Labshake search
Citations for Diagenode :
1 - 50 of 395 citations for Human High Sensitive Interleukin 8 IL8 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... and lysed by sonication at high power for 8 cycles (15 sec on, 60 sec off) using a Bioruptor (Diagenode). The lysates were collected after centrifugation at 13,000 rpm for 10 min at 4 °C and the supernatants were prepared for nuclear protein analysis ...
-
bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
-
bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
-
Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... high output (Diagenode Bioruptor). After centrifugation at 15,000 rpm for 10 min to collect the supernatants ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and chromatin was prepared using the Chromatin EasyShear Kit - High SDS (Diagenode; Cat# C01020012), and shearing of the chromatin was achieved using the Bioruptor with four rounds of ten cycles of 30 seconds “ON„ and 30 seconds “OFF„ at HIGH power setting (position H) ...
-
bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
-
bioRxiv - Biochemistry 2020Quote: ... at HIGH setting (Bioruptor Plus, Diagenode). Samples were centrifuged 10 min at 12,000 × g and 10% of the sample was saved as input DNA and stored at −80 °C until reversal of cross-linking ...
-
bioRxiv - Cancer Biology 2022Quote: ... Upon 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
-
bioRxiv - Microbiology 2021Quote: ... 30s OFF (setting high, BioruptorTM Next Gen, Diagenode) (Fraschka et al. ...
-
bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
-
bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
-
bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1mM EDTA) and subjected to 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
-
bioRxiv - Systems Biology 2022Quote: ... before high-energy sonication to shear DNA (Diagenode Bioruptor pico). The protein concentration was determined through a BCA assay (Thermo Fischer) ...
-
bioRxiv - Plant Biology 2021Quote: ... High-molecular-weight DNA was sheared with a Megaruptor (Diagenode) to obtain 15-20 kb fragments ...
-
bioRxiv - Genomics 2021Quote: ... The cells were lysed by high energy sonication (Bioruptor, Diagenode) and reduced by adding DTT (to a final concentration of 15 mM and heating for 30 minutes at 56 °C) ...
-
bioRxiv - Genetics 2021Quote: ... lysed at high performance with a Bioruptor Plus sonicator (Diagenode) using 10 cycles of 30 s sonication intervals ...
-
bioRxiv - Plant Biology 2022Quote: ... high molecular weight DNA was sheared with a Megaruptor (Diagenode) set to a 40 kb fragment size ...
-
bioRxiv - Genetics 2021Quote: ... Lysates were then sonicated at high intensity in a Bioruptor (Diagenode) water bath to shear DNA to desired size range (300-500 bp ...
-
bioRxiv - Cancer Biology 2022Quote: ... Lysates were sonicated at high power with a Bioruptor sonicator (Diagenode) for 15 min ...
-
bioRxiv - Microbiology 2023Quote: High molecular weight gDNA was sheared with the Megaruptor 3 (Diagenode) to obtain 10- 15 kbp fragments ...
-
bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
-
bioRxiv - Cancer Biology 2021Quote: ... Chromatin corresponding to 8 μg of DNA was incubated with anti-H3K9me3 (Diagenode; Cat#: C15410193), anti-H3K27me3 (Diagenode ...
-
bioRxiv - Plant Biology 2023Quote: ... and sonicated four times (BioruptorTM, Diagenode, 30s on/1min off on High). They were quantified at 205 nm using the Nanodrop 2000 according to the manufacturer’s instruction (Thermoscientific ...
-
bioRxiv - Cancer Biology 2024Quote: ... Nuclei were then sonicated at high energy in a Bioruptor Plus (Diagenode). Sonication parameters to produce DNA fragments in the 200-600 bp range were optimized beforehand ...
-
bioRxiv - Genomics 2024Quote: ... High molecular weight genomic DNA was sheared mechanically on Megaruptor 3 (Diagenode) to generate 30 Kb fragments ...
-
bioRxiv - Genetics 2019Quote: ... we used ChIP grade antibodies specific to human H3K27ac histones (Diagenode). A negative control undergoing no IP (input ...
-
bioRxiv - Systems Biology 2019Quote: ... Samples were further sonicated (15 cycles at high intensity using a Bioruptor, Diagenode) and boiled (90°C ...
-
bioRxiv - Cell Biology 2022Quote: ... with 30 sec breaks on high-performance mode with Bioruptor Plus (#B01020001, Diagenode). Samples were centrifuged at 20000 x g for 20 min at RT ...
-
bioRxiv - Cancer Biology 2019Quote: ... Twenty cycles of sonication (30s high, 30s off) using TPX tube (Diagenode, ref: 50001) and the Bioruptor (Diagenode ...
-
bioRxiv - Cancer Biology 2021Quote: ... and then sonicated for 5 min at high intermittent pulses (30/30) (Diagenode, Bioruptor). Samples were centrifuged for 10 min at 10,000 g at 4°C to remove any cell debris and typically 25 μg proteins were separated on SDS-polyacrylamide gels and analysed by Western blotting ...
-
bioRxiv - Genomics 2023Quote: ... in the high mode at 4°C in a Bioruptor water bath sonicator (Diagenode), to obtain fragments of 500 to 1000 bp in size ...
-
bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
-
bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
-
bioRxiv - Genomics 2022Quote: ... Each high-quality gDNA (200 ng) was fragmented by sonication using a Bioruptor (Fa. Diagenode) and subsequently used for library preparation with the TruSeq Nano DNA library preparation kit (Fa ...
-
bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
-
bioRxiv - Cell Biology 2020Quote: ... sonicated (5 cycles of 30 s ON, 30 s OFF, high power, Bioruptor Plus, Diagenode), spun for 10 min at 16,000 × gmax to remove any insoluble material ...
-
bioRxiv - Molecular Biology 2019Quote: ... Lysates were sonicated at medium-high intensity for 10 minutes in a Bioruptor Standard (Diagenode) placed at 4°C and subsequently boiled for 10 minutes at 90°C ...
-
bioRxiv - Genomics 2023Quote: ... and then high-quality genomic DNA was randomly fragmented using a Megaruptor (Diagenode, NJ, USA). High molecular weight (HMW ...
-
bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated for 15 min at high power with the Bioruptor sonicator (Diagenode). Protein quantification was performed using the Bradford reagent (Biorad #5000001) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated with 8 cycles of 30s with 30s intervals at the low setting in a Bioruptor Plus water bath sonicator (Diagenode) and centrifuged for 15 min at 16000 g at 4°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
-
bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
-
bioRxiv - Molecular Biology 2019Quote: ... mixed by rotation at 4°C for 30 min, and then sonicated for 8 cycles (ON for 30 s, OFF for 30 s) using a Picoruptor Sonicator (Diagenode). Each tube of lysate was incubated overnight at 37°C in hybridization buffer (750 mM NaCl ...
-
bioRxiv - Molecular Biology 2021Quote: ... ChIP from S2 cells was carried out using the same protocol from 2 x 107 cells with a modification to the chromatin preparation procedure to sonicate chromatin for 8 cycles of 30s on/30s off using a Bioruptor Pico (Diagenode). Recovered DNA was quantified on an Agilent TapeStation System using a High Sensitivity DNA ScreenTape (Agilent) ...
-
bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...
-
bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
-
bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
-
bioRxiv - Genomics 2020Quote: ... followed by sonication at high power for 10 cycles of 30s ON/OFF (Bioruptor Plus; Diagenode). Lysates were clarified by centrifugation at 10,000g for 15min ...