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Citations for Diagenode :
1 - 50 of 342 citations for tert Butyl 10 oxo 4 9 diazaspiro 4.5 decane 4 carboxylate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
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bioRxiv - Genetics 2020Quote: ... Resuspended pellets were shaken for 10 minutes at 4°C and lysed by sonication in a 4°C water bath (Diagenode Biorupter) for five minutes (30 seconds on/30 seconds off) ...
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bioRxiv - Genomics 2021Quote: ... sonicated for 10 cycles at 4°C using BioruptorPico device from Diagenode. For immunoprecipitation of H3K4me1 ...
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bioRxiv - Microbiology 2023Quote: ... 570 µM PMSF) for 10 min at 4 °C with sonication (Bioruptor, Diagenode, 10 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated 10 minutes at 4°C and then sheared using a Bioruptor (Diagenode, Belgium) for 4 times at 5 cycles and followed by 2 cycles at 30″ ON/OFF high power ...
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bioRxiv - Cell Biology 2021Quote: ... The homogenized samples were submitted to 10 cycles of 10 s at maximal intensity at 4⁰C in a sonicator bath (Bioruptor, Diagenode, USA). Then the homogenates were centrifuged at 10,000 g for 10 min at 4⁰C and the supernatants (NP40-soluble fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Genetics 2021Quote: ... incubated on ice for 10 min and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles with 30 s ON and 30 s OFF ...
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bioRxiv - Genetics 2023Quote: ... or 4 µg H3K9me3 antibody (C15410193, Diagenode) coupled to 30µL beads was added to 400µL soluble chromatin (Diagenode H3K9me3 antibody was used in Figure 2E only ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
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bioRxiv - Cell Biology 2024Quote: ... 150 mM NaCl and FPIC) for sonication for 4 min (4 x (30 s ON, 30 s OFF)) (Bioruptor, Diagenode), yielding genomic DNA fragments with a bulk size of 150–500 bp ...
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bioRxiv - Microbiology 2020Quote: ... high power at 4°C (Bioruptor Plus, Diagenode)] ...
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bioRxiv - Cancer Biology 2021Quote: ... lysed in 1% SDS containing buffer supplemented with 1x protease and phosphatase inhibitors, and sonicated for 4 x 10 cycles (30 sec on, 30 sec off) in temperature-controlled Bioruptor 300 (Diagenode). Debris was removed by centrifuging at 14,000 rpm at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... was then added at a final concentration of 1 % for 15 min at RT and samples were sonicated over 20 min (10 × 30 s pulses) at 4°C to cleave genomic DNA (Bioruptor, Diagenode). Anti-MAZ antibody was from Bethyl Laboratories (A301-652A) ...
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bioRxiv - Developmental Biology 2021Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Developmental Biology 2020Quote: ... Tubes were sonicated for 10 mins (sonication cycle: 30 sec ON, 30 sec OFF) at 4 °C in a Bioruptor ultrasonication bath (Diagenode) at high energy setting ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were sonicated at 4°C for 10 min (30 sec ON, 30 sec OFF) at medium frequency (Bioruptor Diagenode). Then ...
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bioRxiv - Cell Biology 2022Quote: ... cells were sonicated for 10 cycles of 30 sec “on” and 30 sec “off” at 4°C with Bioruptor (Diagenode) and the protein concentration was measured using Bradford protein assay (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 x 106 flow-sorted chromosomes were pelleted at 10,000 g for 10 min at 4 °C before tagmentation in a 50 μl reaction (2.5 μl Tagmentase (loaded Tn5 transposase, Diagenode, C01070012), 25 μl 2X Tagmentation buffer (Diagenode ...
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bioRxiv - Immunology 2021Quote: ... in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...
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bioRxiv - Physiology 2020Quote: ... and sonicated at 4 degrees using Bioruptor sonicator (Diagenode) for 20 cycles on high setting ...
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bioRxiv - Genomics 2021Quote: ... Per ChIP 4 µl RARα antibody (serum, Diagenode C15310155) or 2 µg IgG control (Diagenode C15410296 ...
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bioRxiv - Systems Biology 2021Quote: SARS-CoV-2 infected A549-ACE2 cell lysates were sonicated (10 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Protein concentration was adjusted based on Pierce660 assay supplemented with ionic detergent compatibility reagent ...
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bioRxiv - Systems Biology 2020Quote: ... Pellets were sonicated at 4°C using a Bioruptor (Diagenode) to shear the chromatin to a mean length of 300 bp by repeated cycles (16 cycles of 30 s ON and 30 s OFF) ...
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bioRxiv - Immunology 2020Quote: ... 30 s OFF at 4°C using a Bioruptor (Diagenode) with intermittent quick vortex and centrifugation using polystyrene tubes ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were sonicated at 4°C in Bioraptor Pico (Diagenode) for 10 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 s on/30 s off at 4° C (Bioruptor, Diagenode). One tenth of the sample was taken as input control ...
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bioRxiv - Microbiology 2021Quote: ... anti-Histone 4 rabbit (1:2,000; Diagenode Cat. No C15410156-50). HRP-conjugated anti-mouse and anti-rabbit antibodies were used (1:5,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were sonicated at 4°C using the Bioruptor Sonicator (Diagenode), with 6 cycles of 10 seconds on and 10 seconds off ...
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bioRxiv - Biochemistry 2021Quote: ... protease inhibitor cocktail) and sonicated in 4°C using Bioruptor (Diagenode) until lysate is clear ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were sonicated at 4°C using Bioruptor Pico (Diagenode B01080010) on high mode ...
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bioRxiv - Genomics 2020Quote: ... nuclei were isolated via sonication using a Bioruptor Pico (Diagenode; 9 cycles of 10 sec on and 30 sec off). Chromatin was then sheared in the recommended shearing buffer (27-30 cycles ...
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bioRxiv - Immunology 2022Quote: ... with a freshly added protease inhibitor cocktail and sodium vanadate and subjected to 9 cycles (30s ON-10s OFF) of ultrasonication (Bioruptor Plus; Diagenode). The sonicated samples are then spun down ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were sonicated in a 4 ⁰C water bath ultrasonicator (Bioruptor, Diagenode). The time of sonication was adjusted to yield short DNA fragments <500 bp (total 8 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were sonicated at 4 °C in a Bioruptor Waterbath Sonicator (Diagenode) for 5 cycles (30 sec on ...
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bioRxiv - Cell Biology 2021Quote: ... then sonicated at 4 °C in a Bioruptor Twin sonicating device (Diagenode) on HIGH 30 sec ON ...
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bioRxiv - Physiology 2021Quote: ... Probes were then homogenized at 4°C in Bioruptor Pico sonicator (Diagenode), using 30 sonication cycles (30 s on/off) ...
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bioRxiv - Cell Biology 2020Quote: ... 4 × 5 min cycle (30 s on/ 30 s off; Diagenode Bioruptor) with 4 min incubation on ice between sonication cycles ...
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bioRxiv - Cancer Biology 2022Quote: ... Libraries were constructed with CATS RNA-seq Kit (Diagenode C05010041 Lot#4) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and sonicated at 4°C using a Bioruptor Pico sonication device (Diagenode) for 10 cycles (30s ON/30s OFF ...
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bioRxiv - Biochemistry 2024Quote: ... The suspension was sonicated at 4°C with a Bioruptor (Diagenode, #B01020001) using conditions optimized to generate 200–500 bp chromatin fragments ...
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bioRxiv - Microbiology 2020Quote: ... 50 mM NaCl) and sonicated for 30 minutes at 4°C (Diagenode Bioruptor). Anti-di-glycine peptide beads (Cell Signalling #5562 ...
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bioRxiv - Cell Biology 2022Quote: ... Chromatin was incubated overnight at 4°C using 0.25µg of H3K9me3 (C15410193-Diagenode) per IP ...
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bioRxiv - Plant Biology 2021Quote: ... Sonication of chromatin was done in 4°C water sonicator (Diagenode Bioruptor Plus). Sonicated samples were first precleared with Protein A Agarose beads (Millipore #16-157 ...
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bioRxiv - Synthetic Biology 2021Quote: ... with following antibodies at 4 °C on a rotating wheel: H3K27me3 (Diagenode, C15410195), Ring1B (Cell Signaling ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154 ...