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Citations for Diagenode :
451 - 500 of 569 citations for Rat Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... library was prepared using the EXP-NBD103 and SQK-LSK108 kits according to the manufacturer’s instructions and starting with 3 μg of 20 kb sheared DNA (Megaruptor, Diagenode) as input ...
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bioRxiv - Microbiology 2022Quote: ... July 2020 release using the SMRTbell Express Template Prep Kit v2.0 with the following modifications: genomic DNA was sheared using Speed 34 on the Megaruptor 3 (Diagenode) and an additional size selection step of 6-50kb fragments on the Blue Pippin (Sage Science ...
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bioRxiv - Plant Biology 2023Quote: ... rRNA depleted RPFs were directly used as input for the D-plex small RNA-seq kit (Diagenode cat#C05030001), according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... 25 ng of RNA in 8 µL water was input to the D-Plex Small RNA kit (Diagenode, C0503000) and libraries were prepared according to the manufacturer recommendations with the exception that 9 cycles of PCR were carried out ...
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bioRxiv - Microbiology 2019Quote: ... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... the bisulfite conversion ratio was calculated using spike-in controls provided by Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode) (supplementary Table S2 ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were sent to the Genomics Unit of the Centre for Genomic Regulation (PRBB, Barcelona) where RRBS libraries were prepared using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode), and sequenced ton an Illumina HiSeq 2000 platform in 50-bp single-end mode ...
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genomics 2019Quote: ... Chromatin from hMSCs and differentiated chondrocytes were extracted using the Diagenode iDeal histone ChIP-seq kit (Diagenode SA, Ougrée, Belgium). Extracted chromatin was sonicated using a Diagenode Bioruptor Standard or Bioruptor Pico to an average size of 200-500bp ...
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bioRxiv - Cancer Biology 2022Quote: Tumour samples with optimal chromatin fragment size (200-500 bp) were immunoprecipitated using the iDeal ChIP-seq Kit for Histones (Diagenode). Magnetic immunoprecipitation of sheared DNA-chromatin complexes (500ng ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 500 ng of total RNA was used for NGS libraries preparation with CATS mRNA-seq kit (Diagenode, Liege, Belgium) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... Then DNA was recovered by AMPure XP Beads and used for methylated DNA immunoprecipitation (MeDIP) using the Magnetic Methylated DNA Immunoprecipitation Kit (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... Libraries for reduced representation bisulfite sequencing (RRBS) were prepared from 100 ng DNA per sample using the Premium RRBS Kit (Diagenode). Final libraries were purified twice using Agencourt AMPure XP beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: H3K27ac chromatin immunoprecipitation (ChIP) experiments were performed as previously described12 using the iDeal ChIP-seq kit for histones (Cat# C01010171, Diagenode) and the H3K27ac rabbit polyclonal antibody (Abcam Cat# ab4729 ...
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bioRxiv - Genetics 2020Quote: ... 500 ng of each sample was converted into a sequencing-ready library using the CATS RNA-seq kit with rRNA depletion (Diagenode). The RNA integrity ...
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bioRxiv - Molecular Biology 2019Quote: ... or 2 ng (other antibodies) of ChIP-ed DNA was used to prepare DNA libraries with MicroPlex Library Preparation Kit v2 (Diagenode).
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bioRxiv - Plant Biology 2022Quote: ... 250 pg of immunoprecipitated (IP) or genomic (INPUT) DNA were used to prepare libraries with the MicroPlex Library Preparation kit v2 (Diagenode). Quality of libraries was validated using Agilent / Advanced Analytical fragment analyzer ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was purified as for ChIP-seq samples following the Diagenode iPure protocol using reagents from the Auto iDeal ChIP-seq kit (Diagenode). DNA quantification ...
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bioRxiv - Neuroscience 2023Quote: ... and 8 μl was used for small RNA libraries construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). Indexes were attached using the D-Plex Single Indexes for Illumina - Set A (Diagenode C05030010 ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Molecular Biology 2023Quote: Libraries were then prepared from 10 ng of RPFs using the D-Plex Small RNA-Seq kit for Illumina (Diagenode) and the D-Plex Unique Dual Indexes for Illumina – set A (Diagenode) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Genomics 2019Quote: ... 10 mM EDTA (2 x 107 cell/ml) and sonicated in a Bioruptor device (Diagenode) for 25 min at 30 s intervals ...
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bioRxiv - Molecular Biology 2019Quote: ... The cells were lysed and the chromatin was sheared in a bioruptor pico sonicator (Diagenode) by sonicating the samples for 3 or 4 cycles ...
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bioRxiv - Immunology 2019Quote: ... Nuclei from fixed cells were pelleted and used for chromatin immunoprecipitation per manufacturer’s instructions (Diagenode). Each immunoprecipitation reaction included chromatin from 5 × 106 cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Fixed cells were washed and resuspended in sonication buffer supplemented with protease inhibitors (from Diagenode) and PhosStop (Roche) ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Cells were lysed and nuclei were sheared by sonication using Bioruptor® Pico (Diagenode Inc.) to obtain DNA fragments of 200 to 500 nucleotides ...
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bioRxiv - Genetics 2021Quote: ... 7.5⍰x⍰106 cells were then homogenized and sheared by sonication using a Bioruptor (Diagenode) at high setting for 15⍰min ...
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bioRxiv - Cancer Biology 2021Quote: ... Formaldehyde-fixed cells were lysed and chromatin sheared by sonication using a Bioruptor Pico (Diagenode). IP was carried out using the 3µg of polyclonal CTCF antibody (Diagenode ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were lysed in 300 μl of RIPA buffer and sonicated with Bioruptor (Diagenode; number of cycles = 15 ...
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bioRxiv - Cancer Biology 2022Quote: ... The cross-linked cells were then lysed and sonicated using Bioruptor Plus sonication device (Diagenode) to obtain ∼300 bp chromatin ...
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bioRxiv - Genomics 2023Quote: ... Sonication of the cells was performed using a Bioruptor® Pico sonication device from Diagenode. The settings used for sonication were 40 cycles of 30 sec off and 30 sec on at 8°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were sonicated in 6 x 30 second intervals using a Bioruptor 300 Machine (Diagenode), then centrifuged for 10 minutes at 10,000g at 4°C ...
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bioRxiv - Genomics 2022Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, #B01060010) for 8 minutes ...
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bioRxiv - Genetics 2024Quote: ... Cell lysates were transferred to Diagenode 15 ml sonication tubes and sonicated (Bioruptor pico, Diagenode) 30 sec ON/ 45 sec OFF at 4°C for 60 cycles (6x10) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated for 15 min at high power with the Bioruptor sonicator (Diagenode). Protein quantification was performed using the Bradford reagent (Biorad #5000001) ...
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bioRxiv - Molecular Biology 2019Quote: ... Approximately 10 ng of bisulfite-treated single-stranded DNA was directly used for NGS libraries preparation with CATS DNA-seq Kit v1.0 (Diagenode, Liege, Belgium) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Capture and Amplification by Tailing and Switching method (CATS RNA-seq Kit v2 x24, Diagenode Cat# C05010041, Leige, Belgium). 10ng RNA was used as input and the dephosphorylation step omitted to select for 3’-OH RNA species (miRNA) ...
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bioRxiv - Plant Biology 2022Quote: ... The crosslinking was performed by vacuum infiltration (~ –950 millibars) for 20 min and ChIP-qPCR were performed using Diagenode’s Universal Plant kit (Diagenode, Seraing, Belgium). The shared chromatin was immunoprecipitated using anti-GFP antibody (Abcam 290) ...
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bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Neuroscience 2023Quote: ... Libraries were constructed from 500 pg total RNA using D-Plex Small RNA-seq Kit for Illumina and Single Indexes for Illumina - Set B (Diagenode, C05030001). RIN aXer cell sorting was determined to be between 1.2 and 7.2 (Bioanalyzer 6000 ...
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Regulation of transcription patterns, poly-ADP-ribose, and RNA-DNA hybrids by the ATM protein kinasebioRxiv - Molecular Biology 2023Quote: ... 50mg patient tissue RNA was extracted in an RNase free environment using RNA Extraction kit for Bioruptor Plus (Diagenode Cat# C20000010). The additional protocol listed for DNaseI treatment was followed for 10ug of extracted RNA per sample ...
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bioRxiv - Cell Biology 2023Quote: ... a total of 10 ng of dsDNA was purified using the MicroPlex Library Preparation kit v3 (C05010001, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... shear DNA and enhance cell disruption by using a water-bath (4°C) sonicator Bioruptor (Diagenode) at High Power for 40 min (sonication cycle ...
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bioRxiv - Biochemistry 2019Quote: ... Whole-cell lysates were prepared for immunoblotting by sonicating with a Bioruptor (Diagenode, Inc., Denville, NJ) for 3 min and boiling for 15 min.
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bioRxiv - Genetics 2019Quote: ... The cells were then sonicated in buffer iS1 using the Bioruptor® Pico sonication device (Diagenode) to achieve fragment sizes distribution below 3 kb.
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bioRxiv - Cancer Biology 2020Quote: ... cells were fixed for 10 minutes at RT in 11% formaldehyde solution in fixation buffer (Diagenode), diluted 1:10 in RPMI with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Centrifuged cell lysates were resuspended in shearing buffer and sonicated using the Pico Bioruptor device (Diagenode) for 15 minutes (30s ON/30s OFF) ...
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bioRxiv - Physiology 2020Quote: ... Cells were lysed and DNA-protein complexes were sheared using the Bioruptor Pico (Diagenode, ref B01060010) for 8 minutes ...