Labshake search
Citations for Stemcell Technologies :
301 - 350 of 4196 citations for Vertical Electrophoresis Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2023Quote: H9 cells were treated with 10 uM Y-27632 (Stem Cell Technologies, 72304) for at least 2 h prior to nucleofection ...
-
bioRxiv - Immunology 2023Quote: ... cells were incubated for 6h in hypoxia incubator chamber (Stem cell Technologies, #27310) at 37°C and 1% CO2.
-
bioRxiv - Developmental Biology 2023Quote: ... cells were placed in mTeSR Plus supplemented with CloneR (Stem Cell Technologies, #05888) for 36 hours and then selected in 250 ng/ml puromycin for 36 hours ...
-
bioRxiv - Immunology 2023Quote: ... Isolated T cells were cryopreserved in CryoStor CS10 cryopreservation medium (Stem Cell Technologies).
-
bioRxiv - Microbiology 2023Quote: ... Cells were detached from a T75 using Gentle Cell Dissociation Reagent (Stemcell Technologies) and recovered in DMEM prior to staining ...
-
bioRxiv - Immunology 2023Quote: ... B cells were enriched (Human Pan-B cell Enrichment Kit [19554, StemCell Technologies]) and then stained with viability dye FVS780 (565388 ...
-
bioRxiv - Neuroscience 2023Quote: ... Stem cells were passaged as needed using ReLeSR medium (Stem Cell Technologies, 05872). Two control fibroblast lines GM03651 and GM03652 were purchased from Coriell and reprogrammed to pluripotency using Sendai viral vectors containing OCT4 ...
-
bioRxiv - Neuroscience 2023Quote: ... the cells were dissociated into a single-cell solution with Accutase (STEMCELL Technologies), and 9,000 live cells were seeded in each well of U-bottom Ultralow attachment 96-well plates (Corning ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells was spun down and resuspended in 10U Dispase (Stem Cell Technologies, #07923) and 1000U DNase (Worthington ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were further cultured with PneumaCult™-Ex Plus Medium (Stem Cell Technologies) on the upper and lower chamber 48 hours ...
-
bioRxiv - Biophysics 2023Quote: ... cells were treated at room temperature with ReLeSR (Stem Cell Technologies, Cat#: 05872) for 1 minute followed by 5 minutes at 37°C ...
-
bioRxiv - Neuroscience 2023Quote: Induced pluripotent stem cells were maintained in mTeSR+ medium (Stem Cell Technologies, 05825) on plates pre-coated with Matrigel (Corning ...
-
bioRxiv - Genetics 2023Quote: Transfected cells were dissociated into single cells with Accutase (STEMCELL Technologies, Cat#07920) and centrifuged for 4 minutes at 200 x g ...
-
bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
-
bioRxiv - Bioengineering 2023Quote: ... were cultured in ImmunoCult-XF T Cell Expansion media (Stem Cell Technologies, 10981) at 37 °C with 5% CO2 ...
-
bioRxiv - Developmental Biology 2024Quote: ... cells were split every 5-7 days with accutase (Stem Cell Technologies, #07920) (Bajpai et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... IPSC cells were dissociated as single cells with Accutase (Stemcell technologies, Cambridge, MA) and were transfected with plasmids pTALdNC-AAVS1_T2 (Addgene # 80496) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were cultured and maintained in mTeSR Plus medium (Stem Cell Technologies, Canada) on Matrigel-coated dishes (Corning ...
-
bioRxiv - Physiology 2024Quote: ... Cells where there treated with Gentle cell dissociation reagent (Stemcell Technologies, #100-0485) +1 μM Y-27632 for 10 minutes at RT ...
-
bioRxiv - Developmental Biology 2024Quote: ... hiPSC lines were dissociated into single cells using Accutase (Stem Cell Technologies #07920) and resuspended in differentiation medium N2B27 [vol:vol ...
-
bioRxiv - Developmental Biology 2024Quote: ... The transfected cells were cultured in mTeSR+ medium (Stem Cell Technologies #100-0276) containing Rock-inhibitor Y-27632 (10 µM ...
-
bioRxiv - Molecular Biology 2022Quote: ... The medium was changed daily and the cells were passaged every 4–8 days depending on confluency using Gentle Cell Dissociation Reagent (Stem Cell Technologies). 10 μM of ROCKi (Stem Cell Technologies ...
-
bioRxiv - Immunology 2019Quote: CD4+ T-cells were isolated from splenocytes and lymph node cells of wild type C57Bl/6 mice (6-8weeks old) using a T-cell negative selection kit (Easy Sep CD4+ T-cell enrichment kit, Stem Cell Technologies) to > 90% purity according to the manufactureŕs instructions ...
-
bioRxiv - Bioengineering 2020Quote: ... and iEndo-mKate2 PGP1 cells were dissociated from 70%-80% confluency by incubating cells in Gentle Cell Dissociation Reagent (STEMCELL Technologies, #07174) for 12 min at 37°C ...
-
bioRxiv - Immunology 2021Quote: NK cells were purified from uninfected spleens of WT and CypD-/- mice using the EasySep Mouse NK Cell Isolation Kit (Stem Cell Technologies) according to the supplier’s recommendations ...
-
bioRxiv - Immunology 2021Quote: ... C57BL/6.OT-I.Ubiquitin-GFP CD8+ T cells were enriched from single-cell suspensions of lymph nodes and spleens using mouse CD8-negative selection kits (Stem Cell Technologies). Enriched CD8 T cells (5×106 ...
-
bioRxiv - Cancer Biology 2020Quote: ... Splenocytes were fused with SP2/0 Ag14 myeloma cells (Kohler and Milstein, 1975) and hybrid cells were selected by growth in methylcellulose-containing HAT medium (Stem Cell Technologies). Approximately 600 clones were isolated and transferred to liquid HT medium in 96-well plates ...
-
bioRxiv - Cell Biology 2021Quote: ... DKC1 A353V (AAVS1-EGFP) iPS cells and DKC1 A353V mutation corrected (AAVS1-EGFP) iPS cells) were maintained on vitronectin (Stem Cell Tech.) coated plates using StemMACS™ iPS-Brew XF medium (Miltenyi Biotec ...
-
bioRxiv - Cell Biology 2022Quote: ... Passage one cells were seeded into T75 cell culture flasks (250,000 cells/flask) and expanded in PneumaCult ExPlus medium (StemCell Technologies, Vancouver, Canada) at 37°C ...
-
bioRxiv - Immunology 2020Quote: ... Naïve B cells were purified from the splenocyte suspension by negative isolation using an EasySep Mouse B Cell Isolation Kit from Stem Cell Technologies, according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... cells were sorted for single cells for transfected (GFP+) cells and plated into prewarmed (37C) StemFlex media supplemented with 1X CloneR (Stem Cell Technologies) onto Vitronectin XF (Stem Cell Technologies ...
-
bioRxiv - Immunology 2022Quote: ... Naïve human CD4+ T cells were isolated using the EasySep™ Human Naïve CD4+ T Cell Isolation Kit II (Stem Cell Technologies) according to the manufacturers’ instructions ...
-
bioRxiv - Immunology 2022Quote: ... after blood samples were diluted 1:1 with PBS during B cell isolation using RosetteSep Human B-Cell Enrichment Cocktail (Stem Cell Technologies). SARS-CoV-2 RBD-specific IgG ELISA tests with the plasma were performed in the lab of Dr ...
-
bioRxiv - Immunology 2020Quote: ... and CD8+ T cells were negatively isolated using the EasySep™ Mouse CD8+ T cell Isolation Kit (Stem Cell Technologies, Grenoble, France) or MojoSort™ Isolation Kits (BioLegend ...
-
bioRxiv - Immunology 2020Quote: ... Purity and viability of isolated T cells was assessed using flow cytometry prior to cryopreservation of isolated T cells in CryoStore CS10 cryopreservation media (Stem Cell Technologies) at a density of 1-1.5×107 cells per ml.
-
bioRxiv - Cancer Biology 2022Quote: ... pH 7.3] to remove red blood cells and CD8+ T cells were isolated via EasySep Mouse CD8+ T Cell Isolation Kit (Stemcell Technologies,19853) per manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... via Ficoll-Paque (Cytavia) gradient selection for peripheral blood mono-nuclear cells followed by human CD8+ T cell negative magnetic selection (Stem Cell Technologies). Primary CD8+ T cells were thawed in supplemented RPMI 1640 2-12 hours prior to the start of experiments.
-
bioRxiv - Bioengineering 2022Quote: We isolated B cells from healthy donors’ peripheral blood mononuclear cells (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell isolation kit (Stem Cell Technologies). Isolated B cells were cultured in Iscove’s modified Dulbecco’s medium (Gibco ...
-
bioRxiv - Neuroscience 2023Quote: Human iPSC-derived DA neurons were plated on a 48-well Classic MEA plate (Axion BioSystems) at a cell density of 1.2×105 cells per well and maintained in culture using Complete BrainPhys™ (Stem Cell Technologies). Culture Medium prepared as directed in the iCell DopaNeurons MEA Application Protocol (https://www.fujifilmcdi.com/) ...
-
bioRxiv - Immunology 2023Quote: ... filtered through 40 µm cell strainers and T cells enriched by negative selection using an EasySep™ pan-T cell enrichment kit (Stemcell Technologies) as per manufacturer’s guidelines ...
-
bioRxiv - Cancer Biology 2023Quote: ... After an additional 48 hours non-adhered cells were discarded and adhered T-ALL cells were separated from glioblastoma cells using EasySep Human CD3 positive Selection kit II (Stem Cell Technologies) and viability was determined by trypan blue staining (Bio-Rad) ...
-
bioRxiv - Cancer Biology 2023Quote: ... the medium containing hematopoietic cells and trypsinized cells were collected for FACS analysis and CFU-C assay in Methylcellulose M3434 (Stem Cell Technologies). After 10 days of culture at 37°C in 5% CO2 ...
-
Human CCR6+ Th cells show both an extended stable gradient of Th17 activity and imprinted plasticitybioRxiv - Immunology 2023Quote: ... CCR6low and CCR6high memory subgroups and cultured at 1 × 106 cells/ml in 24 well plates in ImmunoCult XF T cell expansion medium (Stem Cell Technologies) supplemented with 10% FBS and IL-2 (200 units/ml) ...
-
bioRxiv - Bioengineering 2023Quote: We isolated B cells from healthy human donors’ PBMCs (Fred Hutchinson Cancer Research Center) using the EasySep Human B cell enrichment kit (Stem Cell Technologies). We obtained >95% purity for B cells defined by CD3 negativity and CD19 positivity ...
-
bioRxiv - Cell Biology 2023Quote: ... Accutase was quenched and single cells were seeded for differentiation in suspension at a density of 1×106 cells/mL in complete mTeSR media (Stem Cell Technologies) supplemented with 10ng/mL FGF2 (Peprotech ...
-
bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells and isolated CD4+ T cells were activated using Immunocult Human CD3/CD28/CD2 T cell activator (StemCell Technologies, 10970) per manufacturer instructions ...
-
bioRxiv - Cancer Biology 2023Quote: ... apoptotic and dead cells were removed by immunomagnetic cell separation using the Annexin Dead Cell Removal Kit (StemCell Technologies, cat. no. 17899) and EasySep Magnet (StemCell Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... the cells that passed through 70-uM cell strainer were purified with EasySepTM Mouse CD8+ T Cell Isolation Kit (STEMCELL Technologies Inc). For T-cell proliferation assays ...
-
bioRxiv - Synthetic Biology 2023Quote: ... T cells were then resuspended at 1×106 cells/mL in ImmunoCult™-XF T Cell Expansion Medium (Stemcell Technologies, Cat.#10981) supplemented with Human Recombinant IL-2 (12.5 ng/mL ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cells were plated for single cell clones by plating 1000 cells per 10cm2 dish on Synthemax with 1x CloneR (Stemcell technologies #05888), and single cell clones were genotyped by PCR for correct integration before sequencing both alleles from positive clones.