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Citations for Diagenode :
851 - 900 of 2740 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... followed by water bath sonication (Diagenode Bioruptor Plus) to reduce sample viscosity and a 5-minute incubation at 95°C to ensure complete protein denaturation ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043) ...
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bioRxiv - Cell Biology 2023Quote: ... 1.7ug Tn5 (Diagenode C01070010) previously loaded with nextera adapters loaded ...
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bioRxiv - Cell Biology 2023Quote: ... The pelleted nuclei were resuspended in 50 µl transposase reaction mix (1X Diagenode Tagmentation buffer (Diagenode C01019043), 0,1% Tween-20 ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatin was fragmented by a Bioruptor (Diagenode) programed at high intensity for 3 x 7.5 min (cycles of 30 s on and 30 s off ...
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bioRxiv - Microbiology 2023Quote: ... 30 sec on/off each (Diagenode sonicator). Cell lysates were centrifuged (21,000 x g ...
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bioRxiv - Plant Biology 2023Quote: ... Nuclei were resuspended in Nuclei Lysis Buffer and chromatin was sonicated using a water bath Bioruptor Pico (Diagenode; 30 s on / 30 s off pulses ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was sonicated in an ultrasonic bath (Diagenode, Bioruptor pico) to an average length of 200–250 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were recovered and chromatin extracted and sonicated (Bioruptor, Diagenode) to an average DNA length of 500 bp ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated chromatin was fragmented by sonication at 4°C for 10 minutes using Bioruptor (Diagenode). Run-on reactions were performed at 37°C for 5 minutes using biotinylated nucleotides (5 mM Tris-HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K4me3 (Diagenode #C15410003, 1 μg), H3K27me3 (Diagenode #C15410069 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibodies against H3K27ac (Diagenode #C15410174, 1.2 μg), H3K4me3 (Diagenode #C15410003 ...
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bioRxiv - Developmental Biology 2023Quote: ... H3K27me3 (Diagenode #C15410069, 1.5 μg), p63 (Santa Cruz #H129 ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genomics 2023Quote: ... A portion of sheared DNA (10 %) was kept as input and the rest of the sheared DNA was immunoprecipitated with α-5-methylcytosine antibody (Diagenode), bound to magnetic beads ...
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bioRxiv - Immunology 2023Quote: ... and sonicated on ice for 5-10 min using a Bioruptor® sonicator (Diagenode). The cell lysate was centrifuged at high speed (16,000 g ...
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bioRxiv - Cell Biology 2023Quote: ... H3K4me3 (1:1000; Diagenode C15410003), H3S10ph clone MC463 (1:2000 ...
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bioRxiv - Biophysics 2023Quote: ... Mature fibers were subsequently fragmented on ice using a sonicator (Diagenode UCD-300 bath sonicator) set to high ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were lyzed and chromatin was sonicated using a Biorupto device (Diagenode) to obtain fragments with an average size ranging between 100–600 bp ...
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bioRxiv - Developmental Biology 2023Quote: ... Fragmented cDNA was used as input for library preparation with MicroPlex Library Preparation Kit v2 (Diagenode, cat. C05010012) and processed according to the manufacturer’s instruction ...
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bioRxiv - Genetics 2023Quote: ... coupled to 30µL beads was added to 400µL soluble chromatin (Diagenode H3K9me3 antibody was used in Figure 2E only ...
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bioRxiv - Genetics 2023Quote: ... For the H3K9me3 antibody (C15410193, Diagenode) preincubation was done with Streptavidin beads ...
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bioRxiv - Genetics 2023Quote: ... Pellets were resuspended in 1ml lysis buffer + 0.1% SDS + cOmplete protease inhibitor and chromatin was sheared by sonication using a Bioruptor (Diagenode (Seraing, Belgium), 8 cycles of 30sec ON/45sec OFF) ...
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bioRxiv - Genomics 2023Quote: ... H3K27Ac ChIP (Diagenode, C15410174) was performed as previously described (78) ...
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bioRxiv - Genomics 2023Quote: ... Samples were sonicated with a Bioruptor Pico (Diagenode) for 18 cycles of 30 sec ON and 30 sec OFF intervals ...
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bioRxiv - Genomics 2023Quote: ... 5 × 105 fixed nuclei were sonicated to a 200–500 bp length with the Bioruptor Pico sonicator (Diagenode). H3K27Ac ChIP (Diagenode ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 uL Tagmentase (Tn5 transposase - unloaded, Diagenode) was mixed with 20 uL annealed oligos and incubated on a thermomixer at 350 rpm ...
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bioRxiv - Molecular Biology 2023Quote: ... 13.75 uL N7-loaded Tn5 (Diagenode) was mixed with 550 uL TD buffer and 5 uL of this mixture was added to each well ...
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bioRxiv - Genomics 2023Quote: ... and subjected to gentle sonication using a Bioruptor (Diagenode) at 4°C to generate ChIP material ...
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bioRxiv - Genetics 2023Quote: ... third instar larval wing discs were prepared by boiling samples for 10 minutes in Laemmli SDS-PAGE loading buffer followed by sonication using the Bioruptor Pico sonication system (Diagenode) for 10 cycles (30 sec on ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using IPure kit v2 (Diagenode, Belgium) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were sonicated for 4 min (20 seconds on, 40 seconds off) using a Bioruptor Pico (Diagenode). After sonication samples were cleared with 1% triton X-100 and pelleted (16000 x g for 5 min at 4 °C) ...
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bioRxiv - Genomics 2023Quote: ... 12 μg of DNA was prepared for sequencing using Megaruptor 3 shearing (Diagenode, settings 19/31) and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio) ...
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bioRxiv - Molecular Biology 2023Quote: ... and chromatin was sheared by sonication using a Bioruptor (Diagenode, 9 cycles of 30 s on/off). Extracts were incubated for 2 h at room temperature with anti-V5 agarose beads (Sigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... a rabbit anti-H3K27me3 antibody (1:100; Diagenode, C15410195) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.05% Digitonin) and tagmentation was conducted by adding Tn5 transposase (Diagenode, C01070012) at 1:20 and incubated at 37C for 30min ...
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bioRxiv - Cell Biology 2023Quote: ... The tissue was homogenized with an electric grinder and sonicated using a Bioruptor® (Diagenode). The released DNA fragments were diluted in IP dilution buffer (20 mM Tris-Cl ...
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bioRxiv - Genetics 2023Quote: ... H3K27ac antibody (Diagenode) and isotype control IgG (provided in the kit ...
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bioRxiv - Genetics 2023Quote: ... the cross-linked chromatin was immunoprecipitated with ∼1μg of CTCF antibody (Diagenode), H3K4me1 antibody (Diagenode) ...
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bioRxiv - Genetics 2023Quote: ... Chromatin was sonicated using a Bioruptor Pico (Diagenode) to an average size of 200-700 bp ...
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bioRxiv - Molecular Biology 2023Quote: ... and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode). The RPFs were diluted in a final volume of 8 µl before the addition of 2 µl of Dephoshorylation Buffer ...
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bioRxiv - Genomics 2023Quote: ... Sonicate tissue using Diagenode Bioruptor Pico (10 cycles; 30 seconds on/ 30 seconds off) using 1.5mL microtubes (Diagenode C30010016) with 300uL per tube (several rounds needed) ...
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bioRxiv - Immunology 2023Quote: ... 3C DNA was sonicated using the Bioruptor (Diagenode; two pulses at low intensity for 20s), and 10 μg was used for the LAM-HTGTS step ...
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bioRxiv - Genomics 2023Quote: ... Lysates were moved to 15ml polystyrene conical tubes (Falcon) and sonicated in a Biorupter UCD-200 (Diagenode) on high for 30 seconds on and 30 seconds off for 4 sets of 5 cycles ...
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bioRxiv - Genomics 2023Quote: ... Lysates were sonicated in a Biorupter UCD-200 (Diagenode) on high for 30 seconds on and 30 seconds off for 5 cycles ...
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bioRxiv - Genomics 2023Quote: ... Samples were sonicated in a cold Bioruptor sonicator bath (Diagenode #UCD-200) at a maximum output ...
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Genetics 2023Quote: ... H3K79me3 (1:1000; Diagenode, C15410068, or 1:1000; Abcam, ab2621), H3K27me3 (1:1000 ...