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Citations for Diagenode :
451 - 500 of 572 citations for DNA Mismatch Repair Protein Msh3 MSH3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2020Quote: ... 50ng of immunoprecipitated DNA or input sample were used to prepare sequencing libraries using the iDeal Library Preparation kit (Diagenode), according to manufacturer instructions ...
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bioRxiv - Genomics 2021Quote: ... we obtained 5 μg of high molecular weight genomic DNA (> 50% of fragments ≥ 40 kb) and sheared fragments to ~10 kb using the Megaruptor 3 (B06010003; Diagenode), followed by DNA repair and ligation of PacBio adapters using the SMRTbell Template Prep Kit v1.0 (100-991-900) ...
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bioRxiv - Cancer Biology 2020Quote: ... 50mM Tris-Cl and protease inhibitor (Genetix)] followed by sonication for DNA fragmentation to an average fragment length of ∼500bp using Bioruptor (Diagenode). Sheared chromatin was incubated at 4°C overnight with 4μg of primary or isotype control antibodies ...
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bioRxiv - Genomics 2022Quote: ... genomic DNA was extracted and fragmented to a mean size of 250 bp by sonication using a Bioruptor (Diagenode, Belgium), followed by adding an “A” base to the 3’ terminal ends of DNA fragments and ligating methylated adaptors to both ends of DNA fragments ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells and nuclei were subsequently lysed and chromatin was sonicated to fragment DNA to about 200-500 bp on a Bioruptor Pico sonication device (Diagenode). Sonicated chromatin was pre-cleared with same-host IgG and protein G Dynabeads (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Pellets were resuspended in 1 mL of FA lysis buffer and subjected to DNA sonication for 20 cycles of 30 secs in a Bioruptor Twin (Diagenode). Following pelleting by 15 min centrifugation at 13000 rpm ...
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bioRxiv - Systems Biology 2020Quote: ... 140mM NaCl,0.1% Sodium deoxy cholate,10mM EDTA) and lysis and DNA fragmentation were carried out using a bioruptor (Diagenode, Nextgen) for 30 cycles (30 sec on and off cycles) ...
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bioRxiv - Microbiology 2020Quote: ... the whole genomic DNA was isolated and subjected to fragmentation by sonication using Diagenode Bioruptor Pico System (Diagenode Inc. USA). The fragmented DNA was run on 1% agarose gel and the 150 to 350 bp fragments were obtained and purified ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1X protease inhibitor (Genetix) followed by DNA fragmentation through sonication to an average fragment length of ∼500 bp using Bioruptor (Diagenode) and sheared chromatin was incubated at 4°C overnight with 4µg of primary β-catenin (CST ...
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bioRxiv - Genomics 2023Quote: Two micrograms of genomic DNA were sonicated for 6 cycles (6 s on, 90 s off) at 4 °C with a Bioruptor NGS (Diagenode), generating average fragments of 1 kb ...
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bioRxiv - Genomics 2023Quote: ... Additional samples HG02554 and HG02953 were prepared for sequencing in the same way but with the following modifications: isolated DNA was sheared using the Megaruptor 3 instrument (Diagenode) twice using settings 31 and 32 to achieve a peak size of ∼15-20 kbp ...
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bioRxiv - Genetics 2023Quote: ... Briefly, DNA from activated B cells (day 4 with IL-4, anti-CD40) was sonicated to ∼1000 bp (Diagenode Bioruptor) before amplification via an Sµ-specific biotinylated primer (5’/5BiosG/CAGACCTGGGAATGTATGGT-3’) ...
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bioRxiv - Immunology 2024Quote: ... Nuclear extracts were prepared 21 and chromatin DNA was sonicated using Bioruptor Pico (Diagenode, 8 cycles, 30s on/off bursts) to generate DNA fragments ∼200-700 bp ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Several libraries were constructed using the Ligation Sequencing Kit (Nanopore LSK109) using DNA sheared to different size using a megaRuptor (Diagenode) to optimise yield and contiguity ...
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bioRxiv - Cancer Biology 2023Quote: ... Cross-linked chromatin was isolated and sonicated to generate DNA fragments averaging 200-500 bp in length by Bioruptor plus sonication device (Diagenode). Chromatin fragments were immunoprecipitated with antibodies directed against ZEB1 (1 μg ...
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bioRxiv - Microbiology 2023Quote: ... and chromosomal DNA was sheared to 100-500 bp fragments using the Bioruptor Pluswater bath sonication system (Diagenode, Liège, Belgium). Lysates were incubated with 40 μL Anti-FLAG M2 affinity gel (cat A2220 ...
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bioRxiv - Genomics 2023Quote: ... The immunoprecipitated DNA and 1% of the total input were reverse-crosslinked and eluted using the iPure v2 kit (Diagenode). For library preparation ...
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bioRxiv - Microbiology 2023Quote: ... was subjected to a brief sonication on ice for shearing the genomic DNA using Diagenode Bioruptor Plus sonicator (Diagenode Inc.). The supernatant was further centrifuged at 10000 x g for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplified circular DNA was sheared with ten cycles (on/off, 30/30) using a Bioruptor Pico with a cooler (Diagenode). Sequencing libraries were prepared using a NEBNext DNA Library prep kit (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: ... 250 pg of immunoprecipitated (IP) or genomic (INPUT) DNA were used to prepare libraries with the MicroPlex Library Preparation kit v2 (Diagenode). Quality of libraries was validated using Agilent / Advanced Analytical fragment analyzer ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified using phenol/chloroform and 2 µg of purified 3C sample was sonicated using the Bioruptor Pico (Diagenode). Biotinylated DNA was captured using MyOne Streptavidin T1 magnetic beads (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... DNA was purified from the ChIP samples (and from an aliquot of sheared chromatin as “input”) using iPure beads (Diagenode) as stated in the Auto iDeal protocol.
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bioRxiv - Systems Biology 2024Quote: Approximately 30-50 ng of purified genomic DNA in 50 µl has been subject to sonication using the Bioruptor Pico (Diagenode) for 15 cycles of (30 ON ...
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bioRxiv - Genetics 2024Quote: ... We sheared the high molecular weight (HMW) DNA to achieve consistent and narrow size ranges to 20kb using the Megaruptor 3 Shearing kit (E07010003, Diagenode). Megaruptor 3 was performed using customized settings ...
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bioRxiv - Genomics 2024Quote: ... The HMW DNA was sheared to a target size of 30kb with the DNA Fluid + needles at speed 45 for two cycles on a Megaruptor3 instrument (Diagenode).
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... 30 µg of chromatin and 4 μl of mouse anti-ERalpha monoclonal antibody (C15100066, Diagenode) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... with following appropriate antibodies at 4 °C o/n a rotating wheel: H3K27me3 (Diagenode, C15410195), RING1B (Cell Signaling ...
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bioRxiv - Genomics 2023Quote: ... The sample was then incubated with antibodies (AR: Abcam ab133273, FOXA1: Abcam ab23738, H3K27ac: Diagenode 3C15410196 ...
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bioRxiv - Genetics 2022Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of human antibody (H3K4me3 [RRID:AB_2616052, Diagenode C15410003], H3K4me1 [RRID:AB_306847, Abcam ab8895], and H3K27ac [RRID:AB_2637079, Diagenode C15410196]). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Samples were then pooled sixteen by sixteen and DNA of each pool was sheared to a mean size of ca 400 bp using the Bioruptor® Pico (Diagenode) (15sec ON / 90sec OFF for 8 cycles) ...
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bioRxiv - Neuroscience 2021Quote: ... DNA was then de-crosslinked and eluted for 4 hours at 65C before being purified using IPure beads (Diagenode Cat# C03010014). The purified DNA was then subjected to sequencing library preparation or qPCR analysis ...
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bioRxiv - Neuroscience 2021Quote: ... Chromatin immunoprecipitation was then performed on the sheared DNA using the iDeal ChIP-seq kit for transcription factors (Diagenode Cat# C01010170). One percent of chromatin was kept aside to be used as an input control ...
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bioRxiv - Genomics 2022Quote: ... HMW-DNA (25 ng/μl) was separately sheared with 30 kb and 35 kb settings using a Megaruptor 2 instrument (Diagenode SA). However ...
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bioRxiv - Plant Biology 2022Quote: ... A HiFi library was then prepared according to the “Procedure & Checklist - Preparing HiFi SMRTbell® Libraries using SMRTbell Express Template Prep Kit 2.0” manual with an initial DNA fragmentation by Megaruptor 3 (Diagenode, Belgium) and final library size binning into defined fractions by SageELF (Sage Science ...
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bioRxiv - Genomics 2024Quote: DNA concentration was normalized to 50 ng/μl and sheared to 6 Kb fragments using Megaruptor® 2 (Diagenode, Seraing, Belgium). Shearing quality was estimated with TapeStation 4200 (Agilent Technologies ...
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bioRxiv - Genomics 2023Quote: ... ≥2 ug of high molecular weight genomic DNA (more than 50% of fragments ≥40 kb) was sheared to ∼20 kb using the Megaruptor (Diagenode B06010002), followed by DNA repair and ligation of ONT adapters using the ONT Ligation Sequencing Kit (SQK-LSK109) ...
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bioRxiv - Plant Biology 2022Quote: ... and then the mixture was sonicated to shear DNA into 250 to 600 bp fragments using Bioruptor Plus sonication system (Diagenode, Belgium). The lysates were centrifuged at 20,000 × g for 10 min at 4°C ...
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bioRxiv - Plant Biology 2022Quote: ... Approximately 2 ug of genomic DNA was fragmented into 200-300 bp using the Bioruptor UCD-200 sonicator (Diagenode, Denville, NJ). Libraries were constructed using the DNA library prep kit for BGI platform (BGI ...
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bioRxiv - Systems Biology 2023Quote: ... 100 mM Tris-HCl pH 8.5) and boiled for 7 min at 95 °C before high-energy sonication to shear DNA (Diagenode Bioruptor pico). Protein concentrations were determined via a BCA assay (Thermo Fisher) ...
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bioRxiv - Genomics 2024Quote: ... a total of 10μg of high molecular weight DNA was subjected to random fragment shearing using a Megaruptor (Diagenode, NJ, USA). A long-read library (insert size ...
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bioRxiv - Molecular Biology 2024Quote: ... An input of 50 ng of genomic DNA was sheared to 6 kb - 10 kb using the Megaruptor® 3 (Diagenode) or g-TUBE (Covaris) ...
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bioRxiv - Plant Biology 2024Quote: ... 65µg of genomic DNA was split into 5 aliquots and manually sheared with the Megaruptor 3 instrument (Diagenode, P/N B06010003), according to the Megaruptor 3 operations manual ...
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bioRxiv - Physiology 2021Quote: ... Immunoprecipitation was performed using 10 μg of chromatin and 2.5 μg of antibody (H3K4me3 (Diagenode C15410003), H3K4me1 (abcam ab8895 and H3K27ac (Diagenode C15410196) ...
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bioRxiv - Cancer Biology 2020Quote: ... using 1 µg antibody (anti-H3K4me1, #ab8895, Abcam; anti-H3K27ac, #ab4729, Abcam; anti-H3K4me3, #c15410003, Diagenode). 10% of the sheared chromatins were kept aside as input samples ...
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bioRxiv - Genomics 2022Quote: ... Proximity ligated chromatin pellet was sonicated and immunoprecipitated using 5 μg of H3K27ac antibodies (Diagenode, C15410196). Immunoprecipitated fragments were Biotin labeled (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... respectively) of the primary antibodies (C15410003 and C15410196 for H3K4me3 and H3K27ac, respectively; Diagenode, Denville, NJ), 0.05% (w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... 25µg of sheared chromatin was then incubated with 4µg of antibody against H3K4me3 (Diagenode, C15410003-50) H3K36me3 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Beads were blocked with 1 µL of antibody (α-H3K27ac, Diagenode, C15410196; α-H3K4me3, Diagenode, C15410003) in antibody binding buffer for 3-4h at 4°C with rotation ...