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Citations for Diagenode :
101 - 150 of 212 citations for 5 tert Butyl 3 isocyanatoisoxazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... They were sonicated for 5 cycles 30s ON/OFF in a Bioruptor sonicator (Diagenode). Samples were centrifuged ...
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bioRxiv - Molecular Biology 2024Quote: ... sonicated for 5 cycles (30 sec ON/30 sec OFF) in the Bioruptor (Diagenode) and centrifuged at 14000 x g for 2 min at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Cancer Biology 2020Quote: ... chromatin was sheared to 100-500bp fragments using the Bioruptor Pico sonicator for 3 cycles of 30 sec on/off in 0.5ml tubes (Diagenode). Quantitation of chromatin was performed using a qubit fluorimeter where at least 10 ng of chromatin were used per experiment ...
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bioRxiv - Plant Biology 2022Quote: ... the protocol involves fragmenting the HMW DNA to a mean fragment length of 20 kb using Megaruptor 3 (Diagenode), followed by DNA damage repair ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Neuroscience 2024Quote: ... the extracted DNA was sheared to a target size of 18-20 kb using the Megaruptor 3 (Diagenode, B060100003). After confirmation of correct sizing ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... by 30-min incubation on ice followed by sonicating for 3 min at medium power using a Bioruptor (Diagenode). Protein concentration was measured by Bradford assay (Bio-Rad ...
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... lysates were first sonicated for 3 cycles of 1 min pulse at 30 sec intervals in a Bioruptor (Diagenode) and then centrifuged at 4°C for 5 min at 1,000 g ...
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bioRxiv - Genomics 2024Quote: Long-read sequencing was facilitated by fragmenting genomic DNA using the Megaruptor 3 shearing kit (Diagenode SA, Seraing, Belgium). Fragments less than 5 kb were selectively removed using the AMPure PB beads size selection kit (Pacbio ...
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bioRxiv - Genomics 2024Quote: ... and one of two U2OS libraries were prepared by first treating 3 μg of DNA with the Short Fragment Eliminator Kit (ONT) according to the manufacturer’s protocol before shearing with the DNA Fluid+ Kit on a Megaruptor 3 (Diagenode) to a mean size of approximately 50k bp ...
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bioRxiv - Developmental Biology 2024Quote: ... The chromatin was sheared by 3 cycles of 30-s ON/30-s OFF on the Bioruptor Pico (Diagenode). The antibodies used were H3K27me3 (Millipore ...
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bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
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bioRxiv - Plant Biology 2021Quote: Methylated DNA immunoprecipitation (MedIP) assays were performed using anti 5-mC (Diagenode Mab-081-100) and anti-IgG (Abcam ab6702) ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were sonicated (5× cycles of 30 s on and 30 s off; Bioruptor, Diagenode) and clarified by centrifugation (16,000 × g for 15 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated (5 cycles of 30 s ON, 30 s OFF, high power, Bioruptor Plus, Diagenode), spun for 10 min at 16,000 × gmax to remove any insoluble material ...
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bioRxiv - Genomics 2022Quote: ... 5 ug of genomic DNA was sheared to approximately 15-20 kb using Megaruptor3 (Diagenode). The sheared DNA was treated with DNA Prep to remove single-stranded ends ...
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bioRxiv - Cell Biology 2023Quote: ... nuclei were sonicated in ice water for 5×30 s (power: middle, Bioruptor Plus, Diagenode). Samples were centrifuged for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 30 min at 4°C with rocking and then sonicated (3 x 30 sec interval, high setting in a Bioruptor water bath sonicator, Diagenode). Lysates were cleared by centrifugation at 13,000 g for 15 min at 4°C ...
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bioRxiv - Genetics 2020Quote: ... The top 100 µl sperm suspension was sonicated at the medium level for five times with 3 seconds each (Bioruptor UCD-200; Diagenode). An aliquot of 2 µl sperm HTF suspension was mixed immediately with 50 µl of 4% PVP (Sigma ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Molecular Biology 2021Quote: ... the nuclear lysate was sonicated for 3 cycles of 30 seconds ON / 30 seconds OFF using a Bioruptor Pico (Diagenode) to ensure solubilisation of chromatin and then spun 18,000g for 10 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were sonicated briefly (3 cycles, 12 s on, 12 s off) at a high setting in a Bioruptor (Diagenode) sonicator ...
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bioRxiv - Genomics 2024Quote: HMW DNA was sheared to 20 kb using Covaris G-Tubes (for shallow-depth sequencing with MinION) or using the Megaruptor 3 (Diagenode; for high-depth sequencing with PromethION) ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: 15 μg of DNA was cleaned with a 1X AMPure PB beads (Pacific Biosciences) cleanup and sheared to a target size of 14 kilobases (kb) using the Megaruptor 3 instrument (Diagenode) with the following settings ...
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bioRxiv - Microbiology 2024Quote: ... The cells were incubated for 20 min on ice and lysed by sonication (3 cycles, 30 sec high-power pulse) using a Bioruptor Plus (Diagenode). After centrifugation (10,000 x g ...
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bioRxiv - Genomics 2024Quote: ... The DNA was quantified using Qubit and the fragment length was assessed with the Fragment Analyzer (HS large fragment kit) and then sheared to a length of 24 kb using the Megaruptor 3 (Diagenode) (45/200/30) ...
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bioRxiv - Genomics 2024Quote: ... 6.4 ug of HG008-T and 6.1 ug of HG008-N-P were sheared separately on a Megaruptor 3 (Diagenode, B06010003) to a target size of 15 to 20 kb ...
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bioRxiv - Neuroscience 2024Quote: ... were subjected to 3 cycles of sonication (30sec ON/30sec OFF for 10 min, at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to the sonicated lysate to a final concentration of 1% ...
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bioRxiv - Neuroscience 2024Quote: ... were subsequently subjected to 3 cycles of sonication (30s ON/30s OFF for 10 minutes at 200W, high setting, Bioruptor, Diagenode). Triton X-100 was added to a final concentration of 1% ...
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bioRxiv - Genetics 2024Quote: ... We sheared the high molecular weight (HMW) DNA to achieve consistent and narrow size ranges to 20kb using the Megaruptor 3 Shearing kit (E07010003, Diagenode). Megaruptor 3 was performed using customized settings ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Molecular Biology 2021Quote: ... sonicated with a series of 30s ON / 30s OFF for 5 min on a Bioruptor (Diagenode), and centrifuged at 16,000 g for 5 min at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... pH 8.0) and sonicated for 5 min (30 sec ON/ 30 sec off) with Bioruptor (Diagenode). Total protein was measured with a Bradford assay (Bio-Rad ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were heated at 72 °C for 5 min and sonicated using a Bioruptor (Diagenode Inc) with fifteen 30 s on/off cycles at maximum intensity ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Systems Biology 2023Quote: ... single-stranded DNA mixture was incubated with 5-mC antibody provided by MagMeDIP-seq Package (Diagenode), followed by 14-16 cycles of library amplification ...
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bioRxiv - Cancer Biology 2023Quote: ... was used and 5 cycles sonicated then (Bioruptor® Plus and Bioruptor® Next Gen, Diagenode). Then supernatant was diluted to dilution buffer 2.5 times ...
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bioRxiv - Cell Biology 2024Quote: ... the reverse crosslinking (5 h at 65°C) and elution were performed using IPure kit (Diagenode). The final elution was performed in 60 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... buffer with added protease and phosphatase inhibitors and sonicated on high for 30 seconds off 30 seconds on for 3 minutes (Diagenode Bioruptor). The protein concentration was determined using the Micro BCA TM protein assay kit (Thermo Fisher) ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...