Labshake search
Citations for Diagenode :
1 - 50 of 402 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... 30 s ON/30 s OFF for 15 min × 3 times in Bioruptor Sonication System UCD200 (Diagenode) in 15 ml polystyrene conical tubes ...
-
bioRxiv - Neuroscience 2022Quote: ... Sonication (30 s on, 30 s off, 10 min) was performed using Bioruptor (Diagenode). Pre-cleaning of lysate was carried out using blocked Protein G sepharose (ab193259 ...
-
bioRxiv - Molecular Biology 2021Quote: ... Chromatin was fragmented by sonication (30 cycles of 15 s ‘on’ and 30 s ‘off’) with a Bioruptor (Diagenode) to 200 – 1000 bp ...
-
bioRxiv - Biochemistry 2022Quote: ... 30 s on / 30 s off (Diagenode). Lysates were cleared by centrifugation ...
-
bioRxiv - Molecular Biology 2019Quote: ... mixed by rotation at 4°C for 30 min, and then sonicated for 8 cycles (ON for 30 s, OFF for 30 s) using a Picoruptor Sonicator (Diagenode). Each tube of lysate was incubated overnight at 37°C in hybridization buffer (750 mM NaCl ...
-
bioRxiv - Cell Biology 2022Quote: ... The lysates were sonicated (30 s/30 s, 10 cycles, high performance) by Bioruptor (Diagenode; B01020001), followed by centrifugation at 21000xg for 20 min at 20°C ...
-
bioRxiv - Genomics 2021Quote: ... 0.3% NP-40, 10% glycerol, 1XPIC) and sonicated for 10 cycles (30 s on, 30 s off) in a Bioruptor (Diagenode). SDS loading dye was added and the samples were boiled before immunoblotting.
-
bioRxiv - Biochemistry 2022Quote: ... Crosslinked proteins were denatured in 8 M urea 50 mM Tris and sonicated for 15 cycles of 30 s with a Bioruptor Plus (Diagenode, SA) at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... 30 s on/30 s off at 4° C (Bioruptor, Diagenode). One tenth of the sample was taken as input control ...
-
bioRxiv - Plant Biology 2020Quote: ... Nuclei were disrupted by sonication 10 times for 30 s each and 30 s of cooling in a Bioruptor (Diagenode). IPs were performed using α-GFP (Ab6556 ...
-
bioRxiv - Molecular Biology 2021Quote: ... The supernatant was kept on ice and the pellet was resuspended in 200 µL OLB with 500 mM NaCl and sonicated for 10 min at high setting with 30 s / 30 s duty cycle on a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 20,000 × g at 4 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... DNA was sheared by sonication for 10 min at high settings (30 s on, 30 s off) using a Bioruptor Pico sonicator (Diagenode) to reduce sample viscosity ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... and sonicated for 10 min (medium intensity, 30 s on/30 s off) in a Bioruptor UCD-200 system (Diagenode), and centrifuged at 21,000 x g for 5 min ...
-
bioRxiv - Cell Biology 2020Quote: ... 4 × 5 min cycle (30 s on/ 30 s off; Diagenode Bioruptor) with 4 min incubation on ice between sonication cycles ...
-
bioRxiv - Molecular Biology 2023Quote: ... the nuclear pellet was resuspended in 300 µl IP buffer and sonicated four times for 10 minutes (30 s in “on” mode, 30 s in “off” mode) using the Bioruptor Plus (Diagenode, Belgium). Sheared chromatin was collected by centrifugation and a 10-µg sample was used for IP ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were sonicated using a Bioruptor Plus from Diagenode (15 cycles of 30 s). The protein concentration was measured using a tryptophan assay ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were disrupted by sonication for 10 min (alternating 20 s off and 40 s off) using a Bioruptor Plus (Diagenode, Seraing, Belgium). Cell debris was removed by centrifugation at 14,000 rpm for 1 h at 4 °C and the supernatant was retained ...
-
bioRxiv - Neuroscience 2023Quote: ... the proteins were extracted by sonication executing 40 cycles of 15s on and 15 s off at 4 °C using a Bioruptor plus (model UCD-300, Diagenode). After lysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
-
bioRxiv - Cell Biology 2021Quote: ... Lysates were sonicated (5× cycles of 30 s on and 30 s off; Bioruptor, Diagenode) and clarified by centrifugation (16,000 × g for 15 min at 4°C ...
-
bioRxiv - Cell Biology 2020Quote: ... sonicated (5 cycles of 30 s ON, 30 s OFF, high power, Bioruptor Plus, Diagenode), spun for 10 min at 16,000 × gmax to remove any insoluble material ...
-
bioRxiv - Cancer Biology 2023Quote: ... 30 s on / 30 s off) at 4°C using a water bath-sonicator (Diagenode). Chromatin fragments were reverse cross-linked overnight at 65°C ...
-
bioRxiv - Molecular Biology 2019Quote: ... and sonicated for 38 cycles of 30 s ON and 30 s OFF (Bioruptor sonicator, Diagenode). Cleared chromatin was incubated overnight with 10 μg α-KLF4 (sc-20691) ...
-
bioRxiv - Microbiology 2021Quote: ... and sonicated for 45 min (intervals: 20 s on, 40 s off) using a Bioruptor (Diagenode). After clearing the lysates ...
-
bioRxiv - Neuroscience 2022Quote: Sonication (30 s on, 30 s off, 30 min)59 was performed using a bioruptor (Diagenode) and the fragment size 200–500 bp was verified ...
-
bioRxiv - Cell Biology 2023Quote: ... and subjected to sonication (30 s on and 30 s off, 80 times, Bioruptor 300 [Diagenode]). The supernatant was collected by centrifugation ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
-
bioRxiv - Neuroscience 2024Quote: ... and sonicated with 30 cycles of 15 s ON-OFF using a BioRuptor (Diagenode, Denville, NJ-USA). The EV lysate was reduced with 10 mmol/L dithiothreitol at 56 °C for 30 minutes ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysates were sonicated 18 cycles of 30 s on and 30 s off (Bioruptor Pico, Diagenode) and insoluble material was discarded by centrifugation at 13000rpm for 20 minutes at 4°C ...
-
bioRxiv - Genomics 2019Quote: ... Samples were sonicated for 20 cycles (30 s ON, 30 s OFF) using a Bioruptor Pico sonicator (Diagenode) in 15 ml Diagenode TPX tubes containing 0.3 ml polypropylene beads ...
-
bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
-
bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
-
bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were sonicated for 7.5 min (30 s on, 30 s off cycles) using a Bioruptor waterbath sonicator (Diagenode), then cleared by centrifugation at 16,000 g for 15 min at 4 °C ...
-
bioRxiv - Systems Biology 2023Quote: ... By ultrasonically treating the pellet three times for 30 s with intervals of 30 s with a Bioruptor (Diagenode), the proteins precipitated with acetone for 3 h at −30 °C were dispersed in 50 mM triethylammonium bicarbonate ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
-
bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
-
bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
-
bioRxiv - Genomics 2021Quote: ... Pellet was then resuspended in lysis buffer for 5 min on ice and sonicated as follows: 16 cycles of 30 s on / 30 s off at max power (Bioruptor Diagenode), and centrifuged at 14,000 rpm at 4 °C for 10 min ...
-
bioRxiv - Molecular Biology 2021Quote: ... by sonication for four cycles of 30 s pulses with low power and 60 s cooling between pulses using a Bioruptor UCD-200 (Diagenode). Following centrifugation (16,100g for 10 min at 4°C) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
-
bioRxiv - Genomics 2022Quote: ... by boiling at 95°C for 5 minutes followed by 5 sonication cycles of 30 s ON / 30 s OFF on the Bioruptor Pico sonicator (Diagenode) at 4°C ...
-
bioRxiv - Microbiology 2022Quote: ... and incubation on ice for 30 min followed by sonication for 45 min (30 s on, 30 s off) in a Bioruptor Pico (Diagenode) at 4°C ...
-
bioRxiv - Plant Biology 2022Quote: ... Samples were sonicated for 4 min on a 30 s on/30 s off cycle using a Bioruptor Pico (Diagenode). Subsequent steps were performed as in the Universal Plant ChIP-seq kit protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... the resuspended solution was subjected to sonication for 22 cycles (30 s on/30 s off per cycle) with Bioruptor Plus (Diagenode). The lysate was centrifuged with 8,000g at 4°C for 15 minutes ...
-
bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
-
bioRxiv - Developmental Biology 2023Quote: ... The samples were sonicated five times (30-s pulses with 30-s break interval) using the Bioruptor water bath sonicator (Diagenode). The chromatin extracts were then centrifuged at 9,400 g for 10 min at 4 °C ...