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Citations for Diagenode :
2151 - 2200 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Tris-HCl pH8.1 50mM) and transferred to 1.5mL Bioruptor+ TPX microtubes (#C30010010-300, Diagenode, Seraing, Belgium). Samples were sonicated on the Bioruptor Plus next-gen ultrasonicator (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin lysate was sonicated to an average size of ~250 bp using 40 cycles of sonication (30 sec on/off High-Power setting; Diagenode Biorupter Plus). A 20% fraction of the sonicated chromatin was incubated with one of the following antibodies ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Microbiology 2021Quote: ... or 30 kb using using Covaris LE220 or g-Tubes or Megaruptor3 (Diagenode). The sheared DNA was treated with exonuclease to remove single-stranded ends and DNA damage repair mix followed by end repair and ligation of blunt adapters using SMRTbell Template Prep Kit 1.0 (Pacific Biosciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Lysates were sonicated on a Bioruptor (Diagenode), and diluted 10x in Immunoprecipitation Dilution Buffer (Tris-HCl pH 8.1 20mM ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were then sonicated in a Bioruptor (Diagenode) for 30s on/ 30s off (x5) ...
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bioRxiv - Synthetic Biology 2021Quote: ... with 437.5 mg sonication beads (Diagenode, C03070001) for 6 cycles on a Bioruptor Pico sonicator ...
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bioRxiv - Cell Biology 2021Quote: ... sonicated three times at maximum intensity (Bioruptor®, Diagenode, Belgium) and the samples were denaturated at 65 °C for 15 min ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were sonicated using a Bioruptor (Diagenode) to achieve a chromatin size distribution of 200-1000 bp ...
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bioRxiv - Genomics 2021Quote: ... Sonication was performed with a Bioruptor Standard Sonicator (Diagenode) and consisted of two rounds of 15 min each ...
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bioRxiv - Genomics 2021Quote: ... Sonication was performed on a Bioruptor Plus (Diagenode, Seraing, Belgium).
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bioRxiv - Cancer Biology 2021Quote: H3K27ac chromatin immunoprecipitation (ChIP) experiments were performed as previously described12 using the iDeal ChIP-seq kit for histones (Cat# C01010171, Diagenode) and the H3K27ac rabbit polyclonal antibody (Abcam Cat# ab4729 ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... DNA libraries for all 12 samples were prepared using the MicroPlex Library Preparation Kit (12 indexes; Diagenode) following the manufacturer’s recommendations ...
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bioRxiv - Immunology 2021Quote: ... Chromatin immunoprecipitation was performed using the iDeal ChIP-seq kit for Transcription Factors (Diagenode) as per the manufacturer’s recommendations ...
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bioRxiv - Genomics 2021Quote: ... 500 ng total gDNA was sheared to 100-1000bp using Bioruptor Pico (Diagenode), then purified with 1.2X volume of SPRI beads (Beckman Coulter ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (Diagenode) following the manufacturer’s protocol with some modifications ...
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bioRxiv - Genomics 2021Quote: ... H3K4me1 (pAb-037-050) and H3K79me2 (pAb-051-050) from Diagenode (Denville NJ), CTCF (Cat #2899 ...
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bioRxiv - Genomics 2021Quote: ... H3K27me3 (1:100, C15410069, Diagenode), and SOX9 (1:100 ...
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bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
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bioRxiv - Genomics 2021Quote: ... The cells were lysed by high energy sonication (Bioruptor, Diagenode) and reduced by adding DTT (to a final concentration of 15 mM and heating for 30 minutes at 56 °C) ...
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bioRxiv - Genomics 2021Quote: ... samples were sonicated (Bioruptor, Diagenode, Belgium) with the following settings ...
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bioRxiv - Genomics 2021Quote: ... samples with different P1 barcodes were pooled together and sheared via sonication with a Bioruptor Pico (Diagenode) using three cycles of 45 s ON and 60 s OFF at 4°C ...
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bioRxiv - Genomics 2021Quote: ... Samples were sonicated to an average size of 200 bp using a Bioruptor Pico (Diagenode) and NEBNext Multiplex reagents and sequencing adapters were used to prepare sequencing libraries following the Illumina NEBNext DNA library prep kit instructions (New England Biolabs) ...
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bioRxiv - Genomics 2021Quote: ... Genomic DNA or DNA previously fragmented to 50 kb with a Megaruptor (Diagenode S.A., Liege, Belgium) was first size-selected using a BluePippin (Sage Science ...
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bioRxiv - Genomics 2021Quote: The protocol for Premium RRBS kit (Diagenode) library preparation included enzymatic digestion of 100 ng of gDNA with MspI ...
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bioRxiv - Genomics 2021Quote: ChIP antibodies were purchased from Diagenode: H3K4me3 (#C15410003) ...
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bioRxiv - Genomics 2021Quote: ... cells were lysed by SDS Lysis Buffer and sonicated for 1.5 hrs (Diagenode Bioruptor) at 4°C on the high setting with 30 seconds on/off interval ...
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bioRxiv - Genomics 2021Quote: ... Mouse anti-Cas9 (Encor MCA-3F9 and Diagenode C15200229-100) antibodies were used ...
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bioRxiv - Genomics 2021Quote: ... ChIP was performed using Cas9 (Diagenode C15200229-100), Flag-M2 (Sigma ...
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bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Genomics 2021Quote: ... 1 mL RIPA and 1 mL RIPA containing resuspended liver was placed into a 15 mL tube with 800 mg of Diagenode sonication beads (Diagenode, C01020031). This tube was sonicated for 30 cycles of 30s on and 30s off using a Bioruptor Pico ...
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bioRxiv - Genomics 2021Quote: ... and H3K27me3 (C15410196, C15410193, and C15410195, Diagenode, respectively), FOXA1 (ab23738 ...
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bioRxiv - Genomics 2021Quote: ... Lysates were sonicated for 15 cycles (30 sec on / 30 sec off) at 4°C using a Bioruptor Pico sonicator (Diagenode). After sonication ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: Illumina libraries were constructed with primer indices provided by the Auto-Chipmentation kit for histones (Diagenode,cat. C01011000), according to the protocols supplied ...
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bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
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bioRxiv - Genomics 2021Quote: ... ChIP was performed with the Auto-Chipmentation Kit for histones (Diagenode, cat. C01011000). Crosslinked chromatin was resuspended in 100 µL cold lysis buffer IL1 and incubated at 4°C for 10 min in a rotating well ...
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bioRxiv - Genomics 2021Quote: The same ChIP protocol was performed with the Auto-Chipmentation kit for histones (Diagenode,cat. C01011000), with specific antibodies validated for M ...
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bioRxiv - Genetics 2021Quote: ... lysed at high performance with a Bioruptor Plus sonicator (Diagenode) using 10 cycles of 30 s sonication intervals ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Evolutionary Biology 2021Quote: ... A DNA sonication step was included (Diagenode Bioruptor) followed by digestion for 1.5 hrs ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were sonicated with the Bioruptor Pico (Diagenode) machine (10 cycles ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM PMSF, pH 8.5) and sonicated for 5 min (30 sec on, 1 min off) with Bioruptor UCD-200TM (Diagenode, Liège, Belgium). The resulting cell lysates were centrifuged ...
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bioRxiv - Genomics 2021Quote: ... Pellet was then resuspended in lysis buffer for 5 min on ice and sonicated as follows: 16 cycles of 30 s on / 30 s off at max power (Bioruptor Diagenode), and centrifuged at 14,000 rpm at 4 °C for 10 min ...
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bioRxiv - Genomics 2021Quote: ... Global DNA methylation profiling was generated with the Infinium MethylationEPIC BeadChip Kit (Queensland University of Technology and Diagenode, Austria). The first batch contained only males and were randomized for timepoint and age ...
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bioRxiv - Genomics 2021Quote: ... DNA (400 ng) was fragmented to approximately 300—700 bp using a BioRuptor (Diagenode) using 2 or 4 cycles of 15 sec ON / 90 sec OFF at instrument setting HIGH ...
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bioRxiv - Genomics 2021Quote: ... samples were loaded into a Bioruptor (Diagenode, Denville, NJ) and sonicated with repeated cycles (10 minutes per cycle ...
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bioRxiv - Physiology 2021Quote: ... Probes were then homogenized at 4°C in Bioruptor Pico sonicator (Diagenode), using 30 sonication cycles (30 s on/off) ...
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bioRxiv - Biochemistry 2021Quote: ... MNase-digested nuclei slurry was sonicated using Bioruptor 300 (Diagenode) to disrupt the nuclear membrane ...