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Citations for Diagenode :
501 - 550 of 569 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Cell lysates were then sonicated 20 times at 4°C (Bioruptor Plus, Diagenode, 30/30 cycles) and centrifuged at 14000 rpm for 1 minute at 4°C to remove cellular debris ...
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bioRxiv - Cancer Biology 2022Quote: ... purified gDNA from M.CviPI-treated cells was fragmented by sonication with a UCD-200 Bioruptor (Diagenode) at high level for 25 sec in 100 μl molecular-grade H2O ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed and DNA fragmented (∼500 bp) by sonication in a Bioruptor® Plus (Diagenode) for 48 × 30 s and 30 s cooling ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were incubated on ice for 10 minutes and sonicated using Bioruptor Pico sonicator (Diagenode) for 23 cycles (30s ON/30s OFF) ...
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bioRxiv - Molecular Biology 2023Quote: ... ∼10-20 million cells per shearing tube were sheared by sonication using either the Bioruptor (Diagenode UCD-200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells lysates were sonicated (15 cycles: 10 sec. on/10 sec. off) using sonicator (Bioruptor, Diagenode) and centrifuged at 12,000 g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2020Quote: Nucleosome assembly in the presence of Nap1 and ACF was carried out using a Chromatin assembly kit (C01030001) (Diagenode Inc. United States), following the manufacturer’s protocol with minor modifications to accommodate tracer DNA ...
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bioRxiv - Genomics 2022Quote: ... RRBS libraries were prepared for 23 samples with 200 ng genomic DNA according to the protocol described previously (Diagenode Premium RRBS kit) and kept at -80°C until submission to the GGBC for sequencing ...
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bioRxiv - Developmental Biology 2022Quote: ... were then incubated overnight at 4°C using primary antibodies for H3K27ac and SET in iC buffer from iDeal ChIP-seq kit for Histones (Diagenode, cod. C01010051). The next day ...
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bioRxiv - Molecular Biology 2024Quote: ... ChIP (Chromatin Immunoprecipitation) was performed using the reagents and protocol provided in the universal plant ChIP-Seq kit (Cat. No. C01010152; Diagenode, Ougree, Belgium) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Cells were then sonicated for 22 minutes at 30 seconds on/off using the Bioruptor sonicator (Diagenode) and spun down ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were sonicated 18 cycles of 30 s on and 30 s off (Bioruptor Pico, Diagenode) and insoluble material was discarded by centrifugation at 13000rpm for 20 minutes at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were disrupted by sonication (two cycles à 20 s at 4 °C) in a BioRuptor (Diagenode), and extracts were cleared by centrifugation at 15,000 x g for 1 min ...
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bioRxiv - Physiology 2022Quote: ... Chromatin from approximately 10 million cells was sheared using a sonication device (Bioruptor Pico, #2013-2019, Diagenode) and optimized to produce ~400bp fragments ...
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bioRxiv - Genomics 2021Quote: ... Cells were then sonicated for 10 cycles of 30 seconds on/off on a Bioruptor Pico (Diagenode) at 4°C ...
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bioRxiv - Genomics 2019Quote: Immunoprecipitations from 105 cells were performed using IP star robot at 4°C (indirect 200µl method, Diagenode) with an anti-BrdU antibody (10μg ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin from 10 million cells was sonicated to between 200-600 bp using a Bioruptor Plus (Diagenode). 100µL of Dynabeads Protein G (Invitrogen ...
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bioRxiv - Genomics 2022Quote: ... U2OS cells treated with Nutlin-3a or DMSO solvent control were sonicated using a Bioruptor (Diagenode, Beligum) for 10 cycles (60 seconds on and 30 seconds off with high intensity ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: A total of 100 ng of total genomic liver DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A total of 100ng of total DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 200,000 cells were centrifuged at 500g before proceeding with the nuclei isolation and transposition reaction using the ATAC-seq Kit from Diagenode (Diagenode Cat #C01080002). According to the results of the nuclei isolation optimization ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
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bioRxiv - Biophysics 2020Quote: ... Cell lysis were performed using EmulsiFlex-C3 (Avestin Europe GmbH, Mannheim, Germany) and Bioruptor UCD-200 (Diagenode, Belgium). Inclusion bodies were recovered by centrifugation of cell lysate at 7,000 x g for 1 hour at 4°C and were resuspended in 20 mL denaturing binding buffer (20 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2022Quote: ... sonicated until the lysates were clear of long DNA and other visible cell debris (Diagenode Bioruptor Plus sonicator). Lysates were processed by the Multi-Enzyme Digestion Filter Aided Sample Preparation (MED FASP ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were sonicated at high setting with 30 s on/off cycles using a Bioruptor UCD-200 (Diagenode). Lysates were cleared by centrifugation at 14000×g ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Subsequently, cells were spheroplasted according to the protocol from the Rando lab (Rando, 2010) followed by sonication (Bioruptor, Diagenode: ten cycles ...
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bioRxiv - Genomics 2019Quote: ... Cell clumps were dissociated by mild sonication for 30 s using the medium intensity setting in a Bioruptor (Diagenode). Lysing enzymes from Trichoderma harzianum (Sigma ...
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bioRxiv - Genomics 2020Quote: ... For single crosslinked cells nuclei were sonicated for 4 cycles of 30s on/30s off using a Picoruptor (Diagenode). Immunoprecipitation was carried out overnight at 4°C using 2 μg of RUNX1 antibody (ab23980 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were sonicated for 30 cycles at high setting (30s on, 30s off) using a Bioruptor Pico sonicator (Diagenode) and centrifuged at 13000 rpm for 10 min ...
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bioRxiv - Cell Biology 2021Quote: ... Pelleted cells were resuspended in 1 ml 50 mM Tris pH 7.5 and sonicated in a Bioruptor Twin (Diagenode) on LOW 30 sec ON ...
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bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
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bioRxiv - Cell Biology 2019Quote: ... or Raji iBZLF1 cells induced for 6 h were lysed in RIPA buffer and sheared with the BioRuptor (Diagenode) four times (5 min ...
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bioRxiv - Immunology 2020Quote: ... The cells were cross-linked with 1% formaldehyde for 10 minutes at RT and quenched with 2.5 M glycine (Diagenode) for 5 minutes at RT ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with mixing for 30 min at 4°C before sonicating with a Bioruptor® Pico (Diagenode). Lysates were then spun for 10 min at 15,000 g ...
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bioRxiv - Genomics 2023Quote: ... 7μL Tri-Methyl-Histone H3 (Lys27) (C36B11) Rabbit mAb (Cell Signalling Technology #9733) or 1μg negative control rabbit IgG (Diagenode) per ChIP reaction ...
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bioRxiv - Genetics 2024Quote: ... Cells were lysed by sonication for 15 cycles (high, 30 seconds on, 30 seconds off) using Bioruptor Plus (Diagenode). After a 15-minute incubation on ice and centrifugation (21,000 ✕ g ...
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bioRxiv - Systems Biology 2024Quote: ... cells were incubated on ice for 30 min and sonicated for five minutes (30 sec on/off, Diagenode Bioruptor). Afterwards ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were sonicated for 10 min at the high intensity setting (30 sec on/off) in a Bioruptor (Diagenode). The whole cell extract was adjusted to 25 mM MgCl2 and 5 mM CaCl2 and 1 µL DNAse I (30 U ...
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bioRxiv - Cell Biology 2022Quote: ... The cell lysate was transferred to 15 mL Bioruptor Pico tubes along with ~200μL of the corresponding sonication beads (Diagenode C010200031) and sonicated using a Bioruptor Pico (Diagenode B01060010 ...
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Pancreatic progenitor epigenome maps prioritize type 2 diabetes risk genes with roles in developmentbioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 × 5 min (30 sec on ...
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bioRxiv - Genomics 2020Quote: ... Cells were lysed and homogenized using a Dounce homogenizer and the lysate was sonicated in a Bioruptor® Plus (Diagenode), on high for 3 x 5 min (30 sec on ...
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bioRxiv - Molecular Biology 2020Quote: ... nucleic acids gently extracted from unfixed NTERA-2 cells were sheared by sonication (12 cycles at high power, 15s ON / 90s OFF) with a Bioruptor (Diagenode). 4.4 μg of sheared nucleic acids were incubated with 10 μg of S9.6 antibody for immunoprecipitation overnight at 4°C ...