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Citations for Diagenode :
151 - 200 of 342 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... at 4°C for 13 cycles with 30 sec on and 30 sec rest in 15 ml Bioruptor Tubes (Diagenode Cat #C30010017) with 650 mg of beads (Diagenode Cat #C03070001) ...
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bioRxiv - Neuroscience 2024Quote: ... and sonicated for 15 min (cycles of 30 sec ON and 30 sec OFF, 4°C, low frequency) in a Bioruptor Pico (Diagenode, Seraing, Belgium). Samples were subsequently incubated for 15 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... by sonication for 15 min (30 s on/off cycles) at 4 °C with high power in a Bioruptor device (Diagenode, Liège, Belgium). Subsequently ...
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bioRxiv - Immunology 2024Quote: ... We transferred cells into 1.5 ml sonication tubes (200 µL/tube) and sonicated 30 sec on/30 sec off for 6 cycles at 4 °C using Bioruptor® Pico (Diagenode, Denville, NJ). We centrifuged fragmented chromatin at 20,800 g for 10 min at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting chromatin was incubated overnight at 4°C with histone modification antibodies (H3K27ac, Abcam ab4729; H3K27me3, Diagenode C15410195 and H3, Abcam ab1791) pre-conjugated with Dynabeads Protein G ...
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bioRxiv - Developmental Biology 2024Quote: ... 1% PIC) and sheared to 100-600bp fragments (average 250bp) at 4°C in a Bioruptor® Pico sonication device (Diagenode: Denville, NJ) for 5 cycles (30 sec on ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was sheared by sonication using a Bioruptor (Diagenode, 9 cycles of 30 s on/off). Extracts were incubated for 2 h at room temperature with anti-V5 agarose beads (Sigma) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... a maximum of 600 ng of DNA per accession was sheared at 4°C to a target average length of 400 bp using a Bioruptor® Pico sonication device (Diagenode, Denville, NJ, USA). Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were sonicated for 30 minutes at 4°C (30 seconds ON, 30 seconds OFF, output setting high) using a water bath sonicator (Diagenode Bioruptor® Plus; Cat. No. B01020001), transferred to new 1.5 ml tubes ...
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bioRxiv - Neuroscience 2023Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Neuroscience 2024Quote: ... α-Synuclein PFFs were vortexed and diluted with DPBS to 2 mg/mL and sonicated in a cooled bath sonicator at 9°C (Diagenode Bioruptor® Pico ...
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bioRxiv - Genomics 2022Quote: ... H3K36me3 (Diagenode, C1541092-10), TFAP2A (Abcam ...
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bioRxiv - Neuroscience 2023Quote: ... The protein was then incubated at 37 °C for 120 hours with constant agitation at 200 rpm and the product sonicated 10 min at 10 °C (0.5 min on/0.5 min off) using a Biorupter Pico (Diagenode). Aggregates were flash-frozen with liquid nitrogen in single-use aliquots for storage at -80 °C.
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bioRxiv - Molecular Biology 2024Quote: ... followed by 10 min sonication (Diagenode Bioruptor). The extracts were then precleared ...
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bioRxiv - Molecular Biology 2023Quote: ... or 10 µg anti-IgG antibody (Diagenode). The next day ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... SDS lysates were boiled at 95°C for 10 minutes and then 10 cycles in a Bioruptor Pico (Diagenode) of 30 seconds on and 30 second off per cycle ...
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bioRxiv - Neuroscience 2020Quote: ... PFFs are diluted in PBS and sonicated for 10 cycles (1 sec ON, 30 sec OFF, high intensity) in a bath sonicator at 10°C (BioRuptor; Diagenode).
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bioRxiv - Genomics 2021Quote: ... 0.3% NP-40, 10% glycerol, 1XPIC) and sonicated for 10 cycles (30 s on, 30 s off) in a Bioruptor (Diagenode). SDS loading dye was added and the samples were boiled before immunoblotting.
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bioRxiv - Physiology 2024Quote: ... followed by heating to 95 °C for 10 min and sonication (Bioruptor, 10 cycles, 30 s on/off, Diagenode, Belgium). After equilibrating to room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and extracts were sonicated for 10 min (Diagenode Bioruptor ...
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bioRxiv - Developmental Biology 2024Quote: ... or 10 µg of IgG antibody (Diagenode, C15410206) as control ...
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bioRxiv - Bioengineering 2024Quote: ... Tagmentase (Tn5 transposase) - unloaded (Diagenode Cat# C01070010-10) was purchased from Diagenode and recombinant Tn5 protein was prepared in-house ...
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bioRxiv - Cell Biology 2020Quote: ... Upon sonication (Bioruptor, Diagenode, 10 cycles, 30 sec each) and centrifugation ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated for 10 cycles in a Bioruptor sonicator (Diagenode), and then centrifuged at 17000 xG for 20 min to pellet tissue debris ...
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bioRxiv - Genetics 2020Quote: ... and extracts were sonicated for 10 min (Diagenode Bioruptor). Extracts were then diluted 1:4 in RSB with 0.5% Triton X-100 (RSB + T ...
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bioRxiv - Molecular Biology 2023Quote: ... After 10 cycles of sonication using Bioruptor Pico (Diagenode), supernatants were cleared of debris by centrifugation at maximum speed at 4°C.
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bioRxiv - Molecular Biology 2024Quote: ... followed by 10 sec sonication with a Bioruptor (Diagenode) at low energy and incubation with 10 U Benzonase (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... PFFs were sonicated on high power (10 cycles of 30 sec on and 30 sec off) at 10°C with the Diagenode Bioruptor Plus (Diagenode B01020001, Denville, NJ, USA) prior to use.
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bioRxiv - Bioengineering 2024Quote: Unloaded Tn5 transposase proteins were acquired from Diagenode (#C01070010-10). Tn5 transposase was then loaded with oligos containing mosaic ends incorporating 13 base-pair unique molecular identifiers (UMIs ...
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bioRxiv - Molecular Biology 2023Quote: ... Unloaded Tn5 enzyme can be purchased from Diagenode (Cat:C01070010-10) and all sequences used for loading Tn5 are contained in Supp ...
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bioRxiv - Cell Biology 2023Quote: ... by sonication (Bioruptor, 10 cycles, 30s on/off, Diagenode, Belgium). Proteins were subsequently reduced by 10min incubation at 95°C and alkylated with 10 mM chloroacetamide for 30min at 37 °C ...
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bioRxiv - Evolutionary Biology 2023Quote: ... then sheared at 10 kb using the Megaruptor1 system (Diagenode). Using SMRTBell template Prep Kit 1.0 and SMRTbell Barcoded Adaptater kit 8A or 8B kits (PacBio) ...
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bioRxiv - Developmental Biology 2020Quote: ... sonicated with Biorupter® Sonicator for 10 cycles (Cat#B01020001, Diagenode), and centrifuged at 13,000 rpm for 10 min ...
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bioRxiv - Immunology 2021Quote: ... 10 mM EDTA (pH 8.0)) and sonicated in TPX microtubes (Diagenode) in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Genomics 2020Quote: ... and 1 EDTA-free protease inhibitor tablet (Roche)/10 mL in H2O) before sonication with a Bioruptor Pico (Diagenode) for 5 minutes (30 seconds on ...
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bioRxiv - Neuroscience 2020Quote: ... medium intensity in a bath sonicator at 10 C (BioRuptor; Diagenode) and then rotated at 4 C for 20 minutes ...
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bioRxiv - Physiology 2020Quote: ... by sonication (Bioruptor, 10 cycles, 30 seconds on/off, Diagenode, Belgium) and proteins were digested as described previously (Ahrne et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... Sonication (10 cycles, 30sec on/off) was performed with Picoruptor (Diagenode) according to the Diagenode recommendation ...
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bioRxiv - Genomics 2023Quote: ... The samples were fragmented to 10 kb using Megaruptor 2 (Diagenode). For each fragmented sample ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were sonicated for 10 cycles with Bioruptor Pico (Diagenode) until chromatin was sheared to an average length of 200 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... sonicated for 10 cycles in a Bioruptor Pico™ sonicator (Diagenode) and diluted 1:1.5 ratio in equilibrium buffer (10mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... by sonication (Bioruptor, 10 cycles, 30 seconds on/off, Diagenode, Belgium) and proteins were digested as described previously70 ...
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bioRxiv - Microbiology 2024Quote: ... Lysates were sonicated for 10 cycles (30s on/ 30s off; Diagenode). Protein concentration was measured using the BCA assay (Thermo Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and sonicated 10 times 30s on/off in a Bioruptor sonicator (Diagenode). Lysates were diluted 10-fold and quantified by BCA assay against a BSA standard curve in diluted lysis buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin was immunoprecipitated with 10 ug of H3K27Ac antibody (Diagenode cat# C15410196), 10 ug of ASCL1 antibody (abcam ab74065 ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sonication (10 minutes, every 30 seconds) (Bioruptor Pico Diagenode, Belgium). Next ...
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bioRxiv - Biochemistry 2024Quote: ... The ultrasonic pyrolysis cycle included 10-sec ultrasonic pyrolysis (Diagenode, Seraing, Belgium) and 30-sec interval ...
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bioRxiv - Genomics 2020Quote: ... total on-time 10 min) with a Biorupter® Pico Sonicator Device (Diagenode). 5 - 33 μg of sonicated chromatin was incubated with 4 μg ChIP-validated CTCF antibody (Active Motif ...