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Citations for Diagenode :
301 - 333 of 333 citations for DNA Polymerase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cross-linked chromatin was isolated and sonicated to generate DNA fragments averaging 200-500 bp in length by Bioruptor plus sonication device (Diagenode). Chromatin fragments were immunoprecipitated with antibodies directed against ZEB1 (1 μg ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatin DNA was sonication for 30 min on ice (30 s with 30 s intervals) with the Bioruptor Plus device (Diagenode) to shear the chromatin to an average size of 200 bp to 500 bp ...
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bioRxiv - Microbiology 2023Quote: ... and chromosomal DNA was sheared to 100-500 bp fragments using the Bioruptor Pluswater bath sonication system (Diagenode, Liège, Belgium). Lysates were incubated with 40 μL Anti-FLAG M2 affinity gel (cat A2220 ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Microbiology 2023Quote: ... was subjected to a brief sonication on ice for shearing the genomic DNA using Diagenode Bioruptor Plus sonicator (Diagenode Inc.). The supernatant was further centrifuged at 10000 x g for 5 minutes ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then pooled sixteen by sixteen and DNA of each pool was sheared to a mean size of ca 400 bp using the Bioruptor® Pico (Diagenode) (15sec ON / 90sec OFF for 8 cycles) ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin immunoprecipitation was then performed on the sheared DNA using the iDeal ChIP-seq kit for transcription factors (Diagenode Cat# C01010170). One percent of chromatin was kept aside to be used as an input control ...
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bioRxiv - Plant Biology 2021Quote: ... The IP of the methylated DNA was performed overnight at 4°C using Protein A Dynabeads coated with anti-5mC (Diagenode, C15200081) or anti-IgG (Diagenode ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Molecular Biology 2019Quote: ... DNA was fragmented by vortex and further sheared to DNA fragment sizes ranging between 200-500 bp with a Bioruptor® waterbath sonicator (Diagenode) at 4 °C for 40 cycles of 30 seconds ON/OFF at the middle power setting ...
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bioRxiv - Plant Biology 2022Quote: ... and then the mixture was sonicated to shear DNA into 250 to 600 bp fragments using Bioruptor Plus sonication system (Diagenode, Belgium). The lysates were centrifuged at 20,000 × g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Genomics 2023Quote: ... ≥2 ug of high molecular weight genomic DNA (more than 50% of fragments ≥40 kb) was sheared to ∼20 kb using the Megaruptor (Diagenode B06010002), followed by DNA repair and ligation of ONT adapters using the ONT Ligation Sequencing Kit (SQK-LSK109) ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genomics 2022Quote: ... RRBS libraries were prepared for 23 samples with 200 ng genomic DNA according to the protocol described previously (Diagenode Premium RRBS kit) and kept at -80°C until submission to the GGBC for sequencing ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 200ng of DNA from each sample (male pool and female pool) was briefly sonicated using a Bioruptor sonication device (Diagenode, Liege, Belgium), and then end-repaired and size-selected on beads to retain fragments of size around 550 bp ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: A total of 100 ng of total genomic liver DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... and we fragmented genomic DNA (normalized to 23 ng/µL) to 400-600 using the Bioruptor UCD-300 sonication device (Diagenode, Denville, NJ, USA).
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A total of 100ng of total DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...
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bioRxiv - Microbiology 2020Quote: ... gDNA was subjected to agarose gel electrophoresis and OD ratio tests to confirm the purity and concentration of the DNA prior to Bioruptor (Diagenode, Inc., Denville, NJ USA) fragmentation ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Microbiology 2023Quote: ... Two µg of high molecular weight genomic DNA (final volume of 100 µL at 20 ng/µL) were sheared using the Megaruptor3 (Diagenode inc, Denville, New Jersey, USA) at speed The DNA Damage repair ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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bioRxiv - Cancer Biology 2020Quote: ... Purified DNA was fragmented to obtain fragments of an average size of 300–400 bp using a Bioruptor Pico (Diagenode; 8 cycles; 20 sec ON/60 sec OFF). 3 μg of DNA per condition were used for library preparation ...