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Citations for Diagenode :
151 - 200 of 212 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: 5×105 fixed nuclei were sonicated to a 200-500bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP was (Diagenode C15410174 ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Plant Biology 2022Quote: ... Cells were ruptured by 5 × 15 sec sonication in an ice bath in Bioruptor UCD-200 (Diagenode) at medium intensity with 2 min breaks on ice ...
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bioRxiv - Genetics 2024Quote: ... 5 μg of genomic DNA from each treatment condition was sheared using a bioruptor sonication device (Diagenode) in low mode for two cycles (30s on + 60s off ...
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bioRxiv - Molecular Biology 2023Quote: ... and then sonicated for 5 min at high setting with 30sON/30sOFF in a Bioruptor sonicator (Diagenode) and centrifuged for 5 min at 19000g at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in 5 volumes of washing buffer and sonicated in a Bioruptor Plus (Diagenode) for 5 cycles of 30 seconds ON ...
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bioRxiv - Cancer Biology 2024Quote: ... Lysates underwent 5 minutes of sonication with on/off intervals of 30 seconds using a Bioruptor (Diagenode), followed by centrifugation at 20,000 g for 10 minutes to retrieve the protein supernatant ...
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bioRxiv - Cancer Biology 2024Quote: ... and sonicated for 5 cycles (30 s ON, 30 s OFF) with the Bioruptor Pico sonicator (Diagenode). Chromatin was cleared by centrifugation (13,000 rpm for 10 min at 4 C) ...
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bioRxiv - Developmental Biology 2021Quote: ... and subsequently sonicated for 3 cycles (30 seconds ON, 30 seconds OFF) on low settings using a Bioruptor® Plus (Diagenode, #B01020001). Samples were then centrifuged for 10 min at 10.000 g and supernatant was collected ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Cancer Biology 2024Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended nuclei were sonicated 5 times 30 sec on followed by 30 sec off using the Bioruptor (Diagenode). Insoluble proteins were removed by centrifugation for 10 min at 14,000 rpm at 4 °C.
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bioRxiv - Cancer Biology 2021Quote: ... Adapter-ligated DNA was subjected to immunoprecipitation with a primary monoclonal antibody against 5-methyl cytosine (Diagenode C15200081) using a previously published protocol [71] ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Cell Biology 2024Quote: ... Chromatin corresponding to 5 μg of DNA was incubated with 2 μg of anti-H3K4me3 (Diagenode; Cat#: C15410003) or anti-H3K9me3 (Diagenode ...
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bioRxiv - Plant Biology 2023Quote: ChIP assays were performed on leaves and axillary buds enriched samples of 3 weeks old plants using anti-H3K27me3 (Diagenode pAb-195-050) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Neuroscience 2021Quote: ... the adaptor-ligated DNA was used for immunoprecipitation (IP) with a mouse monoclonal anti-5-hydroxymethylcytosine antibody (Diagenode, C15200200). For this ...
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bioRxiv - Microbiology 2021Quote: ... Cells were lysed by sonication (5 cycles, each 30 s on and 30 s off) using a Biorupter (Diagenode). A centrifugation step (at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Crosslinking was terminated by the addition of 1/10 volume 1.25 M glycine for 5 min at room temperature followed by cell lysis and sonication (Bioruptor, Diagenode), resulting in an average chromatin fragment size of 200 bp ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Resuspended samples were boiled for 5 min at 95°C and sonicated for 15 min in the Bioruptor (Diagenode) at 30 sec on ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Molecular Biology 2023Quote: ... High Molecular Weight Genomic DNA (5 µg) was sheared with the 40 kb program using a Diagenode Megaruptor (Diagenode) to generate DNA fragments of approximately 30 kb ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... sonicating on high for 5 x 60 sec on/30sec off using the bioruptor pico sonication water bath (Diagenode) and finally disrupting twice with the tissuelyser (QIAGEN ...
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bioRxiv - Genomics 2024Quote: ... 5 μl of each annealed A and B transposome were combined with 10 μl of unloaded Tagmentase (Diagenode, C01070010), briefly vortexed ...
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bioRxiv - Cell Biology 2024Quote: ... After 2 × 5 cycles of sonication (30 s “ON”, 30 s “OFF”, High intensity) with a Bioruptor UCD200 (Diagenode), the chromatin solution was diluted 10-fold to a final volume of 3 mL with ChIP dilution buffer before IP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10.0 μg of high molecular weight purified genomic DNA (final volume of 120 μl) was sheared using the Diagenode Megaruptor 3 instrument (Diagenode Inc., Denville, NJ, USA) and a two-cycle shear method at speed 33 followed by speed 34 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Immunology 2020Quote: ... chromatin was sheared using a Bioruptor Pico Sonicator (5 cycles: 30 seconds ON - 30 seconds OFF, fragments 100 - 300 bp, Diagenode) and 10 % of sheared chromatin was kept as input control whilst 90 % was immunoprecipitated overnight using antibodies against H3K27ac ...
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bioRxiv - Cell Biology 2020Quote: ... assisted by sonication (5 cycles of 30 s ON, 30 s OFF at high power, Bioruptor Plus ultrasonic disintegrator, Diagenode). Protein concentration was measured by the BCA assay.
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bioRxiv - Developmental Biology 2022Quote: ... in VFG media for 10 minutes at room temperature followed by a 5-minute quench with glycine (in True MicroChIP kit, Diagenode) at room temperature ...
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bioRxiv - Genomics 2022Quote: ... and chromatin was fragmented into 1–5-kb fragments by sonication (high level, 33 cycles, 30 s ON, 50 s OFF) using a Bioruptor (Diagenode). Chromatin immunoprecipitation was used to enrich the complex fragments with magnetic beads of protein G (Thermo ...
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bioRxiv - Pathology 2021Quote: ... and fragmented by 5 cycles of sonication on a Bioruptor (per cycle: power = high, interval = 30s on/30s off, 5min; Diagenode) to facilitate efficient DNA clustering onto the Illumina sequencing platform ...
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bioRxiv - Molecular Biology 2023Quote: MeDIP-Seq libraries for Ion Torrent semiconductor sequencing were performed on the Ion Torrent PGM using a modified protocol from Ion Plus Fragment Library kit (Catalog # 4471252) combined with a 5-hydroxymethylcytosine (5hmC Kit, Catalog # AF-110–0016) immunoprecipitation kit (Diagenode) according to a previously optimized protocol (Guerrero-Bosagna & Jensen ...
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Bioengineering 2024Quote: ... The cell lysates were heated at 95[ for 5 minutes and sonicated for 15 min using a Bioruptor Plus (Diagenode). The supernatant was collected after centrifugation at 18,000 g for 30 min at 4[ ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell lysis was obtained by pulsed ultrasonication (5 cycles, 30” On, 30” Off, at High intensity using Diagenode Bioruptor 300). Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 ml of thawed OFs were sonicated with a closed-type sonication system (UCD-200T, Biorupter, Diagenode Inc., NJ, USA) and were sequentially centrifuged at 300 g for 10 min (model 5500 with tube 430829 ...
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bioRxiv - Cancer Biology 2023Quote: ... was added to the supernatant and sonicated for 30 s ON/OFF for 5 min at high power in Bioruptor (Diagenode). After centrifugation at 16,000 × g for 10 min at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Neuroscience 2024Quote: ... Cell extracts were then sonicated at 4°C for 5 cycles (30 seconds on / 30 seconds off) with the Bioruptor Pico (Diagenode), prior to proceeding with protein quantification using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: Cortex nuclei were purified as above and collected by centrifugation (500 g; 5 min) for immediate use in ATAC Library preparation (Tagmentase–loaded; Diagenode). The nuclei pellets were suspended in 25 μL 2xTagmentation Buffer (Diagenode ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...
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bioRxiv - Molecular Biology 2024Quote: ... and then sonicated with series of 30 s ON / 30 s OFF for 5 min on a Bioruptor device (Diagenode) and centrifuged at 16,000 g for 15 min at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Molecular Biology 2021Quote: ... A portion of sheared DNA (10%) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...