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Citations for Diagenode :
251 - 295 of 295 citations for 7 Chloro 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Microbiology 2021Quote: ... boiled at 95°C for 10 min and sonicated for 5 min using a Bioruptor® Plus sonication device (Diagenode, Liège, Belgium). Protein lysates were then run on a precast NuPAGE Bis-Tris 4–12% SDS–PAGE (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and the samples were heated for 5 min at 95°C prior to sonication with a Bioruptor UCD-200 (Diagenode, Seraing, Belgium) device for ten cycles of 1 min pulse and 30 sec pause at high power ...
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bioRxiv - Plant Biology 2023Quote: ... Slides were blocked with 4% BSA for 30 minutes at 37°C in a moist box followed by primary (anti-H3K9m2, C15410060, Diagenode, 1:500) and secondary (Alexa Fluor 488 Goat anti-Rabbit IgG1 Secondary Antibody ...
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bioRxiv - Plant Biology 2019Quote: ... and sonicated 2 cycle x 30 second ON/ 30 second OFF using Bioruptor® Plus (Diagenode). After removal of cell debris by centrifugation (5 min ...
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bioRxiv - Microbiology 2023Quote: ... About 2 µg of genomic DNA was sheared by sonication with a Picoruptor (Diagenode, S.A., Belgium) instrument to a size of 300–400 bp in length ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Neuroscience 2019Quote: ... Fig.2A) using a closed system ultrasonic disruptor (10 cycles of 30s ON/30s OFF, 4°C; Bioruptor Pico, Diagenode sa, Liege, Belgium). ChIP assays were then conducted using antisera to CTCF (61311 ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Developmental Biology 2019Quote: ... the amplified cDNA (2 μg per sample) was fragmented to 200 bp using a Bioruptor Sonicator (Diagenode), and barcode ligation and end repair were performed using the Ovation Rapid DR Library System (NuGEN) ...
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bioRxiv - Genetics 2020Quote: ... The DNA was sonicated for 2 cycles of 15sec ON/90 sec OFF in a bioruptor (Diagenode) at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... and the nuclei were resuspended in 50 µL transposition mixture (25 µL 2× Tagmentation buffer (Diagenode, C01019043), 2.5 µL Tagmentase loaded (Diagnode ...
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bioRxiv - Microbiology 2019Quote: ... incubated at 95°C for 10 min and further sonicated for 15 cycles à 30 s at high intensity level and 4°C using Bioruptor®Plus (Diagenode, Liege, Belgium). Protein concentrations were determined by measuring the tryptophan fluorescence at an emission wavelength of 350 nm using 295 nm for excitation with an Infinite® M1000 PRO microplate reader (Tecan ...
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bioRxiv - Cancer Biology 2023Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Systems Biology 2020Quote: ... Chromatin was broken into small pieces with an average size of 0.5-2 kb using the Bioruptor (Diagenode). The sonicated chromatin was then incubated with 5 µg of primary antibodies overnight at 4°C ...
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bioRxiv - Evolutionary Biology 2019Quote: HMW genomic DNA was sheared to 30 kb using a Megaruptor® 2 instrument (Diagenode SA, Seraing, Belgium). DNA-damage and end repair was carried out prior to blunt adaptor ligation and exonuclease purification using ExoIII and ExoVII ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Microbiology 2022Quote: ... The DNA was sheared to a modal size of 10 kb using a Megaruptor 2 (Diagenode Diagnostics, Seraing (Ougrée), Belgium) ...
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bioRxiv - Molecular Biology 2023Quote: ... All samples were lysed the same day 2 weeks before proteomics analysis in 100µL iST-NHS-Lysis buffer (PreOmics GmbH) by sonication (Bioruptor (DIagenode) 15s ON/15s OFF for 15 min) ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Immunology 2021Quote: ... Samples were split into 2 x 1.5 mL in 15 mL polypropylene tubes suitable for the Bioruptor® Pico (Diagenode). BioRuptor Sonicator settings ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were lysed and sonicated in the presence of 0.2% SDS for 30 cycles (30 s on, 30 s off) using the high setting on a Bioruptor sonicator (Diagenode). Cell debris were pelleted by centrifugation and SDS in the lysate supernatants was diluted to 0.07% ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Sonication was performed by splitting each sample to 2 Bioruptor tubes with sonication beads and using the Bioruptor Pico (Diagenode) for 18 cycles of 30 sec on and 30 sec off into an average size of approximately 200 bp ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was transferred to a sterile 2 ml microcentrifuge tube and sonicated with the Bioruptor UCD-200 sonicator (Diagenode) for 1 min on the low setting (160W at 20kHz ...
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bioRxiv - Neuroscience 2020Quote: ... the crosslinked cells were resuspended in 2 mL of lysis buffer and sonicated (in a cold room) by using a Bioruptor (Diagenode) with the following parameters ...
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bioRxiv - Genomics 2020Quote: ... high molecular weight DNA was diluted to a concentration of 25 ng/uL and fragmented to an average peak size of 25 kb using a Megaruptor-2 instrument (Diagenode). Small fragments <1 kb were removed and the DNA concentrated using bead purification (0.4 x volumes of Ampure-XP beads ...
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bioRxiv - Immunology 2021Quote: ... 2 µg of antibody was used per reaction. H3K9me3 (Lot. A2217P) and H3K4me3 (Lot No. A1052D) antibodies were from Diagenode. Immunoprecipitated and input DNA were extracted by phenol-chloroform extraction and used for qPCR with SsoAdvanced Universal SYBR Green supermix (Biorad) ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were lysed on ice for 10 minutes prior to sonication for either 20 cycles (replicate 1) or 40 cycles (replicate 2) of 30s on / 30s off with a Bioruptor UCD-200 (Diagenode) at maximum power ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... 600 ng of denatured DNA was incubated with 2 mg of 5-methylcytosine (5-mC) monoclonal antibody ([33D3] Diagenode, Cat No # C15200081) in IP buffer (0.1% SDS ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Genetics 2021Quote: ChlPseq was performed on 20k FACS sorted MM cells as previously described 44 using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) but with minor modifications on the preparation of input controls ...
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bioRxiv - Genomics 2022Quote: ... and 12 μg of high molecular weight gDNA was sheared to a size distribution between 15 and 20 kbp using the Megaruptor® 2 (Diagenode, Denville, NJ, USA). For each accession ...