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Citations for Diagenode :
901 - 950 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Protease Inhibitor Cocktail) and sonicated by using a Bioruptor® Plus sonication device (Diagenode) to obtain the desired DNA fragment size (enriched at 500 bp) ...
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bioRxiv - Plant Biology 2023Quote: ... DNA was purified according to the IPure kit v2 Kit manual (Diagenode, C03010015). The amount of immunoprecipitated DNA was quantified by qPCR ...
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bioRxiv - Plant Biology 2023Quote: ... and cOmplete protease inhibitor cocktail tablets) and proceeded for sonication to shear the chromatin (∼ 500 bp to 1 kb size fragments) using Bioruptor Plus (Diagenode, USA) set at low mode and 45 cycles of 30 sec ON ...
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bioRxiv - Plant Biology 2023Quote: ... The chromatin solution was sonicated using a Biorupter Plus (Diagenode) with 10 cycles of 30 sec pulse/90 sec cooling at high power at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µg of either IgG control antibody (Diagenode; C15410206) or of a specific antibody were applied ...
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bioRxiv - Molecular Biology 2023Quote: ... anti-H3K3me2 (Diagenode; C15410035), and anti-H3K4me3 (Diagenode ...
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bioRxiv - Molecular Biology 2023Quote: ... and anti-H3K4me3 (Diagenode; C15410003).
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13.000 rpm and 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the Microplex library preparation kit v2 (Diagenode, C05010012) was used for indexed sequencing library preparation with chromatin immunoprecipitated DNA ...
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bioRxiv - Molecular Biology 2023Quote: Chromatin-immunoprecipitation (ChIP) for ChIP-qPCR was performed according to the One-day ChIP kit protocol (Diagenode; C01010080). Beads were exchanged to Dynabeads™ Protein A (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed by sonication (Bioruptor, Diagenode, high intensity for 10 minutes with an ON/OFF cycle of 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043) ...
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bioRxiv - Cell Biology 2023Quote: ... 1.7ug Tn5 (Diagenode C01070010) previously loaded with nextera adapters loaded ...
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bioRxiv - Cancer Biology 2023Quote: ... The tissues were homogenized using a Bioruptor standard water bath sonicator (Diagenode, UCD-200) with the following settings ...
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bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043), 0,1% Tween-20 ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by water bath sonication (Diagenode Bioruptor Plus) to reduce sample viscosity and a 5-minute incubation at 95°C to ensure complete protein denaturation ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003 ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...
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bioRxiv - Molecular Biology 2023Quote: ... a rabbit anti-H3K4me3 antibody (1:100; Diagenode, C15410003) was used ...
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bioRxiv - Plant Biology 2023Quote: ... Each genomic DNA was subjected to 20-kbp fragmentation using the Megaruptor2 (Diagenode, Belgium). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: Bicone suspensions were dispensed into 1.5 mL polymethylpentene (TPX) tubes (Diagenode) and allowed to equilibrate overnight at ambient conditions ...
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bioRxiv - Microbiology 2023Quote: ... 30 sec on/off each (Diagenode sonicator). Cell lysates were centrifuged (21,000 x g ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cancer Biology 2023Quote: The ChIP reaction was performed using the iDeal ChIP-seq kit (Diagenode, Cat# C01010055). Samples were pre-cleared using the beads provided in the kit and incubated overnight at 4°C with 5 µg of commercially available antibodies against lamin A/C (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by sonication (10 minutes, every 30 seconds) (Bioruptor Pico Diagenode, Belgium). Next ...
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bioRxiv - Cell Biology 2023Quote: ... with sonication using a Bioruptor® sonication system (Diagenode Inc ...
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bioRxiv - Cell Biology 2023Quote: ... TPCK 5 μg/mL) and sonicated on a Bioruptor Pico (Diagenode) for 15 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode; #C30010016) to generate 100–400 bp DNA fragments ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclear extracts were sonicated on a Bioruptor Pico (Diagenode) for 10 cycles 30 sec ON/30 sec OFF in 1.5 mL Diagenode tubes (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... cells were disrupted using a sonicator (Bioruptor, Diagenode) to produce an average DNA fragment size of 250 bp ...
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bioRxiv - Cell Biology 2023Quote: ... and H3K27me3 (Diagenode; #C15410195; 1:200). After washing ...
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bioRxiv - Cell Biology 2023Quote: ... H4K20me1 (Diagenode; #C15410034; 1:500), H3K9me3 (Diagenode ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1X protease inhibitor (Genetix) followed by DNA fragmentation through sonication to an average fragment length of ∼500 bp using Bioruptor (Diagenode) and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were lysed and chromatin was prepared using the Chromatin EasyShear Kit - High SDS (Diagenode; Cat# C01020012), and shearing of the chromatin was achieved using the Bioruptor with four rounds of ten cycles of 30 seconds “ON„ and 30 seconds “OFF„ at HIGH power setting (position H) ...
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bioRxiv - Cancer Biology 2023Quote: ... chromatin was prepared using the Chromatin EasyShear Kit - Low SDS (Diagenode, Cat# C01020013) and sonicated using an E220 focused ultrasonicator (Covaris).
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bioRxiv - Cancer Biology 2023Quote: ... ATAC-seq library preparation involved PCR amplification of the purified DNA fragments using Diagenode primer indices (Diagenode, Cat# C01011034). Amplification cycles were adjusted and libraries were purified using AMPure beads ...
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bioRxiv - Developmental Biology 2023Quote: ... these were then lysed in 1% SDS buffer and the chromatin was sheared into 300-1000 bp fragments by sonicating for 40 cycles (30 sec on and 45 sec off) using a bioruptor (Diagenode). Immunoprecipitation (IP ...
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bioRxiv - Cancer Biology 2023Quote: ... The released chromatin was sonicated with Bioruptor Plus sonicator (Diagenode) and was centrifuged at 10.000 g for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... For protein extractions, the chromatin fraction was sonicated for 4 min (30s-on/30s-off, “high power”) on the Bioruptor Plus (Diagenode). Proteins’ content was quantified with the Thermo Fisher Scientific− BCA Protein Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: ... using the iDeal ChIP-seq kit (Diagenode). Libraries were prepared using the Ovation Ultralow V2 DNA-Seq library preparation kit (NuGen ...
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bioRxiv - Molecular Biology 2023Quote: ... and libraries were generated with the MicroPlex Library Preparation Kit (Diagenode: C05010001). Completed libraries were sent to Genome Quebec for 100bp paired end sequencing on an Illumina NovaSeq 6000 S4.
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bioRxiv - Molecular Biology 2023Quote: ... or anti-PolII (Diagenode C15200004 and sc-55492) antibody was added and incubated overnight at 4°C on a rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... boiled for 5 min at 95°C 1000rpm in 2% SDC buffer (2% SDC, 100 mM Tris-HCl pH=8.5) and sonicated (Diagenode Bioruptor, 15 * 30s at high intensity). Protein concentration was determined via BCA Protein Assay (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: ... using a Bioruptor Pico (Diagenode). After incubation for 1 hr at 4°C and then 1 hr at RT with or without RNase A or PGA ...
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bioRxiv - Molecular Biology 2023Quote: ... using a Bioruptor Pico (Diagenode). Sonicated extracts were diluted 1:5 in IP buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Molecular Biology 2023Quote: ... then sonicated with a Bioruptor (Diagenode). The sonicated samples were centrifuged at 16,000 g for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... After this time, samples were sonicated twice (7 cycles 30s on, 30s off) with a Bioruptor Plus sonicator (Diagenode). In between the two sonication cycles samples were incubated at 12°C with 0.8ul of Benzonase (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein concentrations of lysates were determined after 15 min of sonication (Bioruptor, Diagenode, cycles of 30 s) using the Pierce BCA Protein Assay (Thermo) ...
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bioRxiv - Molecular Biology 2023Quote: ... ChIP-Seq libraries were made using Microplex library preparation kit v2 (Diagenode) and sequenced using Illumina NextSeq 550 at the Genomics Core Facility Marburg (Center for Tumor Biology and Immunology ...
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bioRxiv - Molecular Biology 2023Quote: ... the samples were sonicated two times for 7 min each using a precooled Bioruptor® (Diagenode). The samples were centrifuged for 10 min at 13,000 rpm and 4 °C ...