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Citations for Diagenode :
2401 - 2450 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... followed by sonication at high power for 10 cycles of 30s ON/OFF (Bioruptor Plus; Diagenode). Lysates were clarified by centrifugation at 10,000g for 15min ...
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bioRxiv - Genomics 2020Quote: Total genomic DNA was sheared by sonication for 15–24 min using a Bioruptor (Diagenode, Inc., New Jersey, USA). Size-selected samples (200–400 bp ...
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bioRxiv - Genomics 2020Quote: ... 73 µl of 1X buffer iC1 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) supplemented with Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was fragmented to 20 kb using Megaruptor 2 (Diagenode, Liege, Belgium) and size selected with a 10 kb cut-off using the Blue Pippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was sheared to 20 kb fragments using Megaruptor 2 (Diagenode) and size selected using the BluePippin system (Sage Science ...
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bioRxiv - Genomics 2020Quote: ... genomic DNA was sheared using the Megaruptor 2 (Diagenode) with a long hydropore and a 20 kb shearing protocol ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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bioRxiv - Genomics 2020Quote: DNA obtained from agarose plugs was sheared down to ~ 40 kbp fragment size with a MegaRuptor™ device (Diagenode, Belgium), fragmented using Covaris g-tubes (#520079 ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 × 5 min (30 sec on ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were lysed for 15 min on ice with occasional inversion every 2 min and then sonicated for 24 cycles with 30s on and 30s off using Bioruptor (Diagenode). After sonication ...
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bioRxiv - Genomics 2020Quote: ... The extract was sonicated to an average size of ∼250 base pairs using a Bioruptor Pico (Diagenode), and 10-20 ug of DNA was used per ChIP reaction ...
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bioRxiv - Genomics 2020Quote: ... and then fragmented by sonication (either Q800R2 Sonicator (Qsonica) or Bioruptor Plus (Diagenode)) to 800-1000bp fragments ...
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bioRxiv - Genomics 2020Quote: ... Chromatin was sheared 10 × 30 sec on/30 sec off with a Bioruptor Pico (Diagenode). Supernatant with chromatin extract was isolated after a 10 minutes centrifugation at 14.000g at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Nuclei were extracted according to 81 and sonicated for 16 cycles (30s ON, 30s OFF, on high setting) in a Bioruptor Plus (Diagenode) to achieve a DNA fragment size below 500 bp ...
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bioRxiv - Genomics 2020Quote: ... ChIP was performed using True MicroChIP Kit (Diagenode #C01010130) according to the manufacturer’s instructions with the following modifications ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 x 5 min (30 sec on ...
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bioRxiv - Genomics 2020Quote: ... We sheared each BAC DNA (5 µg) separately using a Biorupter Pico (Diagenode) to 100-500 bp size ...
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bioRxiv - Genomics 2020Quote: ... and chromatin was sheared by sonication using a Picoruptor® (Diagenode) for 10 cycles ...
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bioRxiv - Genomics 2020Quote: ... Extracted DNA was either left unfragmented or sheared using either MegaRuptor (Diagenode) or by passing DNA through a 100µl pipette tip 10-20 times ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Genomics 2020Quote: ... chromatin was sonicated with a Biorupter (Diagenode) to generate 300-600 bp chromatin fragments and immunoprecipitated with antibody plus Protein A:G beads ...
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bioRxiv - Bioengineering 2020Quote: ... for 1 h at room temperature. The anti-AAV2 (A20, cat. # 03-65155, American Research Products, Inc) and anti-SpCas9 antibody (Diagenode C15310258) was used as positive control for detection of anti-AAV9 and anti-SpCas9 antibodies ...
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bioRxiv - Bioengineering 2020Quote: ... rabbit polyclonal anti-Cas9 (C15310258-100, 1:1000, Diagenode, Denville, NJ) and rabbit monoclonal anti-Gapdh (#2118 ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was sheared to an average size of ∼200-300 bp using Biorupter (Diagenode). 10% of sonicated fraction was processed as input using phenol–chloroform and ethanol precipitation ...
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bioRxiv - Genetics 2020Quote: ... Chromatin was sheared to an average size of 150 bp using a sonicator (Bioruptor Pico, Diagenode). Sonicated chromatin fragments were immunoprecipitated with 3 μg of anti-Rad21 (Abcam ...
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bioRxiv - Genomics 2020Quote: ... The chromatin was sheared to fragment size in the range of 200–500 bp using a Bioruptor (Diagenode). The solubilized chromatin fraction was cleared by centrifugation and used for immunoprecipitation after diluting 5 times with sonication buffer ...
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bioRxiv - Genomics 2020Quote: ... at 1g of chromatin per 3.2mL of buffer and sonicated on a Bioruptor NextGen sonication device (Diagenode) to shear the chromatin to an average fragment size of 200 - 500 bp as determined by agarose gel electrophoresis ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Genetics 2020Quote: ... Chromatin was sonicated using a Bioruptor Pico (Diagenode), to an average size of 200-700 bp ...
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bioRxiv - Genetics 2020Quote: ... The purified gDNA was resuspended in 100 μl TE and sonicated using a BioRuptor Plus (Diagenode) with settings ...
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bioRxiv - Genomics 2020Quote: ... chromatin was sheared to 200-500 bp fragments on a Bioruptor Plus (Diagenode; 2x 9 cycles of 30 sec on and 30 sec off at the highest power setting) ...
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bioRxiv - Genomics 2020Quote: ... rabbit polyclonal anti-H3K27me3 (1:1000; Diagenode C15410069); mouse monoclonal anti-p21 (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies (AR, 9 μg, Abcam, ab74272; H3K27ac, 1 μg, Diagenode, C15410196) were incubated with 40 μL of protein A/G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genetics 2020Quote: ... 2 cycles of sonication (30s ON/30s OFF) were performed using a Bioruptor Pico (Diagenode) to remove contaminants while minimising chromatin shearing74 ...
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bioRxiv - Developmental Biology 2020Quote: ... The worm suspension containing fixed DNA-protein complexes was sheared using a Bioruptor (Diagenode, Denville, NJ) on the high setting for 20 cycles (30 seconds ON/30 seconds OFF) ...
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bioRxiv - Molecular Biology 2020Quote: ... and immediately sonicated on the Bioruptor (Diagenode) in 1.5 mL Eppendorf tubes at high power for three 10-minute cycles of 30 seconds on and 30 seconds off ...
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bioRxiv - Cancer Biology 2020Quote: ... Sonication of crosslinked chromatin was performed using a Bioruptor® UCD-200™Sonicator (Diagenode) with optimized cycles for each cell type ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was sheared into 200-400 bp fragments using a Bioruptor sonicator (Diagenode), clarified by centrifugation at max speed for 10 minutes ...
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bioRxiv - Immunology 2020Quote: ... Cellular lysates were sonicated for 40 minutes (30sec ON, 30sec OFF) on high in a Bioruptor UCD-200 (Diagenode). After sonication ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated using a Bioruptor bath sonicator (Diagenode), centrifuged at 10,000 rpm for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated using a Bioruptor bath sonicator (Diagenode), and centrifuged at 10,000 rpm for 10 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... sonicated with Biorupter® Sonicator for 10 cycles (Cat#B01020001, Diagenode), and centrifuged at 13,000 rpm for 10 min ...
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bioRxiv - Genomics 2020Quote: ... C15410193 (Diagenode 1μg/ChIP); H3K27me3 ...
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bioRxiv - Genomics 2020Quote: ... C15410196 (Diagenode 1μg /ChIP); Med1 ...
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bioRxiv - Genetics 2020Quote: ... Chromatin shearing was performed on a Bioruptor Pico (Diagenode) for 10 min (30 seconds on / 30 seconds off ...
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bioRxiv - Genetics 2020Quote: ... sheared on a Bioruptor Pico (Diagenode) for 10 min (30 s on/off cycling) ...