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Citations for Diagenode :
1901 - 1950 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The resulting chromatin was sheared in NP buffer using a sonicator bath (Bioruptor Pico; Diagenode, Ougrée, Belgium) for 30 minutes at maximum output and 30 seconds on/off cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL of MgCl2 (Diagenode ChIPmentation kit for Histones ...
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bioRxiv - Molecular Biology 2021Quote: ... 1μL SYBR green 10X (Diagenode kit), 1.3 μL of molecular biology grade water and 5μL of 2xPCR NEB master mix ...
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bioRxiv - Molecular Biology 2021Quote: In the ChIPmentation Kit for Histones (Diagenode, Cat. No. C01011009) the suggested strategy is to sequence one sample immunoprecipitated with a control IgG and to use it for sequencing normalization instead of the classical input which cannot be treated exactly the same way as the immunoprecipitated samples ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were sonicated for 10 min at the high intensity setting (30 sec on/off) in a Bioruptor (Diagenode). The whole cell extract was adjusted to 25 mM MgCl2 and 5 mM CaCl2 and 1 µL DNAse I (30 U ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were sonicated with the Bioruptor Pico (Diagenode, Cat. No. B01080010) for 5 cycles (30s ON and 30s OFF) ...
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bioRxiv - Molecular Biology 2021Quote: ... The chromatin samples were sonicated for 7 cycles (30 s ON, 30 sec OFF, at High output) in a Bioruptor (Diagenode). The samples were heated to 95°C for 5 min and stored at -20°C ...
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bioRxiv - Molecular Biology 2021Quote: ... For each sample the Ab coating mix was done with 4 μl anti-H3K4me3 (Diagenode, Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... The cell lysate was sheared with Biorupter (Diagenode) with 3 cycles of 30 seconds ON/OFF for 10 min each at high power ...
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bioRxiv - Molecular Biology 2021Quote: ... sonicated (Diagenode Bioruptor Pico) and resolved by SDS-PAGE.
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bioRxiv - Molecular Biology 2021Quote: ... and 1 mM DTT) and sonicated for 3x 10 seconds using a Bioruptor Pico (Diagenode). Cells were then rotated at 4°C for 20 mins before the lysates were diluted with 500 µl of no salt buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... Anti-TET2 (C15200179, Diagenode).
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bioRxiv - Molecular Biology 2021Quote: ... Sonication was performed on a Bioruptor® Pico sonication device (Diagenode) with ten 30-sec pulses with a 30-sec break between pulses ...
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bioRxiv - Molecular Biology 2021Quote: ... Sonication was performed on a Bioruptor® Plus sonication device (Diagenode) with two 15-sec pulses at 50% amplitude with a 1-min break between pulses ...
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bioRxiv - Molecular Biology 2021Quote: ... Cross-linking was reversed at 65°C overnight and immunoprecipitated DNA was recovered using the IPure Kit (Diagenode). Genomic regions of interest were identified by realtime PCR (qPCR ...
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bioRxiv - Molecular Biology 2021Quote: ... H3K27ac (Diagenode; C15410174), Ezh2 (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... H3K4mel (Diagenode; C15410037), H3K27ac (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were lysed using Bioruptor (10 cycles, 30 sec ON and 30sec OFF, Diagenode). The Supernatants were incubated with 50μl streptavidin-magnetic beads at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... YY1 (Diagenode; C15410345), H3K4mel (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... Ezh2 (Diagenode; C15410039).
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bioRxiv - Biochemistry 2021Quote: ... Lysates were first sonicated for 6 cycles of 30 sec on/30 sec off under low power setting with Bioruptor™ Twin (Diagenode), and then centrifuged at 17,000 × g for 10 min at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: Cellular human and viral material in sputum deposited on glass slides was lysed in 50 μl 2% SDS dissolved in 50 mM Tris/HCl (pH 8.2) followed by sonication in a Bioruptor Pico (Diagenode). Proteins were digested using the SP3 protocol as described below.
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Molecular Biology 2021Quote: ... with 437.5 mg sonication beads (Diagenode, C03070001) for 6 cycles (1 min on/1 min off ...
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bioRxiv - Molecular Biology 2021Quote: ... sample DNA was ethanol precipitated and sheared in 500 µL sonication buffer (50 mM Tris pH 8.0, 0.1% SDS, 10 mM EDTA) on a Bioruptor Pico sonicator (Diagenode). DNA was then concentrated on Amicon ultra 0.5 30K filter units (Millipore) ...
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bioRxiv - Molecular Biology 2021Quote: ... H3K27me3 (Diagenode p069-050) 1:1000 ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromatin was sheared by sonication in 15 ml Bioruptor tubes (Diagenode, C01020031) with 437.5 mg sonication beads (Diagenode ...
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bioRxiv - Molecular Biology 2021Quote: ... for 6 cycles (1 min on/1 min off) on a Bioruptor Pico sonicator (Diagenode). For each ChIP reaction 4 % HEK293T-derived human spike-in lysate was combined with mESC lysate and incubated in 1x IP buffer (50 mM HEPES/KOH pH 7.5 ...
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bioRxiv - Genomics 2021Quote: ... Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg, C15410069, Diagenode; H3K27ac 1µg, ab4729, Abcam). As wildtype FLI1 or ERG are not expressed in EWSR1-FLI1 or EWSR1-ERG positive EwS cell lines ...
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bioRxiv - Genomics 2021Quote: ... Cells were lysed in two steps with the Diagenode iDeal ChIP-seq kit for transcription factors (Diagenode, Seraing, Belgium).
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bioRxiv - Genomics 2021Quote: ... and sheared DNA was added to antibodies coupled to washed magnetic beads (anti-FLI1 2 µg, ab15289, Abcam; anti-ERG 2 µg, ab92513, Abcam; anti-H3K4me3 1.4 µg, C15410003, Diagenode; H3K27me3 2.9 µg ...
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bioRxiv - Genomics 2021Quote: ... 0.5 % NP-40) and evenly split into pre-cooled 1.5 ml Bioruptor tubes (Diagenode). After sonication (15 sec on/ 30 sec off ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Genomics 2021Quote: ... 8 µg of adequately sheared chromatin was subjected to immunoprecipitation using iDeal ChIP-seq kit for Histones (Diagenode, C01010050) with 2 µg of antibodies against histone modifications or 10 µg of custom RPB1 antibody ...
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bioRxiv - Genomics 2021Quote: ... A total of 10 μg of DNA was purified then sheared at 40kb using the Megaruptor system (Diagenode). Using SMRTBell template Prep Kit 1.0 (PacBio) ...
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bioRxiv - Genomics 2021Quote: ... pAb-037-050 (Diagenode)
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bioRxiv - Genomics 2021Quote: ... After overnight incubation with DiaMag magnetic beads (Diagenode, Inc.) and antibodies ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We reserved 10% of the pooled fragmented sample for the input (fragment control) and used the remaining 90% to perform the chromatin pull-down (ChIP sample, target antibody: H3K9me3 polyclonal classic Diagenode C15410056) according to the 100,000 cell specifications ...
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bioRxiv - Genomics 2021Quote: DNA was sheared with Bioruptor Plus sonication device (Diagenode). Cell debris was spun down ...
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bioRxiv - Cancer Biology 2021Quote: ... sonicated 5 minutes (Bioruptor plus, Diagenode, 5 cycles high, 30 s on, 30 s off) and incubated at 37 °C for 60 min ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were processed as for ChIP-qPCR except 4 cycles of 60 sec on Fastprep Bio-Pulveriser FP120 and two rounds of 30 cycles 30 sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH were performed ...
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bioRxiv - Cell Biology 2021Quote: ... DNA was fragmented with 2 rounds of 30 cycles 30sec ON-30sec OFF of sonication with Bioruptor Plus (Diagenode) set on HIGH and purified with Qiagen MinElute kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Genomics 2021Quote: ... The RRBS libraries were prepared using the Diagenode Premium RRBS Kit (#C02030032, Diagenode, Denville, NJ) with the following exceptions ...
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bioRxiv - Genomics 2021Quote: Unloaded Tn5 transposase (C01070010) was purchased from Diagenode, and the transposome was assembled following manufacturer’s guidelines ...
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bioRxiv - Genomics 2021Quote: ... H3K4me3 (Diagenode #C15410003, 1.4 µg/ChIP), H3K27me3 (Diagenode #C15410195 ...
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bioRxiv - Genomics 2021Quote: ... H3K27ac (Diagenode #C15410196, 1.4 µg/ChIP), H3K4me3 (Diagenode #C15410003 ...
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bioRxiv - Genomics 2021Quote: ... H3K27me3 (Diagenode #C15410195, 1.4 µg/ChIP), RNA Polymerase II 8WG16 (Covance #MM2-126R ...
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bioRxiv - Genomics 2021Quote: ... and H3K27ac (#C15410196, Diagenode), was performed at the Dana-Farber Cancer Institute using the protocol described previously 32,63.
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bioRxiv - Immunology 2021Quote: ... 10 mM EDTA (pH 8.0)) and sonicated in TPX microtubes (Diagenode) in a 4°C-cooled water bath sonicator (Diagenode Bioruptor ...