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Citations for Diagenode :
201 - 250 of 402 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 30 seconds off) for 7 cycles of sonication in a 4°C water bath using the Bioruptor Sonication System (Diagenode, Denville, NJ) at the high-intensity setting ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Biochemistry 2023Quote: ... and the air-dried pellets were resuspended in 500 μl 8 M urea and sonicated in a bioruptor (Diagenode) with high amplitude for 10 min with cycles of 30 seconds on and 30 seconds off.
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA was sheared by sonication for 15–24 min using a Bioruptor (Diagenode, Inc., New Jersey, USA). Size-selected samples (200–400 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were sonicated with 8 cycles of 30s with 30s intervals at the low setting in a Bioruptor Plus water bath sonicator (Diagenode) and centrifuged for 15 min at 16000 g at 4°C ...
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bioRxiv - Molecular Biology 2019Quote: ... treated by RNase A (20 µg/mL) for 1h at 37°C in TE buffer and sonicated 8 min with BioRuptor (Diagenode) (15 sec ON / 15 sec OFF ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... ChIP from S2 cells was carried out using the same protocol from 2 x 107 cells with a modification to the chromatin preparation procedure to sonicate chromatin for 8 cycles of 30s on/30s off using a Bioruptor Pico (Diagenode). Recovered DNA was quantified on an Agilent TapeStation System using a High Sensitivity DNA ScreenTape (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were suspended in the shearing buffer and sonicated for 8 cycles of 30sec on/off using Bioruptor Pico (Diagenode). The sheared chromatin was reverse-crosslinked and DNA fragment size was ensured by Agilent TapeStation ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Developmental Biology 2024Quote: ... were sonicated 8 pulses (30 seconds ON / 30 seconds OFF) to 200–500 bp fragments using a Bioruptor Pico sonicator (Diagenode) in 200ul of L3 buffer ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Biochemistry 2020Quote: ... 1 mM PMSF and protease inhibitor mix HP Plus (Serva)) for 15 min on ice and finally subjected to sonication using Bioruptor Plus sonication device (Diagenode). The lysed cells were centrifuged at 16,000 x g for 30 min at 4 °C and SDS sample buffer was added to the supernatant ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2020Quote: ... the pellets were resuspended in 300 μL ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON - 60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... lysed with RIPA lysis buffer with protease inhibitors on ice for 15 minutes and then sonicated with a Bioruptor (Diagenode) for 10 minutes on high with 30 seconds on and 30 seconds off ...
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bioRxiv - Cell Biology 2022Quote: ... The cell lysate was transferred to 15 mL Bioruptor Pico tubes along with ~200μL of the corresponding sonication beads (Diagenode C010200031) and sonicated using a Bioruptor Pico (Diagenode B01060010 ...
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bioRxiv - Genomics 2022Quote: ... DNase I treatment was replaced by a step of chromatin shredding by sonication in an ice-cold bath (15 min, High, 45 sec ON/OFF, Diagenode). The GelFree fractionation was omitted to avoid loss of material because the eluate after the second purification step was judged sufficiently pure.
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... The lysate was sonicated on ice at 30% amplitude for 3 minutes (15 seconds on 45 seconds off) followed by 40% amplitude for 30 seconds (15 seconds on 45 seconds off) in a Bioruptor (Diagenode). The sonicated lysate was supplemented with 1% NP-40 alternative and incubated rotating for 1 hour at 4C ...
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bioRxiv - Molecular Biology 2019Quote: ... Either the whole extract was sonicated in a 15 ml bioruptor pico tube with 300 µl of sonication beads (Diagenode) added (Figure 5B-5D ...
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bioRxiv - Molecular Biology 2019Quote: ... the WCL was fragmented as well by sonicating for 3 cycles 30 seconds on / 30 seconds off in 15 ml bioruptor pico tubes containing 300 µl of sonication beads (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2021Quote: ... vortexed briefly and sonicated for 10 cycles x (30’’ on /30’’ off, high setting condition) in 15 ml conical polystyrene tubes using a Bioruptor TM (Diagenode) sonicator with a 4°C water bath cold circulation system ...
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bioRxiv - Cell Biology 2020Quote: ... protease inhibitor cocktail), and sonicated 3x (15 sec on/30 sec off cycle, low power mode) in a Bioruptor (Diagenode). Nuclei were purified by sedimentation at 1,000 rpm for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 % Triton X-100) supplemented with 1 x HALT in a 15 ml Bioruptor tube containing one scoop of sonication beads (Diagenode) and sonicated in Bioruptor Pico (Diagenode ...
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bioRxiv - Neuroscience 2022Quote: ... cells were cross-linked with 1% paraformaldehyde for 15 minutes and Chromatins were sheared into an average 200–400 bp in length by sonication (Diagenode) and immunoprecipitated with following antibodies ...
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bioRxiv - Neuroscience 2021Quote: ... rested for 1 min on ice and sonicated (20 cycles: 15 seconds on/off; Bioruptor plus model UCD-300, Diagenode). Reduction and alkylation of disulfide bridges was performed by incubating the samples at 95 °C for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... samples from multiple ChIP experiments were pooled and sonicated for 15 cycles (30 sec ON, 30 sec OFF, high setting) with a Bioruptor (Diagenode) then concentrated with a vacuum concentrator (Eppendorf) ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate was spun at 13,000 rpm for 30 min at 4°C and the pellet was resuspended in 300 μl ChIP lysis buffer + 1mM PMSF and sonicated for 15 min (30 sec ON - 60 sec OFF) in a Bioruptor (Diagenode). The lysate was spun at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were sonicated using 40 cycles of 15s on/off at 4 °C in the Bioruptor plus (model UCD-300, Diagenode) after boiling at 99 °C for 5 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were then shaken again at RT for 5 minutes at 1000rpm before being sonicated for 15 cycles of 30 seconds on/ 30 seconds off with a BioRuptor (Diagenode). Benzonase was added to each sample and incubated at 37°C for 15 minutes to digest DNA ...
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bioRxiv - Cell Biology 2023Quote: ... the pellets were resuspended in 300 μl ChIP lysis buffer containing 1 mM PMSF and sonicated for 15 min (30s ON–60s OFF) in a Bioruptor (Diagenode). The lysates were centrifuged at 7000 rpm for 15 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Samples were then shaken again at RT for 5 minutes at 1000 rpm before being sonicated for 15 cycles of 30 seconds on/ 30 seconds off with a BioRuptor (Diagenode). Benzonase was added to each sample and incubated at 37 °C for 15 minutes to digest DNA ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were sonicated using 40 cycles of 15s on/off at 4°C in the Bioruptor plus (model UCD-300, Diagenode). Further ...
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bioRxiv - Molecular Biology 2024Quote: Multiple ChIP experiment samples were pooled and sonicated for 15 cycles (30 sec on, 30 sec off, high setting) with a Bioruptor (Diagenode), then concentrated with a vacuum (Eppendorf) ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Cell Biology 2020Quote: ... The dry samples were resuspended in 0.8 ml of 0.5% (v/v) trifluoroacetic acid (TFA) solution in HPLC-grade water with the assistance of sonication (Bioruptor Plus, Diagenode, 5 cycles of 30 s ON ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Molecular Biology 2023Quote: ... or 10 µg anti-IgG antibody (Diagenode). The next day ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by 10 min sonication (Diagenode Bioruptor). The extracts were then precleared ...
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bioRxiv - Genomics 2021Quote: ... 1 mL RIPA and 1 mL RIPA containing resuspended liver was placed into a 15 mL tube with 800 mg of Diagenode sonication beads (Diagenode, C01020031). This tube was sonicated for 30 cycles of 30s on and 30s off using a Bioruptor Pico ...
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bioRxiv - Cancer Biology 2022Quote: ... The sonication was performed in 15 cycles of 30-second on/off intervals in a Bioruptor® Pico sonication device (Diagenode). Finally ...