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Citations for Diagenode :
151 - 200 of 295 citations for 7 Chloro 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... The mixture was heated to 95°C for 10 min and sonicated in a Bioruptor (Diagenode, Liege, Belgium) (30 sec sonication ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Developmental Biology 2024Quote: ... The sonication of samples was then performed at 4°C using a Bioruptor® Plus sonication device (Diagenode) at high-intensity settings ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Physiology 2023Quote: ... and 500-µl volume) in a water bath sonicator set at 4°C (Bioruptor 300. Diagenode, Denville, NJ). After centrifuging at 10,000g for 10 mins at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Molecular Biology 2019Quote: The soluble fraction was isolated by centrifugation at 2,000 rpm for 3 min then sonicated using a bioruptor (Diagenode) for 30 min in bursts of 30 s on/30 s off at high level in a 4°C water bath to produce sheared chromatin with a size range of 200-1,000 bp ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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bioRxiv - Genetics 2023Quote: ... 3) around 400 μl of the slurry were transferred to 0.6 ml tubes and sonicated in the Bioruptor (Diagenode) at + 4°C for 1 hour on the “high” setting with sonication intervals set to 45 seconds on/15 seconds off ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2021Quote: Genomic DNA was sheared to 20 kb fragments using the Megaruptor 2 (Diagenode) and size selected with a 10 kb cut-off using the BluePippin system (Sage Science) ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... Genomic DNA was sheared to 20-kb fragments using Megaruptor 2 (Diagenode, Belgium) and then bead-size selected with AMPure PB beads (Pacific Biosciences ...
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bioRxiv - Zoology 2022Quote: ... 2 μg genomic DNA was sonicated using a Picoruptor (Diagenode s.a., Liège, Belgium) to produce 300–500-bp fragments ...
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bioRxiv - Plant Biology 2022Quote: HMW DNA was sheared using Megaruptor 2 system (Diagenode, Liège Science Park, Belgium) to obtain a 40 Kb average size ...
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bioRxiv - Cell Biology 2023Quote: ... 2 μl of precleared chromatin was added to 98 μl buffer iE1 (Diagenode). To samples and inputs ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... SDS lysates were boiled at 95°C for 10 minutes and then 10 cycles in a Bioruptor Pico (Diagenode) of 30 seconds on and 30 second off per cycle ...
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Deep learning the collisional cross sections of the peptide universe from a million training samplesbioRxiv - Systems Biology 2020Quote: ... The samples were boiled at 95°C for 10 min and subsequently sonicated at maximum power (Bioruptor, Diagenode, Belgium). Proteolytic digestion was performed overnight at 37 °C by adding either i ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were sonicated for 5 cycles of 30 s on/off (2.5 min total sonication time) at 4°C in a Bioruptor Plus sonication system (Diagenode) and pelleted by centrifugation at 8000 RPM for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... in the presence of protease inhibitors and subjected to 3 × 10 cycles (30 seconds on and 30 seconds off) of sonication (Bioruptor, Diagenode) to generate chromatin fragments of ∼300 bp in length ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Genetics 2019Quote: ... 2 μl of IP material were resuspended in 30 ul ChIP-seq buffer (Diagenode) supplemented with 1 μl Tn5 (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: ... Genomic DNA was sheared at 20kb target size with a Megaruptor 2 instrument (Diagenode). BluePippin size selection (Sage Science ...
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bioRxiv - Systems Biology 2023Quote: ... and 2 mM EDTA at pH 8.0) with H3K4me3 (0.005μg/μl, C15410003-50, Diagenode), H3K9me3 (0.005μg/μl ...
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bioRxiv - Genomics 2023Quote: ... 2 µg of genomic DNA were sheared on a Megaruptor3 instrument (Diagenode, Seraing, Belgium) to a fragment size of about 18 kb ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...