIP kit (Diagenode), with 1μg of antibody against ARNTL (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... or hMeDIP kit (Diagenode, C02010031). Quantification of precipitated DNA was determined using real-time PCR and the delta Ct method for relative quantitation (ABI 7900HT Fast Real-Time PCR System).
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% SDS and 0.5% N-lauroylsarcosine) and the chromatin was fragmented by sonication for 10 cycles on Bioruptor (Diagenode high settings, 30’’ on/off). Chromatin was separated from cell debris using centrifugation and stored at -80C for maximum of 2 months.
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...
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bioRxiv - Genetics 2020Quote: ... using the LowCell# ChIP kit (Diagenode). Illumina sequencing libraries were generated using the Accel-NGS 2S Plus DNA Library Kit (Swift Biosciences) ...
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bioRxiv - Developmental Biology 2022Quote: ... and the iDeal ChIP-kit (Diagenode) for MED1 according to the manufacturer’s protocols with some modifications as described below ...
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bioRxiv - Developmental Biology 2023Quote: ... the iDeal ChIP-Seq kit (Diagenode) was used according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... the µChipmentation kit for histones (Diagenode) was used for chromatin immunoprecipitation and ChIP-Seq library preparation (Supplementary table 2).
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bioRxiv - Genomics 2024Quote: ... using a Microplex v3 kit (Diagenode).
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...