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Citations for Diagenode :
501 - 550 of 572 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... Primary antibodies were added as per the manufacturer’s suggested concentration for CUT&Tag (H3K27me3 (Diagenode, C15410195): 1 µg in 150 µl ...
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bioRxiv - Genomics 2022Quote: ... RRBS libraries were prepared for 23 samples with 200 ng genomic DNA according to the protocol described previously (Diagenode Premium RRBS kit) and kept at -80°C until submission to the GGBC for sequencing ...
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bioRxiv - Genomics 2024Quote: ... A total of 10 µg genomic DNA was sheared to a target fragment size of 19 kb using a Megaruptor 2 device (Diagenode, Denville, USA). In order to remove small fragments (<8kb) ...
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bioRxiv - Cancer Biology 2021Quote: The HiChIP protocol was performed as previously described (Mumbach 2016) using 7.5µg antibody to H3K27ac (Diagenode, C15410196) with the following modifications ...
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bioRxiv - Developmental Biology 2021Quote: ... 3µm-sections were immunostained using an antibody anti-histone H3 containing the trimethylated lysine 27 (H3K27me3) (Diagenode). Heat induced antigen retrieval was done using CC1 basic buffer (Ventana) ...
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bioRxiv - Genetics 2020Quote: ... Immunoprecipitation was performed using 75 µg of chromatin and 5 µg of Cas9 antibody (Diagenode #C15200229-100) or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913 ...
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bioRxiv - Genetics 2020Quote: ... that had been pre-blocked with 0.5% BSA and mixed with polyclonal CTCF antibody (C15410210-50, Diagenode) or polyclonal H3K9me3 antibody (AB_2532132 ...
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bioRxiv - Genomics 2020Quote: ... The latter aliquot was processed for ChIP using 3 μg anti-H3K4me3 antibody (pAb-003-050, Diagenode) as indicated in the user manual ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003] ...
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bioRxiv - Genomics 2023Quote: ... about half of the chromatin extract was used for immunoprecipitation with 5µg of H3K27me3 antibody (C15410069 Diagenode). IP ...
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bioRxiv - Genomics 2022Quote: ... libraries were constructed with 200 ng input DNA from each sample (n = 24; Table 1) using the Premium RRBS Kit (Diagenode, Cat. No. C02030032) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: A total of 100 ng of total genomic liver DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... ChIPseq libraries were prepared from 10 ng of double-stranded purified DNA using the MicroPlex Library Preparation kit v2 (C05010014, Diagenode s.a., Seraing, Belgium), according to manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: A total of 100ng of total DNA was diluted and processed using the Premium Reduced Representation Bisulfite Sequencing (RRBS) Kit (Diagenode, Denville, NJ, USA) as per the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2023Quote: ... High molecular weight DNA was extracted using a Circulomics Nanobind Tissue Big DNA Kit (NB-900-701-001) and sized to 15-25 kb using Bioruptor (Diagenode, Denville, NJ, USA). Sequencing libraries were constructed according to the manufacturers’ protocols and each sample was sequenced on one PacBio Sequel II 8M SMRT Cell for 30 hours in circular consensus sequencing mode to generate about 20 Gb of HiFi sequence data ...
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bioRxiv - Genomics 2020Quote: ... One µg per 100,000 cells of the desired antibody (H3K4me3 C15410003, H3K27ac C15410196, H3K27me3 C15410195, all from Diagenode) is added into each well ...
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bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The sample was then incubated with antibodies (H3K27ac, Diagenode, C15410196; H3K27me3, Cell Signaling 9733S; H3K4me3, Diagenode C15410003 premium) coupled with protein A and protein G beads (Life Technologies ...
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bioRxiv - Plant Biology 2024Quote: ... slides were incubated over night at 4°C with primary-antibody (H3K9me2: Diagenode: C15200154; H3K27me1: Diagenode: C15410045-50) diluted in 1% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Microbiology 2020Quote: ... gDNA was subjected to agarose gel electrophoresis and OD ratio tests to confirm the purity and concentration of the DNA prior to Bioruptor (Diagenode, Inc., Denville, NJ USA) fragmentation ...
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bioRxiv - Cancer Biology 2024Quote: ... boiled at 95°C for 10min and then DNA was sheared by sonication (Bioruptor® Pico, Diagenode; 4 cycles of 30s ON + 90s OFF). In both cases ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 10.0 μg of high molecular weight purified genomic DNA (final volume of 120 μl) was sheared using the Diagenode Megaruptor 3 instrument (Diagenode Inc., Denville, NJ, USA) and a two-cycle shear method at speed 33 followed by speed 34 ...
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bioRxiv - Genomics 2020Quote: ... then incubated with specific antibodies (anti-H3K27me3 Cell Signaling Technology 9733, anti-H3K36me2 CST 2901, anti-H3K27ac Diagenode C15410196), 1.5 million cells of sonicated cell lysate ...
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bioRxiv - Neuroscience 2022Quote: ... Pre-cleared chromatin lysate was immunoprecipitated by a ChIP-grade rabbit polyclonal antibody against histone H3K9me3 (Cat. # C15410193, Diagenode) with end-over-end rotation (4°C ...
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bioRxiv - Cell Biology 2022Quote: ... and DNA fragments (200 - 500 bp) were immunoprecipitated with a mouse monoclonal anti-5hmC antibody (2.5 μg per immunoprecipitation) following the protocol from Diagenode. The DNA-antibody mixture was incubated with magnetic beads overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... Sonicated chromatin was diluted ten times in ChIP Dilution Buffer and immunoprecipitation was done overnight at 4°C by incubating 2.5 μl (7 μg) of anti-H3K27ac antibody (C15410196; Diagenode) precoated on 25-μl protein A-coated magnetic beads (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... Primary antibody incubation was performed overnight at 4°C with gentle shaking using anti-H3K9me2 (Diagenode Cat. No. C15410060), Anti-Histone H3 (Sigma-Aldrich Cat ...
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bioRxiv - Microbiology 2024Quote: ... Immunoprecipitation was done with 2 µg of chromatin and 4 µg of antibody against H3K9me3 (Diagenode, C15410193, lot A2217P). Sequencing libraries were prepared from 5 ng of immunoprecipitated DNA as previously described [19 ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196; H3K4me3: Diagenode 3C15410003) coupled with Dynabeads protein A and protein G beads (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and were directly subjected to immunoprecipitation with corresponding antibodies (H3K4me3: Diagenode, C15410003; H3K27me3: Diagenode, C15410195; CTCF: Millipore 07-792). Library preparation was carried out using NEBNext Ultra II DNA Library Prep kit (NEB E7645) ...
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bioRxiv - Cancer Biology 2023Quote: ... Isolated nuclei from primary fallopian tube secretory epithelial cells bound to concalavin A beads were incubated with primary antibody that detects H3K27ac (0.7μg, Diagenode, RRID:AB_2637079) at 4℃ overnight ...
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bioRxiv - Molecular Biology 2024Quote: ... native ChIP was performed using an increasing amount of H3K4me3 antibodies (for the Abcam 0, 2, 4, 6, 10μl, for the Diagenode 0 ...
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bioRxiv - Microbiology 2023Quote: ... Two µg of high molecular weight genomic DNA (final volume of 100 µL at 20 ng/µL) were sheared using the Megaruptor3 (Diagenode inc, Denville, New Jersey, USA) at speed The DNA Damage repair ...
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bioRxiv - Genomics 2020Quote: ... Purified DNA was sonicated to obtain fragments of an average size of 300-400bp using a Bioruptor Pico (Diagenode; 8 cycles; 20s on and 60s off). 3μg of sonicated DNA was used for library preparation ...
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FOXO dictate initiation of B cell development and myeloid restriction in common lymphoid progenitorsbioRxiv - Immunology 2022Quote: ChIPseq was performed as previously described (Bouderlique et al., 2019) using H3K27Ac antibodies (Diagenode Cat #C15410196, lot A1723-0041D/2) and the ThruPLEX DNA-seq (Rubicon Genomics ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was done on chromatin corresponding to the remaining 10 000 cells using antibodies against H3K4me3 (Diagenode, cat no. C15410003) – 10% of the chromatin was used for input ...
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bioRxiv - Microbiology 2023Quote: ... chromatin-antibody complexes were washed and eluted from the beads using the iDeal ChIP-seq kit for transcription factors (Diagenode) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Click or tap here to enter text.Both strained culture plates and unstrained control plates were incubated with primary antibodies to detect H3K9me3 (Diagenode, C15200146) and HP1a (Abcam ...
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bioRxiv - Genetics 2023Quote: ... We assessed two histone marks H3K4me3 and H3K27me3 and the ChIP grade antibodies against for these marks procured from Diagenode (H3K4me3 ...
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bioRxiv - Bioengineering 2023Quote: ... Membranes were blocked using 5% BSA in 1X TBST and incubated overnight with primary antibody (anti-Cas9; 1:1000 dilution, Diagenode #C15200216 ...
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bioRxiv - Cell Biology 2023Quote: ... which were incubated overnight on a rotatory wheel at 4°C with the primary antibodies H4K20me1 (Diagenode; #C15410034; 2 μg), H3K9me3 (Diagenode ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and the appropriate amount of antibody (1ug of α-H3K27ac and 0.5ug of α-H3K4me1; Diagenode #C15410196 and #C15410194 respectively). Samples were incubated rotating at 4°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... beads were incubated for 30 min at RT with 5 µg of negative control antibody (C-, IgG from healthy rabbits, Diagenode). For immunoprecipitation ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty µl was saved as input and the remaining supernatant was then incubated at 4°C overnight with specific antibodies (5 µg of H3K27me3 Ab, Diagenode C15410069 or Millipore 07-449 ...